Interferon- -loaded collagen scaffolds reduce myofibroblast numbers in rat palatal mucosa

Abstract

Wound contraction and scar formation after cleft palate repair lead to growth impairment of the maxilla and midface. Myofibroblasts play a key role in these processes. The application of an interferon-γ (IFN-γ)-loaded collagen scaffold after surgery might reduce the differentiation of myofibroblasts. In this study, the tissue response to IFN-γ-loaded collagen scaffolds was evaluated after implantation in the palate of rats. Scaffolds, with or without IFN-γ, were implanted submucoperiosteally in the palate of two groups of 25 five-week-old male Wistar rats. Groups of five rats were sacrificed at 1, 2, 4, 8, and 16 weeks post-implantation and processed for histological analyses. On haematoxylin and eosin-stained sections, the cell density and number of giant cells within the scaffolds were determined. Blood vessels, inflammatory cells, and myofibroblasts were detected by immunohistochemistry. The data for cell density, blood vessels, and giant cells were compared with a two-way analysis of variance. The scores for myofibroblasts and inflammation were compared by a rank sum test. A mild and rapidly subsiding inflammatory and foreign body response was found in both groups. Angiogenesis had already begun after 1 week, showed a peak after 4 weeks, and declined thereafter. IFN-γ induced a faster influx of host cells and a major reduction in myofibroblast numbers. The scaffolds might be suitable for future applications in oral surgery.

Reliability of overbite depth indicator (ODI) and anteroposterior dysplasia indicator (APDI) in the assessment of different vertical and sagittal dental malocclusions: a receiver operating characteristic (ROC) analysis

“…The APDI is the sum of three interplaner angles that showed the highest correspondence with Angle's classification 12 13 , 14 …”

“…, 11 , 14 However, to date, no study has been conducted in a Pakistani population. Therefore, the aim of our study was to determine and compare the mean ODI and APDI values in various overbite and Angle's classification groups, respectively.…”

Factors associated with the prevalence of anterior open bite among preschool children: A population-based study in Brazil

“…Anterior open bite (AOB) and posterior crossbite have been identified as the most common occlusal abnormalities in primary dentition. 4 , 5 AOB is characterized by lack of occlusal contact in the anterior region, while the remaining teeth are in occlusion. 6 , 7 AOB is more prevalent in primary dentition, with a prevalence between 6.2% and 50.0% worldwide, varying according to the population group studied.…”

“… 6 , 7 AOB is more prevalent in primary dentition, with a prevalence between 6.2% and 50.0% worldwide, varying according to the population group studied. 3 , 4 , 5 , 8 - 11 This is most likely to be associated with an increase in overbite during the mixed dentition period, and the self-correcting nature of the majority of cases of anterior open bite in primary dentition. 5 , 12 …”

Collagen Scaffolds in Bone Sialoprotein‐Mediated Bone Regeneration

“…For example, acellular type 1 collagen-heparin scaffolds containing fibroblast growth factor 2 (FGF2) and vascular endothelial growth factor (VEGF) can stimulate angiogenesis, neovascularization and vascular tissue regeneration in vivo [29, 30]. To reduce wound contraction and scarring following cleft palate surgery, Jansen et al developed interferon- γ -loaded collagen scaffolds that significantly diminished myofibroblast influx/differentiation following palate surgery in Wistar rats [31]. …”