Interferon-γ Release Assays for the Evaluation of Tuberculosis Infection

Blumberg, Henry M.; Kempker, Russell R.

doi:10.1001/jama.2014.4928

Cited by 12 publications

(13 citation statements)

References 8 publications

(12 reference statements)

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“…The low specificity of TST is related to the purified protein derivative (PPD) used for TST that encompasses abundant antigens that are nearly the same as in BCG or nontuberculous mycobacterium antigens, therefore more accurate tests, for detecting active tuberculosis is urgently needed (29). The current antigen is introduced for IGRAs to detect LTBI.…”

Section: Discussionmentioning

confidence: 99%

“…Tuberculosis and quantity FERON-tuberculosis are 2 commercially gold standard diagnostic tests for diagnosis of tuberculosis infection (29)(30)(31). Using IGRA tests, people, who were infected with tuberculosis could be distinguished from vaccinated or individuals infected with nontuberculosis mycobacteriu M. tuberculosis tests are more specific than TST because they are able to detect tuberculosis infection from BCG vaccinated individuals yet they cannot discriminate old infections from recent infections.…”

Section: Discussionmentioning

confidence: 99%

“…More recently, several studies reported the use of HBHA protein as HBHA-IGRA test for detection of latent tuberculosis disease. Heparin-binding hemagglutinin protein participates in extra pulmonary tuberculosis that may involve other organ of the body (29,32).…”

Section: Discussionmentioning

confidence: 99%

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Co-Expression of hbha and mtb32C Genes from Mycobacterium tuberculosis H37Rv in a Prokaryotic System

Teimourpour

Peeridogaheh

Arzanlou

et al. 2018

Jundishapur J Microbiol

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Background: Heparin-Binding Hemagglutinin (HBHA) protein is a surface adhesin that mediates the attachment of Mycobacterium tuberculosis to host cells by its own unique, carboxyl-terminal region. The methylated HBHA has a specific motif with a lysine-, alanine-, and proline-rich domain. More recently, it has been shown that HBHA protein has potential activity in stimulating immune responses, and is a promising new candidate for diagnostic applications besides a protective antigen against tuberculosis. Objectives: The aim of this study was to isolate a mycobacterial latency gene (hbha), and subsequently produce its protein as a new antigen for the Interferon-Gamma Release Assay test (IGRAs). Methods: In the present work, hbha and mtb32C genes were isolated from the M. tuberculosis H37Rv genome using the polymerase chain reaction (PCR) method. The PCR products and pet21+ vector were digested with specific restriction enzymes and then submitted to the ligation procedure. Escherichia coli BL21-CodonPlus (DE3) competent cells were transformed with the recombinant mtb32C-hbha -pet21+ vector. Expression of recombinant protein (Mtb32C-HBHA) was confirmed with Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and western blot methods. Results: Detection of a 500-bp gene and sequencing of recombinant pet-mtb32C-hbha vector, all confirmed the accuracy of the cloning procedure. A 36-KDa band of Mtb32C-HBHA protein was also detected by western blotting. Conclusions: In this study, expression of Mtb32C-HBHA protein was successfully done, in the prokaryotic system. Further studies are needed to evaluate the efficacy of recombinant Mtb32C-HBHA protein in diagnosis of latent tuberculosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Co-Expression of hbha and mtb32C Genes from Mycobacterium tuberculosis H37Rv in a Prokaryotic System

Teimourpour

Peeridogaheh

Arzanlou

et al. 2018

Jundishapur J Microbiol

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“…Active TB should be ruled out in patients with a positive diagnostic test for LTBI before the initiation of therapy for the treatment of LTBI [36].…”

Section: Latent Tuberculosismentioning

confidence: 99%

“…An IGRA is preferred over the TST when testing people who are BCG vaccinated or are unlikely to return for TST reading. The TST is preferred for serial testing programs, such as those involving health care workers, because the IGRA has a high false positive rate in this setting in the United States [36]. The TST is less expensive and is therefore preferred in low-income regions [33].…”

Section: Latent Tuberculosismentioning

confidence: 99%