Interferon Production by the Preimplantation Sheep Embryo

Roberts, R. Michael; Imakawa, Kazuhiko; Niwano, Yoshimi; Kazemi, Mohammad; Malathy, P.; Hansen, Thomas; Glass, Arielle; Kronenberg, Lee H.

doi:10.1089/jir.1989.9.175

Cited by 113 publications

(54 citation statements)

References 32 publications

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“…The quantity of biologically active IFNT in conditioned medium was determined using a cytopathic antiviral assay (Michael et al 2006;Roberts et al 1989). Samples were examined in duplicate by titrating in a 96-well plate (1 : 3 serial dilution).…”

Section: Analysis Of Ifntmentioning

confidence: 99%

Paradoxical effect of supplementary progesterone between Day 3 and Day 7 on corpus luteum function and conceptus development in cattle

O'Hara¹,

Forde²,

Carter³

et al. 2014

Reprod. Fertil. Dev.

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Abstract. The aim of the present study was to investigate the effect of short-term progesterone (P4) supplementation during the early metoestrous period on circulating P4 concentrations and conceptus development in cattle. The oestrous cycles of cross-bred beef heifers were synchronised using a 7-day P4-releasing intravaginal device (PRIDÒ Delta; 1.55 g P4) treatment with administration of a prostaglandin F 2a analogue (Enzaprost; CEVA Sante Animale) the day before PRIDÒ Delta removal. Only those heifers recorded in standing oestrus (Day 0) were used. In Experiment 1, heifers were randomly assigned to one of five groups: (1) control: no treatment; (2) placebo: insertion of a blank device (no P4) from Day 3 to Day 7; (3) insertion of a PRIDÒ Delta from Day 3 to Day 7; (4) insertion of a PRIDÒ Delta from Day 3 to Day 5; or (5) insertion of a PRIDÒ Delta from Day 5 to Day 7. In vitro-produced blastocysts were transferred to each heifer in Groups 2-5 on Day 7 (n ¼ 10 blastocysts per heifer) and conceptuses were recovered when heifers were killed on Day 14. Based on the outcome of Experiment 1, in Experiment 2 heifers were artificially inseminated at oestrus and randomly assigned to one of three treatment groups: (1) placebo; (2) PRID from Day 3 to Day 5; or (3) PRID from Day 3 to Day 7. All heifers were killed on Day 16 and recovered conceptuses were incubated in synthetic oviducal fluid medium for 24 h; spent media and uterine flushes were analysed for interferon-t (IFNT). In both experiments, daily blood samples were taken to determined serum P4 concentrations. Data were analysed using the PROC MIXED procedure of SAS (SAS Institute, Cary, NC, USA). Insertion of a PRID resulted in an increase (P , 0.05) in serum P4 that declined following removal. In Experiment 1, P4 supplementation from Day 3 to Day 5 (17.0 AE 1.4 mm) or Day 3 to Day 7 (11.3 AE 2.3 mm) increased conceptus length compared with placebo (2.1 AE 1.8 mm). Serum P4 was significantly lower from Day 9 to Day 14 (P , 0.05) and the weight of the Day 14 corpus luteum (CL) was lower in the PRID Day 3-7 group than the placebo or control groups. In Experiment 2, supplementation from Day 3 to Day 5 (94.0 AE 18.8 mm) or Day 3 to Day 7 (143.6 AE 20.6 mm) increased conceptus length on Day 16 compared with placebo (50.3 AE 17.4 mm). Serum P4 was significantly lower in the two supplemented groups following PRID removal compared with placebo (P , 0.05) and was associated with a lower CL weight in the Day 3-7 group. Conceptus length was strongly correlated with the IFNT concentration in the uterine flush (r ¼ 0.58; P ¼ 0.011) and spent culture medium (r ¼ 0.68; P , 0.002). The findings of the present study highlight the somewhat paradoxical effects of P4 supplementation when given in the early metoestrous period in terms of its positive effect on conceptus development and its potentially negative effects on CL lifespan.

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Section: Analysis Of Ifntmentioning

confidence: 99%

Paradoxical effect of supplementary progesterone between Day 3 and Day 7 on corpus luteum function and conceptus development in cattle

O'Hara¹,

Forde²,

Carter³

et al. 2014

Reprod. Fertil. Dev.

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“…Subsequentemente, essa proteína teve sua função antiluteolítica comprovada e associada ao reconhecimento materno da gestação. Após seu sequenciamento (IMAKAWA et al, 1987), foi renomeada IFNT (ROBERTS et al, 1989;ROBERTS et al, 1992), devido à sua semelhança estrutural com os interferons tipo I. Essa associação despertou muito interesse, pois além das funções antivirais e imunomoduladoras conhecidas dos interferons, essa proteína possuía ação no contexto reprodutivo.…”

Section: Receptores De Prostaglandinasunclassified

Função do interferon-tau durante o reconhecimento materno da gestação em ruminantes

Antoniazzi¹,

Henkes

Oliveira³

et al. 2011

Cienc. Rural

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RESUMO O reconhecimento materno da gestação é o período em que o concepto sinaliza sua presença para a mãe. Em ruminantes, este período coincide com o alongamento do embrião e a máxima produção de interferon-tau (IFNT ABSTRACT Maternal recognition of pregnancy is the period when the conceptus signals its presence to the dam. In ruminants, it requires conceptus elongation, which coincides with maximum production of interferon-tau (IFNT). Conceptus IFNT acts in a paracrine manner silencing estrogen receptor alpha (ESR1) and oxytocin receptor (OXTR) in the luminal

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“…IFNτ also exhibits 1 × 10 8 units of antiviral activity/mg protein and possesses an antiproliferative effect like other IFNs [34][35][36]. In addition, IFNτ strongly inhibits incorporation of [ 3 H]-thymidine into lymphocyte DNA when lymphocytes are exposed to various mitogens or to genetically unrelated lymphocytes, suggesting that IFNτ has an immunosuppressive effect [34,37].…”

Section: Biological Properties Of Ifnτ τ τ τmentioning

confidence: 99%

Regulation of Interferon-.TAU. Gene Expression and the Maternal Recognition of Pregnancy.

Yamaguchi

Nagaoka

Matsuda

et al. 2001

Journal of Reproduction and Development

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Abstract. In order for pregnancy to be established, the maternal system must recognize the presence of a conceptus(es) within the uterus. This physiological phenomenon, which subsequently causes the extension of the corpus luteum (CL) life-span, is known as maternal recognition of pregnancy. In ruminant ungulates, interferon-tau (IFNτ), produced by the conceptus, binds to the receptor located in the maternal endometrium in a paracrine manner. Its binding then affects levels of endometrial oxytocin receptor and estrogen receptor, which reduces pulsatile secretion of a luteolysin, prostaglandin F2α, resulting in the extension of CL function. Production of IFNτ seems to parallel the degree of trophoblast elongation and as the conceptus begins its attachment to the endometrium, IFNτ levels decline rapidly. Involvement of IFNτ in the process of implantation has long been suspected, but a role for IFNτ other than CL extension or transcriptional regulation of IFNτ genes has not been elucidated. In fact, several transcription factors that regulate the expression of IFNτ have been found, but none could explain its unique regulation. Recent findings related to maternal recognition of pregnancy are presented herein and a new insight into the potential regulation of IFNτ is discussed.

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Interferon Production by the Preimplantation Sheep Embryo

Cited by 113 publications

References 32 publications

Paradoxical effect of supplementary progesterone between Day 3 and Day 7 on corpus luteum function and conceptus development in cattle

Paradoxical effect of supplementary progesterone between Day 3 and Day 7 on corpus luteum function and conceptus development in cattle

Função do interferon-tau durante o reconhecimento materno da gestação em ruminantes

Regulation of Interferon-.TAU. Gene Expression and the Maternal Recognition of Pregnancy.

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