Interference of the PAF-acether antagonist BN 52021 with passive anaphylaxis in the guinea-pig

Lagente, Vincent; Touvay, Caroline; Randon, Jacques; Desquand, S.; Cirino, Maria; Vilain, B.; Lefort, Jean; Braquet, P.; Vargaftig, B.B.

doi:10.1016/0090-6980(87)90011-6

Cited by 43 publications

(22 citation statements)

References 31 publications

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“…Broder et al [14] reported that 9 days are an opti mal delay between serum transfer and challenge with antigen. Under these conditions, it was expected that shock in animals transfused with serum for more than a week before the experiment should mostly involve IgE [15,16]. Nevertheless, in our hands, heating the serum, a procedure that inactivates IgE but not IgG antibodies [4,17], failed to reduce either the in vitro ability of serum to sensitise parenchymal lung strips or its ability to sensitise in vivo tissues within 9 days.…”

Section: Discussionmentioning

confidence: 51%

“…One alternative procedure is to use guinea pigs sensitised with serum from actively sensi tised animals and to trigger shock some time after the transfer of antibodies. This enables control of the amounts of serum administered to each animal [15,16]. Broder et al [14] reported that 9 days are an opti mal delay between serum transfer and challenge with antigen.…”

Section: Discussionmentioning

confidence: 99%

“…In most studies on passive anaphylaxis, guinea pigs sensitised with serum from actively immunised animals, containing thermolabile IgE and thermo stable IgG, are challenged after 7-14 days [14][15][16]. Under those conditions, it was expected that shock in animals transferred with serum for more than 1 week before the experiment mostly involves IgE [15,16].…”

Section: Introductionmentioning

confidence: 99%

“…Under those conditions, it was expected that shock in animals transferred with serum for more than 1 week before the experiment mostly involves IgE [15,16]. However, the role of IgG in those models might have been minimised, because of the homocytotropic properties of IgGl [4,17,18] and of the ability of IgG2 to induce the generation of anaphylatoxin [19][20][21] and since sensitisation following the adminis tration of anti-serum to ovalbumin (OA) heated for 4 h at 56 °C persists for 28-35 days in the guinea pig [22].…”

Section: Introductionmentioning

confidence: 99%

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Role of Immunoglobulins G1 and G2 in Anaphylactic Shock in the Guinea Pig

Desquand

Rothhut²,

Vargaftig³

1990

Int Arch Allergy Immunol

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Heating serum from actively sensitised guinea pigs did not remove its ability to sensitise recipient animals in vivo and parenchymal lung strips in vitro to anaphylaxis. Thermoresistant antibodies should thus account for the transferable sensitising effect, which persists for at least 9 days. IgG1 and IgG2, contained in the serum, were separated by affinity chromatography to determine the importance and the participation of these subclasses in passive anaphylactic shock. IgG1, present in smaller amounts than IgG2, was more effective in sensitising isolated lung strips. The intravenous administration of ovalbumin to guinea pigs, which had been injected with 0.8 mg/kg of IgG1 or 2 mg/kg of IgG2 9 days beforehand, induced an intense bronchoconstriction with leucopenia and moderate thrombopenia, suggesting an as yet undescribed role for IgG2 in passive tissue sensitisation. The use of mepyramine, an antagonist of the histamine H₁ receptor, WEB 2086, an antagonist of platelet-activating factor, and nordihydroguaiaretic acid, a dual inhibitor of cyclooxygenase and lipooxygenase, alone or associated, demonstrated that the anaphylactic contraction of lung strips from guinea pigs sensitised by IgG1 is mediated by histamine and arachidonate derivatives, whereas that of lung strips from guinea pigs sensitised with IgG2 is mostly mediated by histamine. In addition, the association of the three potential antagonists slightly reduced the anaphylactic contraction of lung strips provided by guinea pigs sensitised by serum. Our results, using a sensitisation procedure considered until now to involve exclusively IgE antibodies, indicate that IgG1 and IgG2 are in fact the essential antibodies for passive anaphylactic shock in the guinea pig.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of Immunoglobulins G1 and G2 in Anaphylactic Shock in the Guinea Pig

Desquand

Rothhut²,

Vargaftig³

1990

Int Arch Allergy Immunol

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“…BN 52021, which is not an antihistamine, was first reported to inhibit anaphylactic bronchoconstriction induced by the intravenous (i.v. ) or intra-pulmonary aerosolization of ovalbumin (OA) to guinea-pigs passively sensitized with homologous (Cirino et al, 1986;Vilain et al, 1986;Desquand et al, 1987;Lagente et al, 1987) or with heterologous (Touvay et al, 1985;1986b; serum raised in animals immunized with OA. Overall, these results suggested that PAF contributes to passive anaphylaxis and led us to study the effect of BN 52021 on bronchoconstriction induced by active anaphylaxis.…”

Section: Introductionmentioning

confidence: 99%

Interference of BN 52021, an antagonist of PAF, with different forms of active anaphylaxis in the guinea‐pig: importance of the booster injection

Desquand

Lefort

Dumarey

et al. 1991

British J Pharmacology

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1 BN 52021, an antagonist of platelet activating factor (PAF), was inactive against bronchoconstriction in guinea-pigs sensitized with low amounts of ovalbumin (OA) injected twice, at a 14 day interval and challenged i.v. 7 days later. 2 Serum IgG titers increased for 7 weeks after the booster injection at day 14 and returned to low levels at day 96. 3 Administered by the intratracheal (i.t.) route at 1 mg, BN 52021 failed to inhibit bronchoconstriction induced by the i.t. administration of OA to guinea-pigs tested 7, 28, 56 and 84 days after the booster injection, even when the titers of circulating IgG had declined with time. BN 52021 was also inactive against bronchoconstriction in guinea-pigs boosted at day 98 and tested 7 days later and against contractions and thromboxane (Tx) B2 and histamine release induced by OA-challenged parenchymal lung strips from the boosted guinea-pigs. 4 Sensitized unboosted guinea-pigs displayed reduced IgG serum titers. Used 21 or 70 days after the sensitizing injection, they did develop bronchoconstriction upon the i.t. instillation of OA, which was blocked by BN 52021. The latter also inhibited OA-induced contractions of lung parenchymal strips from these unboosted guinea-pigs. 5 When boosted and non-boosted guinea-pigs received OA i.t. and bronchoalveolar lavage fluid was collected 10 min later, the number of eosinophils increased markedly in boosted, but not in non-boosted guinea-pigs.6 The booster injection of antigen thus modifies the response of the lung and PAF appears to be relevant for antigen-induced bronchoconstriction in unboosted animals, but loses its major role following the booster injection.

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Natural antagonists of platelet‐activating factor

Hosford

Mencia‐Huerta

Page

et al. 1988

Phytotherapy Research

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Several groups of antagonists of platelet-activating factor (PAF) are reviewed. The characteristics of PAF binding sites on mammalian cell membranes are described together with the activities of non-specific and synthetic PAF-antagonists. The specificity of the ginkgolides to bind to PAF-receptors is discussed and the effects of these natural products in a range of biological systems is emphasized. Other groups of natural PAF-antagonists described are the lignans and gliotoxin related compounds. The structural chemistry, structure-activity relationships and pharmacological properties of these drugs are compared to the ginkgolides.

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Interference of the PAF-acether antagonist BN 52021 with passive anaphylaxis in the guinea-pig

Cited by 43 publications

References 31 publications

Role of Immunoglobulins G1 and G2 in Anaphylactic Shock in the Guinea Pig

Role of Immunoglobulins G1 and G2 in Anaphylactic Shock in the Guinea Pig

Interference of BN 52021, an antagonist of PAF, with different forms of active anaphylaxis in the guinea‐pig: importance of the booster injection

Natural antagonists of platelet‐activating factor

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