Interferon gamma release assay for differentiating tuberculosis among pneumonia cases in acute healthcare setting

Lui, Grace; Lee, Nelson; Cheung, S.W.; Lam, James; Wong, Billy; Choi, Kin Wing; Wong, Kam-Fai; Wong, Ricky W.K.; Cockram, C. S.; Hui, David S.; Chan, Raymond K. H.

doi:10.1016/j.jinf.2011.04.011

Cited by 10 publications

(15 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar sensitivities were demonstrated in the studies of Winqvist et al (52%) Dewan et al (60%) and Lui et al (60%) [16], [17], [18]. The specificity of the interferon test (87%) was also similar or even higher than that reported in numerous publications [9], [19], [20], [21].…”

Section: Discussionsupporting

confidence: 87%

Interferon-Gamma Assay in Combination with Tuberculin Skin Test Are Insufficient for the Diagnosis of Culture-Negative Pulmonary Tuberculosis

Włodarczyk

Rudnicka

Janiszewska-Drobińska³

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

ObjectiveEarly diagnosis of infectious cases and treatment of tuberculosis (TB) are important strategies for reducing the incidence of this disease. Unfortunately, traditional TB diagnostic methods are time-consuming and often unreliable. This study compared the accuracy and reliability of the tuberculin skin test (TST) and interferon (IFN)-γ-based assay (IGRA) for the diagnosis of active pulmonary TB Polish cases that could or could not be confirmed by M. tuberculosis (M.tb) culture.MethodsIn total, 126 adult patients with clinically active TB or non-mycobacterial, community-acquired lung diseases (NMLD) hospitalised at the Regional Specialised Hospital of Tuberculosis, Lung Diseases and Rehabilitation in Tuszyn, Poland were enrolled in the present study. Sensitivity, specificity, positive predicted value (PPV), negative predicted value (NPV), and analytic accuracy (Acc) of TST and IGRA testing for the diagnosis of culture-positive and culture-negative TB patients were calculated. The quantities of IFN-γ produced in the response to M.tb specific antigens (TB Ag – Nil) in the cultures of blood from patients with active TB and NMLD patients were also analysed.ResultsThe IGRA sensitivity in culture-positive and culture-negative TB patients was similar, measuring 65.1% and 55.6%, respectively. The sensitivity of TST did not differ from the parameters designated for IGRA, measuring 55.8% in culture-positive and 64.9% in culture-negative TB. The sensitivity of TST and IGRA was age-dependent and decreased significantly with the age of the patients. No differences in the frequency or intensity of M.tb-stimulated IFN-γ production, as assessed by IGRA testing between culture-positive and culture-negative TB were noticed. Significantly lower concentrations of IFN-γ were observed in patients with advanced TB forms compared with those with mild or moderate TB pathologies.ConclusionsOur results do not show that a combination of IGRA and TST might be a step forward in the diagnosis of culture-negative TB cases. However, M. tuberculosis-stimulated IFN-γ levels might help to assess the extent of pulmonary TB lesions.

show abstract

Section: Discussionsupporting

confidence: 87%

Interferon-Gamma Assay in Combination with Tuberculin Skin Test Are Insufficient for the Diagnosis of Culture-Negative Pulmonary Tuberculosis

Włodarczyk

Rudnicka

Janiszewska-Drobińska³

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…In the present study, which was conducted in a TB-endemic area, QFT-GIT had a moderate to high sensitivity (90.5%) and poor specificity (64.7%) in diagnosing active PTB. In line with previous reports, although the NPV was high (93.5%) in our study, the PPV was only 54.8% (Diel et al, 2012;Lui et al, 2011). It is plausible that the value of IGRA in diagnosing active TB is limited in TB suspects because the prevalence of LTBI could be pretty high in TB-endemic areas, and both active PTB and LTBI could lead to positive IGRA results.…”

Section: Discussionsupporting

confidence: 88%

“…It is plausible that the value of IGRA in diagnosing active TB is limited in TB suspects because the prevalence of LTBI could be pretty high in TB-endemic areas, and both active PTB and LTBI could lead to positive IGRA results. However, the ability of IGRA to exclude active TB based on its high NPV remains an issue of controversy and deserves further evaluation (Lui et al, 2011). According to 1 recent report, aging, emaciation, human immunodeficiency virus (HIV) co-infection, and HLA genotype were clinical factors associated with IGRA negativity in active TB cases (Hang et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Characteristics of IFN-γ responses in IGRA among pulmonary TB suspects in a TB-endemic area

Feng

Huang

Lee

et al. 2013

Diagnostic Microbiology and Infectious Disease

View full text Add to dashboard Cite

“…6 A global epidemiology study on anti-tuberculosis drug resistance surveillance in 1999e2002 indicated that the prevalence of MDR-TB among TB cases in Hong Kong was 0.8%. 7 The sensitivity and specificity of microscopic examination of sputum for AFB, clinical diagnosis and Xpert were retrieved from systematic review, 8 clinical diagnostic guideline validation study 9 and prospective study, 5 respectively.…”

Section: Clinical Inputsmentioning

confidence: 99%

Cost-effectiveness analysis of the Xpert MTB/RIF assay for rapid diagnosis of suspected tuberculosis in an intermediate burden area

You

Lui

Kam

et al. 2015

Journal of Infection

View full text Add to dashboard Cite

Interferon gamma release assay for differentiating tuberculosis among pneumonia cases in acute healthcare setting

Cited by 10 publications

References 37 publications

Interferon-Gamma Assay in Combination with Tuberculin Skin Test Are Insufficient for the Diagnosis of Culture-Negative Pulmonary Tuberculosis

Interferon-Gamma Assay in Combination with Tuberculin Skin Test Are Insufficient for the Diagnosis of Culture-Negative Pulmonary Tuberculosis

Characteristics of IFN-γ responses in IGRA among pulmonary TB suspects in a TB-endemic area

Cost-effectiveness analysis of the Xpert MTB/RIF assay for rapid diagnosis of suspected tuberculosis in an intermediate burden area

Contact Info

Product

Resources

About