Interferon and c- ets -1 Genes in the Translocation (9;11)(p22;q23) in Human Acute Monocytic Leukemia

208

101

Translocations involving chromosome 11, band q23, are frequent recurring abnormalities in human acute lymphoblastic and acute myeloid leukemia. We used 19 biotinlabeled probes derived from genes and anonymous cosmids for hybridization to metaphase chromosomes from leukemia cells that contained four translocations involving band 1 Iq23: t(4;11)(q21;q23), t(6;11)(q27;q23), t(9;11)(p22;q23), and t(11;19)(q23;pl3). The location of the cosmid probes relative to the breakpoint in 11q23 was the same in all translocations. Of the cosmid clones containing known genes, CD3D was proximal and PBGD, THYI, SRPR, and ETSI were distal to the breakpoint on 11q23. Hybridization of genomic DNA from a yeast clone containing yeast artificial chromosomes (YACs), that carry 320 kilobases (kb) of human DNA including CD3D and CD3G genes, showed that the YACs were split in all four translocations. These results indicate that the breakpoint at 11q23 in each of these translocations occurs within the 320 kb encompassed by these YACs; whether the breakpoint within the YACs is precisely the same in the different translocations is presently unknown)

Section: -_ Abstractsupporting

confidence: 72%

“…We have previously studied the 9;11 translocation by using probes for the interferon gene cluster on 9p and for the ETSJ gene (20). Our data appeared to show that the a-interferon (IFNA) gene cluster remained on 9p, whereas the IFNBI gene moved to 11q.…”

Section: Discussionmentioning

confidence: 99%

Mapping chromosome band 11q23 in human acute leukemia with biotinylated probes: identification of 11q23 translocation breakpoints with a yeast artificial chromosome.

Rowley

Dı́az

Espinosa

et al. 1990

208

101

“…Molecular studies of translocations in solid tumors lag far behind the study of leukemias due to the technical difficulties ofchromosome analysis in tissue samples (1,2). Human chromosome 11 contains several sites of chromosome rearrangement associated with tumors including t(11;22)(q13;q13) rearrangements involving the BCLI (breakpoint cluster 1) locus in B-cell chronic lymphocytic leukemia, B-cell non-Hodgkin lymphoma, and multiple myeloma (6), t(4;11)(q21;23) associated with infantile acute lymphoblastic leukemia (7), and t(9;11)(p22;q23) and t(11;19)(q23;pl3) in cases of acute monocytic leukemia (8). The t(11;22)(q24;q12) translocations of Ewing sarcoma (ES), peripheral neuroepithelioma (PNE), and Askin tumor appear to be cytogenetically identical and represent the best described and most consistent chromosome abnormalities associated with solid tumors (9,10).…”

Section: Introductionmentioning

confidence: 99%

Molecular localization of the t(11;22)(q24;q12) translocation of Ewing sarcoma by chromosomal in situ suppression hybridization.

Selleri

Hermanson

Eubanks

et al. 1991

Chromosome translocations are associated with a variety of human leukemias, lymphomas, and solid tumors. To localize molecular markers flanking the t(11;22) (q24;ql2) breakpoint that occurs in virtually all cases of Ewing sarcoma and peripheral neuroepithelioma, high-resolution chromosomal in situ suppression hybridization was carried out using a panel of cosmid clones localized and ordered on chromosome llq. The location of the Ewing sarcoma translocation breakpoint was determined relative to the nearest two cosmid markers on 11q, clones 23.2 and 5.8, through the analysis of metaphase chromosome hybridization. By in situ hybridization to interphase nuclei, the approximate physical separation of these two markers was determined. In

“…It is has been postulated that ETSJ, located at band 11q23, becomes deregulated as a result of these specific chromosome translocations. In the (9;11) translocation, the ETSI gene is translocated from chromosome 11 to the short arm of chromosome 9 adjacent to the IFN gene cluster (26). In the (4;11) translocation, the ETSJ gene was found to translocate from chromosome 11 to chromosome 4 (17).…”

Section: Discussionmentioning

confidence: 90%

“…In chronic myelogenous leukemia with the (9;22) translocation (Philadelphia chromosome) the BCR and ABL genes are fused resulting in the expression of a chimeric protein (22)(23)(24)(25). Abnormalities of chromosome 11 involving band q23 such as reciprocal translocations, deletions, inverted insertions, and homogenously staining regions have been associated with a variety of leukemias and lymphomas (17,26,27). The following three reciprocal translocations involving 11q23 have been described: t(4;11)(q21;q23); t(9;11)(p22;q23); and t(11;19)(q23;pl3).…”

Section: Discussionmentioning

confidence: 99%

Interferon-inducible gene maps to a chromosomal band associated with a (4;11) translocation in acute leukemia cells.

Luster¹,

Jhanwar²,

Chaganti³

et al. 1987

University of Minnesota, Minneapolis, MN 55455 9ommunicated by Lloyd J. Old, December 30, 1986 'AISTRACT An interferon-inducible cytokine, IP-10, containg homology to a family of proteins having chemotactic t-(platelet factor 4, f3-thromboglobulin) and mitogenic (connective tissue-activating peptide HI) activities has been mapped to chromosome 4 at band q21, a locus associated with an acute monocytic/B-lymphocyte lineage leukemia that exhibits the nonrandom translocation t(4;11)(q21;q23 METHODSChromosomal Localization by in Situ Hybridization. Meiotic and somatic chromosome preparations were prepared and hybridized with an IP-10 gene probe as described (10-12). The probe was a pUC plasmid containing a cDNA insert encoding the IP-10 protein (2), which contains the cap site (A.D.L., unpublished results) and two-thirds of the 3'-untranslated region. Plasmid DNA was labeled to high specific activity by nick-translation with [3H]deoxynucleotide triphosphates as described (10). The hybridized and washed slides were exposed for autoradiography (10) 2868The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.