Interferences of suspended clay fraction in protein quantitation by several determination methods

Lozzi, Irene; Pucci, Amaranta; Pantani, Ottorino‐Luca; D’Acqui, Luigi Paolo; Calamai, Luca

doi:10.1016/j.ab.2008.01.040

Cited by 13 publications

(6 citation statements)

References 15 publications

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“…Las cenizas empleadas en la prueba se obtuvieron por incineración de una muestra de lodo a 525 °C de acuerdo con el método Tappi T211. Todas las muestras fueron centrifugadas a 5300 rpm por 10 minutos a 4°C, con el fin de reducir las interferencias por las fracciones de arcilla suspendidas durante la cuantificación de proteínas según Lozzi et al (2008). El sobrenadante fue filtrado por membrana PTFE de 0.2 µm previo al análisis de proteínas según Bradford mediante el ensayo de microplatos con el equipo iMark Microplate -Reader marca Biorad.…”

Section: Adsorción De Celulasasunclassified

“…Los resultados del ensayo de adsorción del presente trabajo, no son concluyentes, con base en las afirmaciones de Jørgensen & Olsson (2006) respecto al tiempo de adsorción de las enzimas, se considera que el tiempo de contacto propuesto por no es representativo para el proceso. Así mismo, el efecto descrito por Lozzi et al (2008), referente a la interferencia que causan las arcillas en el análisis de proteínas por diferentes métodos, también se excluye como factor de error, considerando que el tamaño de partícula en la kaolinita empleada como principal recubrimiento del papel de escritura se encuentra entre 0.7 -2 µm (Biermann, 1996;Hui Chen et al, 2012), razón por la cual se filtraron las muestras con membrana de 0.2 µm.…”

Section:  Adsorción De Celulasasunclassified

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Microbial diversity of cellulose hydrolysis

Wilson¹

2011

Current Opinion in Microbiology

308

204

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Section: Adsorción De Celulasasunclassified

Section:  Adsorción De Celulasasunclassified

Microbial diversity of cellulose hydrolysis

Wilson¹

2011

Current Opinion in Microbiology

308

204

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“…The specific ALP activity was stated as nmol/μg protein/min. The protein contents of the lysates were determined by bicinchoninic acid (BCA) assay with a calibration curve obtained from bovine serum albumin (BSA) in the range of 0–1.2 mg/mL 28…”

Section: Experimental Methodsmentioning

confidence: 99%

Microstructure, microhardness, and biocompatibility characteristics of yttrium hydroxyapatite doped with fluoride

2010

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The current study focused on doping of hydroxyapatite (HA) with constant yttrium (Y(3+) ) and varying fluoride (F(-) ) compositions to investigate its microstructure, microhardness, and biocompatibility. HA was synthesized by precipitation method and sintered at 1100°C for 1 h. Y(3+) and F(-) ion dopings resulted in changes in densities. In x-ray diffraction analysis, no secondary phase formation was observed. Lattice parameters decreased upon ion substitutions. Scanning electron microscopy (SEM) results showed that ion addition resulted in smaller grains. In Fourier transform infrared spectroscopy analysis, F(-) ion substitution was confirmed. HA doped with 2.5% Y(3+) and 1% F(-) exhibited the highest microhardness. Y(3+) and F(-) ions improved Saos-2 cell proliferation on discs in Methylthiazolyldiphenyl-tetrazolium (MTT) assay. In SEM analysis, cells attached and proliferated on all disc surfaces. Alkaline phosphatase (ALP) assay showed that cell differentiation on the discs was improved by doping HA with an optimum F(-) amount. Dissolution tests revealed that structural stability of HA was improved with F(-) ion incorporation. The dissolution behavior of fluoridated samples exhibited a parallel pattern with the cell proliferation and differentiation behavior on these samples. Overall, this work shows that fluoride and yttrium cosubstitution into HA HA2.5Y1F was the most promising material for biomedical applications.

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“…However, in the application of membrane technology especially for MWCO determination, it was found that precipitation of PEG is an undesirable step and must be avoided since it could interfere during the reading of absorbance values by using UV spectrophotometer. The presence of precipitate causes spectral interferences due to the colloidal particles spread in the solution [27,28]. Therefore, in order to have an accurate absorbance reading, appropriate dilutions of the samples are necessarily required to make sure their concentrations are below 7.5 ppm before the addition of the two reagents (barium chloride and iodine) into the samples to avoid precipitation from occurs.…”

Section: (A) (B)mentioning

confidence: 99%

Molecular Weight Cut-Off Determination of Pressure Filtration Membranes via Colorimetric Detection Method

Yusoff

Rohani²,

Mohammad³

2017

MJAS

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Molecular weight cutoff (MWCO) of commercial and in-house fabricated membranes is obtained at 90% rejection of various types of solutes with different molecular weights (MWs). PEG quantification methods have been favorably employed to determine membranes' MWCO. Most of the quantification methods utilizing high-end equipment using high performance liquid chromatography (HPLC) and low-end colorimetric method. HPLC method requires an established chromatographic technique using specific diluents, columns and detectors while the most referred colorimetric method requires a few processing steps with concentration up to 7.5 ppm by using UV spectrophotometer. In this work, a newly modified colorimetric method was established to conduct a simple measurement for a membrane's MWCO. The newly modified method has an excellent linearity for the calibration curves which R 2 values are closed to 1 with concentration of up to 150 ppm. A MWCO determination test conducted using different commercial membranes for confirming this newly modified method found that similar MWCO values were obtained as given by the membrane' manufacturers. In conclusion, this newly modified method is simpler than the formerly used colorimetric method, reliable and applicable for determining the MWCO of membranes ranging from UF to NF at a higher range of PEG concentrations (>150 ppm).

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Interferences of suspended clay fraction in protein quantitation by several determination methods

Cited by 13 publications

References 15 publications

Microbial diversity of cellulose hydrolysis

Microbial diversity of cellulose hydrolysis

Microstructure, microhardness, and biocompatibility characteristics of yttrium hydroxyapatite doped with fluoride

Molecular Weight Cut-Off Determination of Pressure Filtration Membranes via Colorimetric Detection Method

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