Interferon Gamma Response to Combinations 38 kDa/CFP‐10, 38 kDa/MPT‐64, ESAT‐6/MPT‐64 and ESAT‐6/CFP‐10, Each Related to a Single Recombinant Protein of <i>Mycobacterium tuberculosis</i> in Individuals from Tuberculosis Endemic Areas

Tavares, Ricardo Candido Oliveira; Salgado, Jorge; Moreira, Valéria Barbosa; Ferreira, María Auxiliadora Pantoja; Mello, Fernanda C. Q.; Leung, Janaína W.; Fonseca, Leila de Souza; Spallek, Ralf; Singh, Mandeep; Saad, Maria Helena Féres

doi:10.1111/j.1348-0421.2007.tb03910.x

Cited by 19 publications

(27 citation statements)

References 29 publications

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“…Nonetheless, the immune dominancy of the 38-kDa peptides in a TB infection has been reported (12,(35)(36)(37)(38)(39), and comparative analyses have demonstrated that there is a quantitative difference in the levels of expression of the 380-kDa protein in M. bovis versus M. tuberculosis. It has been found, for example, that the M. bovis soluble extract had to be at least 10 times more concentrated than the M. tuberculosis soluble extract to obtain the same level of binding via Western blotting and ELISA (36).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Gamma Interferon Immune Response Elicited by the Newly Constructed PstS-1(285-374):CFP10 Fusion Protein To Detect Mycobacterium tuberculosis Infection

Araujo

Mello

Silva

et al. 2014

Clin Vaccine Immunol

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The PstS1 antigen is highly immunogenic, principally when combined with CFP10 during both latent and active TB infection. In the present study, a selected pstS1 gene fragment was cloned, fused with CFP10, and expressed in Escherichia coli. The product M ycobacterium tuberculosis, a highly successful parasite, is the cause of tuberculosis (TB). A major health care concern, the disease results in approximately 1.4 million annual deaths (990,000 among HIV-negative individuals), infecting roughly one-third of the world's population. Among the infected, 5% to 10% will develop active disease in their lifetimes and 90% will harbor the latent form. According to a recent mathematical projection of TB eradication, the treatment of latent TB infection (LTBI) and active TB is urgently required in order to lower and ultimately prevent the further spread of the disease at its present rate (1, 2).Two principal approaches based on the adaptive immune responses elicited by M. tuberculosis infection are currently used to identify TBI (3): the in vivo tuberculin skin test (TST) and the ex vivo gamma interferon (IFN-␥) release assay (IGRA).Developed in 1908, TST became the standard means of assessing the presence of TB infection. Via TST, prior TB exposure is measured by a type 4 delayed-type hypersensitivity reaction when a purified protein derivative (PPD) of M. tuberculosis is injected intradermally. Although TST has some biological limitations such as the occurrence of anergies in one-third of active TB cases, crossreactivity with M. bovis bacillus Calmette-Guérin (BCG) and non-TB mycobacteria does not, according to some authors, result in major sensibility differences in IGRAs (4).The IGRA was recently developed using antigens that are expressed in M. tuberculosis but not in BCG or M. bovis strains as stimuli. These antigens measure the production of IFN-␥ in peripheral blood mononuclear cells (3,5). According to a recent World Health Organization (WHO) report (1), the two currently commercially available IGRAs have yet to generate sufficient data or enough high-quality evidence regarding their performance in the low-and middle-income countries that typically have a high TB and/or HIV burden and where the coverage of BCG vaccination may cause some interference (5, 6).Both IGRAs are based on Mycobacterium tuberculosis-specific antigens, namely, early secretory antigenic target 6 (ESAT-6) and culture filtrate protein 10 (CFP10) (5). These antigens must be validated in different scenarios, but other antigen combinations also need to be evaluated to improve IGRA coverage.The 38-kDa M. tuberculosis antigen, also known as phosphatespecific transporter-1 (PstS-1), coded by a pstS1 gene that composes one of the 3 putative pst operons, is a lipoprotein phosphate transport receptor on the cell surface. These operons probably constitute a subtle biochemical adaptation of M. tuberculosis, enabling it to grow and survive under different phosphate-limiting conditions during its infectious cycle. The PstS-1 protein is ac-

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Section: Discussionmentioning

confidence: 99%

“…The odds ratios (OR) and 95% confidence intervals (CI) were calculated. Since the LSA cutoff had been established in previous studies (12), a receiver operating characteristic (ROC) curve using TST (cutoff of 5 mm) as a standard was done to establish the WBA cutoff points.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Gamma Interferon Immune Response Elicited by the Newly Constructed PstS-1(285-374):CFP10 Fusion Protein To Detect Mycobacterium tuberculosis Infection

Araujo

Mello

Silva

et al. 2014

Clin Vaccine Immunol

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“…This was considered the common cut-oV for all the groups. Those values which were above this cut-oV point were considered as positive [21,22]. Overall IFN-response among various groups was calculated by subtracting the unstimulated mean values from stimulated culture mean values.…”

Section: Discussionmentioning

confidence: 99%

“…Pulmonary tuberculosis patients before treatment (n = 21) All pulmonary tuberculosis patients before treatment (PTB) were positive for sputum smears and culture. The subjects of this group were naïve for anti-tuberculous treatment.…”

Section: Tuberculosis Patients (N = 34)mentioning

confidence: 99%

Cellular immune response to Mycobacterium tuberculosis-specific antigen culture filtrate protein-10 in south India

Kumar

Sundaramurthi

Mehra

et al. 2009

Med Microbiol Immunol

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The Mycobacterium tuberculosis (M. tuberculosis)-specific culture filtrate protein-10 (CFP-10) is highly recognized by M. tuberculosis infected subjects. In the present study, the proliferative response and IFN-gamma secretion was found for C-terminal peptides of the protein (Cfp6(51-70), Cfp7(61-80), Cfp8(71-90), and Cfp9(81-100)). The alleles HLA DRB1 *04 and HLA DRB1 *10 recognized the C-terminal peptides Cfp7, Cfp8, and Cfp9 in HHC. Cfp6 was predominantly recognized by the alleles HLA DRB1 *03 and HLA DRB1 *15 by PTB. The minimal nonameric epitopes from the C-terminal region were CFP-10(56-64) and CFP-10(76-84). These two peptides deserve attention for inclusion in a vaccine against tuberculosis in this region.

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“…Blood samples were drawn, and PBMCs were prepared as described by Tavares et al (7) In-vitro stimulation of 10 6 cells/well was accomplished via incubation, for three days, with a suspension containing 10 5 bacilli/well, and, as a positive control, 5 µg/mL of PHA (Sigma, St. Louis, MO, USA). Supernatants of bacteria-free cell cultures were collected and stored immediately at −70°C for IFN-γ quantification using ELISA (R&D Systems, Minneapolis, MN, USA).…”

Section: Brief Communicationmentioning

confidence: 99%

Heterogeneidade de resposta por IFN-γ a cepas clínicas de Mycobacterium tuberculosis em humanos

et al. 2010

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Mycobacterium tuberculosis is one of the most successful human pathogens. Highly virulent strains, which are more easily transmitted than are less virulent strains, elicit variable immune responses. We evaluated the Th1 responses (IFN-γ production) in healthy volunteers after stimulation with various strains. Our results show that the individuals with negative tuberculin skin test (TST) results were not necessarily naive to all of the strains tested, whereas individuals with positive TST results did not respond to all of the strains tested. Drug-resistant strains induced a lower mean level of IFN-γ production than did drug-sensitive strains. One possible practical application of this finding would be for the prediction of responses to treatment, in which it might be advantageous to have knowledge of the estimated IFN-γ production elicited by a specific isolated strain.Keywords: Tuberculosis; Polymorphism, restriction fragment length; Mycobacterium tuberculosis; Immunity, cellular; Interferon-gamma. ResumoMycobacterium tuberculosis é um dos mais bem sucedidos patógenos do homem. As cepas virulentas são mais facilmente transmitidas, induzindo respostas imunes variáveis. Avaliamos a resposta celular tipo Th1, através da produção de IFN-γ, como resposta a cepas com padrões diversos em voluntários sadios. Nossos resultados mostraram que indivíduos com teste tuberculínico (TT) negativo já tiveram contato com algumas das cepas testadas, ao passo que indivíduos com TT positivo não responderam a todas as cepas testadas. Cepas resistentes induziram uma média menor de produção de IFN-γ que aquelas sensíveis. Uma possível aplicação prática disto seria que a produção de IFN-γ, em relação a uma cepa isolada específica, poderia auxiliar na previsão da resposta ao tratamento dos pacientes.

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Interferon Gamma Response to Combinations 38 kDa/CFP‐10, 38 kDa/MPT‐64, ESAT‐6/MPT‐64 and ESAT‐6/CFP‐10, Each Related to a Single Recombinant Protein of Mycobacterium tuberculosis in Individuals from Tuberculosis Endemic Areas

Cited by 19 publications

References 29 publications

Evaluation of Gamma Interferon Immune Response Elicited by the Newly Constructed PstS-1(285-374):CFP10 Fusion Protein To Detect Mycobacterium tuberculosis Infection

Evaluation of Gamma Interferon Immune Response Elicited by the Newly Constructed PstS-1(285-374):CFP10 Fusion Protein To Detect Mycobacterium tuberculosis Infection

Cellular immune response to Mycobacterium tuberculosis-specific antigen culture filtrate protein-10 in south India

Heterogeneidade de resposta por IFN-γ a cepas clínicas de Mycobacterium tuberculosis em humanos

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