Interferon (type-? and ?) production by fresh and cryopreserved human mononuclear cells

The interferon (IFN) assay of the sera from the 40 patients with head and neck cancer was performed by the plaque-reduction assay with vesicular stomatitis virus in FL cells derived from human amniotic membrane. The patients mainly had Stage I11 or IV lesion without distant metastasis, and previously had not received any cancer therapy. All of the patients were histologically diagnosed as squamous cell carcinoma. When the serum IFN activity was characterized by acid treatment, significant increases of IFN-a/@/y (n = 24, P < 0.05) and acid-labile IFN (n = 24, P < 0.001), and significant decrease of acid-stable IFN (n = 24, P < 0.001) in the cancer patients of SO-to-79-year age group were found, as compared with those in the normal controls of the same age group (n = 20). When IFN titers including various immunologic parameters of the patients and normal controls were simultaneously assayed prior to the beginning of the cancer therapy, the titers of IFN-a/@/y, acid-stable IFN, and acid-labile IFN were significantly correlated with some immunologic parameters such as natural killer (NK) activity, the absolute number of Ty lymphocytes, the percentages of 8and y-globulin, and the amounts of IgA, IgG, IgM, and 6 2 microglobulin. To define further the nature of this IFN, both sera of the patients and normal donors of SO-to-79-year age group were characterized by a neutralization assay with an antiserum to HuIFN-a and HUIFN-@. The IFN activity left when the testing sera were neutralized with these antisera was expressed as y-like IFN. The titers of 7-like IFN in the sera of patients (n = 24, P < 0.0001) showed a highly significant increase as compared with the normal controls (n = 20). When the correlation between prognosis of the disease and titers of serum IFN were investigated by measuring 7-like IFN and acid-stable IFN in the sera of patients, all of nine patients with good prognosis after the cancer treatment showed significant decreased levels of y-like IFN (P < 0.01) and acid-stable IFN (P < 0.05) as compared with those on the time before cancer therapy. On the other hand, titers of ylike IFN in the sera of six patients with recurrent disease showed a significant increase as compared with those on the IFN measurement before cancer therapy (P < 0.05). These findings led us to suggest that the serum IFN levels, especially y-like IFN activity, may be associated with a function of some loops of the immune system in the patients with head and neck cancer.

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Interferon activity and its characterization in the sera of patients with head and neck cancer

Sato

Yoshida

Yanagawa

et al. 1984

Cancer

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Herpes simplex virus-induced immunespecific interferon production by cryopreserved human peripheral blood mononuclear leukocytes

Reichman

Strussenberg

Green

1986

Journal of Immunological Methods

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Potent inhibition of HIV-1 gene expression and TAT-mediated apoptosis in human T cells by novel mono- and multitarget anti-TAT/Rev/Env ribozymes and a general purpose RNA-cleaving DNA-enzyme

Unwalla¹,

Chakraborti²,

Sood³

et al. 2006

Antiviral Research

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Interferon (type-? and ?) production by fresh and cryopreserved human mononuclear cells

Cited by 6 publications

References 8 publications

Interferon activity and its characterization in the sera of patients with head and neck cancer

Interferon activity and its characterization in the sera of patients with head and neck cancer

Herpes simplex virus-induced immunespecific interferon production by cryopreserved human peripheral blood mononuclear leukocytes

Potent inhibition of HIV-1 gene expression and TAT-mediated apoptosis in human T cells by novel mono- and multitarget anti-TAT/Rev/Env ribozymes and a general purpose RNA-cleaving DNA-enzyme

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