Interferon gamma response to Mycobacterium avium subsp. paratuberculosis specific lipopentapeptide antigen L5P in cattle

Holbert, Sébastien; Branger, Maxime; Souriau, Armel; Lamoureux, Bérénice; Ganneau, Christelle; Richard, G.; Cochard, Thierry; Tholoniat, Christophe; Bay, Sylvie; Winter, Nathalie; Moyen, Jean Louis; Biet, Franck

doi:10.1016/j.rvsc.2015.07.017

Cited by 13 publications

(15 citation statements)

References 23 publications

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“…MLVA genotyping confirmed the identification of the species Map. The MLVA profile of the DNA was identical to that of an isolated strain from feces of a Map naturally infected cattle in the same farm (Holbert et al, 2015 ). These results not only confirm the presence of FLA harboring Map in the environment of animals on farm, but the identical genotypes recovered may also suggest a link between residence in amoeba and ability to infect cattle.…”

Section: Discussionmentioning

confidence: 74%

“…The Map strains K10, 397, and 7912 were used as positive control and M. llatzerenze as negative control for all PCR-based assays (Table 3 ). We have previously established a database containing isolated and genotyped Map strains from feces of infected cattle (Holbert et al, 2015 ). Therefore, we genotyped the Map-positive DNA from the amoeba culture, using MLVA genotyping and compare it to our database.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Environmental Mycobacterium avium subsp. paratuberculosis Hosted by Free-Living Amoebae

Samba-Louaka

Robino

Cochard

et al. 2018

Front. Cell. Infect. Microbiol.

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Mycobacterium avium subsp. paratuberculosis is responsible for paratuberculosis in animals. This disease, leading to an inflammation of the gastrointestinal tract, has a high impact on animal health and an important economic burden. The environmental life cycle of M. avium subsp. paratuberculosis is poorly understood and several studies suggest that free-living amoebae (FLA) might be a potential environmental host. FLA are protozoa found in water and soil that are described as reservoirs of pathogenic and non-pathogenic bacteria in the environment. Indeed, bacteria able to survive within these amoebae would survive phagocytosis from immune cells. In this study, we assessed the in vitro interactions between several strains of M. avium subsp. paratuberculosis and Acanthamoeba castellanii. The results indicate that the bacteria were able to grow within the amoeba and that they can survive for several days within their host. To explore the presence of M. avium subsp. paratuberculosis in environmental amoebae, we sampled water from farms positive for paratuberculosis. A M. avium subsp. paratuberculosis strain was detected within an environmental amoeba identified as related to the poorly described Rosculus genus. The bacterial strain was genotyped, showing that it was similar to previous infectious strains isolated from cattle. In conclusion, we described that various M. avium subsp. paratuberculosis strains were able to grow within amoebae and that these bacteria could be found on farm within amoebae isolated from the cattle environment. It validates that infected amoebae might be a reservoir and vector for the transmission of M. avium subsp. paratuberculosis.

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Section: Discussionmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Environmental Mycobacterium avium subsp. paratuberculosis Hosted by Free-Living Amoebae

Samba-Louaka

Robino

Cochard

et al. 2018

Front. Cell. Infect. Microbiol.

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“…avium raises difficulties for specific immunodetection with possible false-positive reactions resulting from the presence of both strains in the environment. In the last years, analyses of mycobacterial genomes permitted to indentify the production of lipopentapeptide (L5P) in MAP as a distinctive feature among subspecies [ 13 ]. Studies in MAP-infected ruminants showed a strong immunogenicity of L5P [ 14 ] and, more recently, highly specific IgG responses to L5P were verified in patients affected by Crohn’s disease [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Seroreactivity against Specific L5P Antigen from Mycobacterium avium subsp. paratuberculosis in Children at Risk for T1D

et al. 2016

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Aims/HypothesisAlthough numerous environmental agents have been investigated over the years as possible triggers of type 1 diabetes (T1D), its causes remain unclear. We have already demonstrated an increased prevalence of antibodies against peptides derived from Mycobacterium avuim subsp. paratuberculosis (MAP) homologous to human zinc transporter 8 protein (ZnT8) and proinsulin in Italian subjects at risk for or affected by T1D. In this study, we compared titers of the previously detected antibodies with seroreactivity to MAP lipopentapetide (L5P) that recently emerged as a strong immunogenic component able to specifically distinguish MAP from other mycobacteria.MethodsPlasma of 32 children and youth at risk for T1D including follow-up samples and 42 age-matched healthy controls (HC) recruited at the Tor Vergata University Hospital in Rome was analyzed by indirect ELISA for the presence of antibodies against MAP-derived epitopes MAP3865c133–141, MAP3865c125-133, MAP2404c70-85 and MAP1,4αgbp157-173 along with their ZnT8 and proinsulin homologs. The data were analyzed through two-tailed Mann-Whitney U test and relation between variables was determined by principal component analysis.ResultsResponses to L5P were not detectable in subjects whose initial seroreactivity to MAP peptides and their human homologs was lost in follow-up samples, whereas anti-L5P antibodies appeared constantly in individuals with a stable immunity against MAP antigens. The overall coincidence in positivity to L5P and the four MAP epitopes both in children at risk for T1D and HC exceeded 90%.ConclusionsMAP-derived homologs may cross-react with ZnT8 and proinsulin peptides inducing immune responses at a young age in subjects predisposed for T1D. Thus, L5P may have a diagnostic value to immediately indicate the presence of anti-MAP seroreactivity when evaluation of a more complex antibody status is not required. Almost complete coincidence in responses to both types of antigens lends support to the involvement of MAP in T1D.

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“…avium (Eckstein et al ., ). Our group has previously demonstrated that L5P is antigenic in antibody‐based tests (Biet et al ., ; Verdier et al ., ) with negligible cell‐mediated immune responses (Holbert et al ., ). In the present study, L5P preferentially resulted in the upregulation of activated B cells (CD25 + B cells), a finding that correlates with previous studies demonstrating this lipopentapeptide produces strong humoral responses in cattle and sheep (Biet et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Cell wall peptidolipids of Mycobacterium avium: from genetic prediction to exact structure of a nonribosomal peptide

Bannantine

Etienne

Laval

et al. 2017

Molecular Microbiology

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Mycobacteria have a complex cell wall structure that includes many lipids; however, even within a single subspecies of Mycobacterium avium these lipids can differ. Total lipids from an M. avium subsp. paratuberculosis (Map) ovine strain (S-type) contained no identifiable glycopeptidolipids or lipopentapeptide (L5P), yet both lipids are present in other M. avium subspecies. We determined the genetic and phenotypic basis for this difference using sequence analysis as well as biochemical and physico-chemical approaches. This strategy showed that a nonribosomal peptide synthase, encoded by mps1, contains three amino acid specifying modules in ovine strains, compared to five modules in bovine strains (C-type). Sequence analysis predicted these modules would produce the tripeptide Phe-N-Methyl-Val-Ala with a lipid moiety, termed lipotripeptide (L3P). Comprehensive physico-chemical analysis of Map S397 extracts confirmed the structural formula of the native L3P as D-Phe-N-Methyl-L-Val-L-Ala-OMe attached in N-ter to a 20-carbon fatty acid chain. These data demonstrate that S-type strains, which are more adapted in sheep, produce a unique lipid. There is a dose-dependent effect observed for L3P on upregulation of CD25+ CD8 T cells from infected cows, while L5P effects were static. In contrast, L5P demonstrated a significantly stronger induction of CD25+ B cells from infected animals compared to L3P.

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Interferon gamma response to Mycobacterium avium subsp. paratuberculosis specific lipopentapeptide antigen L5P in cattle

Cited by 13 publications

References 23 publications

Environmental Mycobacterium avium subsp. paratuberculosis Hosted by Free-Living Amoebae

Environmental Mycobacterium avium subsp. paratuberculosis Hosted by Free-Living Amoebae

Seroreactivity against Specific L5P Antigen from Mycobacterium avium subsp. paratuberculosis in Children at Risk for T1D

Cell wall peptidolipids of Mycobacterium avium: from genetic prediction to exact structure of a nonribosomal peptide

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