Interferon‐α Production and Tissue Localization of Interferon‐α/β Producing Cells after Intradermal Administration of Aujeszky's Disease Virus–Infected Cells in Pigs

Abstract

Intradermal administration of glutaraldehyde-fixed Aujeszky's disease virus (ADV) infected autologous or allogeneic cells resulted in the induction of an interferon (IFN)-alpha response in pigs. Using a sensitive dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA), IFN-alpha was detected in blood at 8 and 24 h after injection of ADV-infected cells. In parallel, by means of in situ hybridization, IFN-alpha/beta mRNA containing cells were demonstrated in regional lymph nodes. Occasional IFN-alpha/beta mRNA positive cells were also seen in injected dermal areas, but not in contralateral lymph nodes, spleen, bone marrow, blood or liver. The ability of leucocytes in whole blood cultures to produce IFN-alpha upon stimulation by ADV was markedly diminished 3-7 days after intradermal injection of ADV-infected cells. In contrast, cultures of purified peripheral blood mononuclear cells (PBMC) had intact IFN-alpha responses. Further, serum from ADV-injected pigs inhibited the in vitro ADV-induced IFN-alpha responses in PBMC from control pigs, most likely due to the demonstrated presence of anti-ADV antibodies. We suggest that the IFN-alpha/beta producing cells in lymph nodes may participate in the development of antiviral immunity and could be equivalent to Natural IFN-alpha/beta producing (NIP) cells.

In vivo induction of interferon-alpha in pig by non-infectious coronavirus: tissue localization and in situ phenotypic characterization of interferon-alpha-producing cells.

“…Previous studies have shown that IFN-α SC are mainly detected in the lymphoid tissues such as the spleen or regional lymph nodes following non-infectious virus injection (Artursson et al, 1995 ;Splichal et al, 1995 ;Eloranta et al, 1996 ;Riffault et al, 1996). However, this is the first demonstration that circulating PBMC did not produce a detectable amount of IFN-α in vivo.…”

“…IFN-α SC were accordingly detected in the spleen or the draining lymph node following intravenous (i.v.) or subcutaneous injection of UV-inactivated virus, respectively (Artursson et al, 1995 ;Eloranta et al, 1996 ;Riffault et al, 1996).…”

Human recombinant interferon‐β influences T helper subset differentiation by regulating cytokine secretion pattern and expression of homing receptors

“…[24,45]. IFN-P is produced in large amounts by keratinocytes in human skin, and IFN-P mRNA can be detected in skin-draining LN after intradermal injection of virus-infected cells [46]. IFN-alp produced in the draining LN or transported to LN via afferent lymph may promote L-selectin expression which is otherwise lost during primary Tcell activation.…”

Identification of a sequence from the genome of porcine circovirus type 2 with an inhibitory effect on IFN-α production by porcine PBMCs

“…IFN-a in cell culture supernatants was quantified by a dissociation-enhanced lanthanide fluoro-immunoasssay (DELFIA), as described previously (Artursson et al, 1995). DELFIA, which is based on two mAbs directed to porcine IFN-a, had a detection limit of 0?3 U IFN-a ml…”