Interfering with the high-affinity interaction between wheat amylase trypsin inhibitor CM3 and toll-like receptor 4: in silico and biosensor-based studies

Cuccioloni, Massimiliano; Mozzicafreddo, Matteo; Bonfili, Laura; Cecarini, Valentina; Giangrossi, Mara; Falconi, Maurizio; Saitoh, Shin-Ichiroh; Eleuteri, Anna Maria; Angeletti, Mauro

doi:10.1038/s41598-017-13709-1

Cited by 35 publications

(40 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…ATIs represent 2%-4% of total wheat protein and are believed to induce an innate immune response via activation of the Toll-like receptor 4 (TLR4) on immune cells within the intestine. They have also been shown to be involved in an adaptive immune response, have been implicated in coeliac disease, and may also contribute to inflammation in other disorders [48][49][50][51]. Additionally, legumes, which have a higher protein content than cereals, contain significant levels of anti-nutritional factors such as lectins and trypsin inhibitors, and two major storage proteins, vicilins and legumins, which are all resistant to proteolysis in the small intestine [46,52,53].…”

Section: Resistant Proteins-beyond Glutenmentioning

confidence: 99%

Reply to “A Gluten Reduction Is the Patients’ Choice for a Dietary ‘Bottom Up’ Approach in IBS—A Comment on “A 5Ad Dietary Protocol for Functional Bowel Disorders” Nutrients 2019, 11, 1938”

Ibrahim

Stribling

2020

Nutrients

View full text Add to dashboard Cite

show abstract

Section: Resistant Proteins-beyond Glutenmentioning

confidence: 99%

Reply to “A Gluten Reduction Is the Patients’ Choice for a Dietary ‘Bottom Up’ Approach in IBS—A Comment on “A 5Ad Dietary Protocol for Functional Bowel Disorders” Nutrients 2019, 11, 1938”

Ibrahim

Stribling

2020

Nutrients

View full text Add to dashboard Cite

show abstract

“…58 Os efeitos citotóxicos poderiam ser explicados pelos inibidores da alfa-amilase/tripsina do trigo, os quais poderiam ser o gatilho para a resposta imune inata, promovendo a liberação de citocinas por monócitos, macrófagos e células dendríticas. 25,59,[66][67][68][69] Segundo Volta et al, possíveis mecanismos fisiopatológicos da SNCG/T incluem ativação imune inata e adaptativa, barreira epitelial intestinal prejudicada e alterações no microbioma intestinal. Estes fatores interligados podem ser explorados por sua relevância clínica como possíveis biomarcadores.…”

Section: O Glútenunclassified

Sensibilidade não-celíaca ao glúten

Boarim¹

2018

International Journal of Nutrology

View full text Add to dashboard Cite

ResumoEntre as reações adversas a alimentos, os distúrbios relacionados ao glúten vêm atualmente ganhando cada vez mais espaço nas publicações científicas e não científicas. Estes distúrbios são divididos em três entidades: doença celíaca (DC), alergia ao trigo (AT) e sensibilidade não-celíaca ao glúten ou ao trigo (SNCG/T), considerando que este cereal vem sendo associado mais frequentemente às manifestações, discutindo-se ainda se não seria o real componente alimentar desencadeante. A DC é uma enteropatia autoimune, enquanto a AT é uma reação de hipersensibilidade, mediada ou não mediada pela imunoglobulina E (IgE). A SNCG/T é uma entidade emergente, de fisiopatologia ainda discutida, que vários pesquisadores propõem como diagnóstico de exclusão em relação à AT e à DC. A SNCG/T vem despertando grande celeuma popular e polêmica nos meios médicos. A presente revisão tem por objetivo abordar a SNCG/T, procurando elencar elementos de distinção dos outros dois distúrbios retrocitados discutindo aspectos como características clínicas, diagnóstico diferencial, fisiopatologia e tratamento. Em muitos trabalhos, salienta-se a possibilidade de sobreposição ou de confusão diagnóstica com outras doenças funcionais gastrointestinais, como síndrome do intestino irritável (SII) e oligossacarídeos, dissacarídeos, monossacarídeos e polióis fermentáveis (FODMAPs, na sigla em inglês). Concluímos que a SNGG/T, embora constitua uma entidade clínica reconhecida por um número expressivo de pesquisadores, ainda é objeto de amplo questionamento quanto à sua caracterização, ao seu diagnóstico e à sua fisiopatologia. Isto posto, considerando sua elevada prevalência estimada e as repercussões populares, indubitavelmente trata-se de assunto digno de estudos mais aprofundados e bem conduzidos.

show abstract

“…Although all the three proteins induced degranulation, the most effective one was CM3, accounting for the important role played by this protein in triggering allergic reactions. Moreover, CM3 has an important role in innate immune response, at least in monocytes, macrophages, and dendritic cells [12,13].…”

Section: Introductionmentioning

confidence: 99%

Reduction of Allergenic Potential in Bread Wheat RNAi Transgenic Lines Silenced for CM3, CM16 and 0.28 ATI Genes

Kalunke

Tundo

Sestili

et al. 2020

IJMS

View full text Add to dashboard Cite

Although wheat is used worldwide as a staple food, it can give rise to adverse reactions, for which the triggering factors have not been identified yet. These reactions can be caused mainly by kernel proteins, both gluten and non-gluten proteins. Among these latter proteins, α-amylase/trypsin inhibitors (ATI) are involved in baker’s asthma and realistically in Non Celiac Wheat Sensitivity (NCWS). In this paper, we report characterization of three transgenic lines obtained from the bread wheat cultivar Bobwhite silenced by RNAi in the three ATI genes CM3, CM16 and 0.28. We have obtained transgenic lines showing an effective decrease in the activity of target genes that, although showing a higher trypsin inhibition as a pleiotropic effect, generate a lower reaction when tested with sera of patients allergic to wheat, accounting for the important role of the three target proteins in wheat allergies. Finally, these lines show unintended differences in high molecular weight glutenin subunits (HMW-GS) accumulation, involved in technological performances, but do not show differences in terms of yield. The development of new genotypes accumulating a lower amount of proteins potentially or effectively involved in allergies to wheat and NCWS, not only offers the possibility to use them as a basis for the production of varieties with a lower impact on adverse reaction, but also to test if these proteins are actually implicated in those pathologies for which the triggering factor has not been established yet.

show abstract

Interfering with the high-affinity interaction between wheat amylase trypsin inhibitor CM3 and toll-like receptor 4: in silico and biosensor-based studies

Cited by 35 publications

References 62 publications

Reply to “A Gluten Reduction Is the Patients’ Choice for a Dietary ‘Bottom Up’ Approach in IBS—A Comment on “A 5Ad Dietary Protocol for Functional Bowel Disorders” Nutrients 2019, 11, 1938”

Reply to “A Gluten Reduction Is the Patients’ Choice for a Dietary ‘Bottom Up’ Approach in IBS—A Comment on “A 5Ad Dietary Protocol for Functional Bowel Disorders” Nutrients 2019, 11, 1938”

Sensibilidade não-celíaca ao glúten

Reduction of Allergenic Potential in Bread Wheat RNAi Transgenic Lines Silenced for CM3, CM16 and 0.28 ATI Genes

Contact Info

Product

Resources

About