Interference of the Histone Deacetylase Inhibits Pollen Germination and Pollen Tube Growth in Picea wilsonii Mast

Cui, Yaning; Ling, Yu; Jun-hui, Zhou; Li, Xiaojuan

doi:10.1371/journal.pone.0145661

Cited by 13 publications

(9 citation statements)

References 42 publications

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“…Genes showing over 100-fold decreases in UI-competent pollen also included two Histone Deacetylases (HDACs, Sopen11g004040 and Sopen11g004050; Additional file 1 : Table S8). The proper function of HDACs has been linked to successful pollen tube germination and tip growth in Picea willsoni [ 83 ].…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic analysis links gene expression to unilateral pollen-pistil reproductive barriers

et al. 2017

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BackgroundUnilateral incompatibility (UI) is an asymmetric reproductive barrier that unidirectionally prevents gene flow between species and/or populations. UI is characterized by a compatible interaction between partners in one direction, but in the reciprocal cross fertilization fails, generally due to pollen tube rejection by the pistil. Although UI has long been observed in crosses between different species, the underlying molecular mechanisms are only beginning to be characterized. The wild tomato relative Solanum habrochaites provides a unique study system to investigate the molecular basis of this reproductive barrier, as populations within the species exhibit both interspecific and interpopulation UI. Here we utilized a transcriptomic approach to identify genes in both pollen and pistil tissues that may be key players in UI.ResultsWe confirmed UI at the pollen-pistil level between a self-incompatible population and a self-compatible population of S. habrochaites. A comparison of gene expression between pollinated styles exhibiting the incompatibility response and unpollinated controls revealed only a small number of differentially expressed transcripts. Many more differences in transcript profiles were identified between UI-competent versus UI-compromised reproductive tissues. A number of intriguing candidate genes were highly differentially expressed, including a putative pollen arabinogalactan protein, a stylar Kunitz family protease inhibitor, and a stylar peptide hormone Rapid ALkalinization Factor. Our data also provide transcriptomic evidence that fundamental processes including reactive oxygen species (ROS) signaling are likely key in UI pollen-pistil interactions between both populations and species.ConclusionsGene expression analysis of reproductive tissues allowed us to better understand the molecular basis of interpopulation incompatibility at the level of pollen-pistil interactions. Our transcriptomic analysis highlighted specific genes, including those in ROS signaling pathways that warrant further study in investigations of UI. To our knowledge, this is the first report to identify candidate genes involved in unilateral barriers between populations within a species.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-1032-4) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Transcriptomic analysis links gene expression to unilateral pollen-pistil reproductive barriers

et al. 2017

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“…Differences were not observed between this treatment and the control ( Fig 3A, 3B and 3E ), and similar results were found for the pollen tubes of Picea meyeri Rehd. Et Wils and Picea wilsonii Mast [ 9 , 10 ]. However, as the cell lysate concentration in the medium increased, the fluorescence became progressively weaker.…”

Section: Resultsmentioning

confidence: 99%

Rapid and Inexpensive Method of Loading Fluorescent Dye into Pollen Tubes and Root Hairs

Xing

et al. 2016

PLoS ONE

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The most direct technique for studying calcium, which is an essential element for pollen tube growth, is Ca2+ imaging. Because membranes are relatively impermeable, the loading of fluorescent Ca2+ probes into plant cells is a challenging task. Thus, we have developed a new method of loading fluo-4 acetoxymethyl ester into cells that uses a cell lysis solution to improve the introduction of this fluorescent dye into pollen tubes. Using this method, the loading times were reduced to 15 min. Furthermore, loading did not have to be performed at low (4°C) temperatures and was successful at room temperature, and pluronic F-127 was not required, which would theoretically allow for the loading of an unlimited number of cells. Moreover, the method can also be used to fluorescently stain root hairs.

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“…Genes showing 311 over 100-fold decreases in UI-competent pollen also included two Histone Deacetylases 312 (HDACs, Sopen11g004040 and Sopen11g004050; Additional File 1, Table S8). The proper function of HDACs has been linked to successful pollen tube germination and tip growth in 314 Picea willsoni [82]. 315 316 UI competence in additional S. habrochaites populations 317 Our initial analyses comparing transcriptomes of LA1777 and LA0407 reproductive 318 tissues led to some intriguing candidates that might be integral to UI.…”

Section: Differential Gene Expression Related To Ui-competence In Pollenmentioning

confidence: 99%

Linking gene expression to unilateral pollen-pistil reproductive barriers

Broz

Guerrero

Randle

et al. 2016

Preprint

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1Unilateral incompatibility (UI) is an asymmetric reproductive barrier that unidirectionally 2 prevents gene flow between species and/or populations. UI is characterized by a compatible 3 interaction between partners in one direction, but in the reciprocal cross fertilization fails, 4 generally due to pollen tube rejection by the pistil. Although UI has long been observed in 5 crosses between different species, the underlying molecular mechanisms are only beginning to 6 be characterized. The wild tomato relative Solanum habrochaites provides a unique study 7 system to investigate the molecular basis of this reproductive barrier, as populations within the 8 species exhibit both interspecific and interpopulation UI. Here we used a transcriptomic 9 approach to identify genes in both pollen and pistil tissues that may be probable key players in 10 UI. We confirmed UI at the pollen-pistil level between a self-incompatible population and a self-

show abstract

Interference of the Histone Deacetylase Inhibits Pollen Germination and Pollen Tube Growth in Picea wilsonii Mast

Cited by 13 publications

References 42 publications

Transcriptomic analysis links gene expression to unilateral pollen-pistil reproductive barriers

Transcriptomic analysis links gene expression to unilateral pollen-pistil reproductive barriers

Rapid and Inexpensive Method of Loading Fluorescent Dye into Pollen Tubes and Root Hairs

Linking gene expression to unilateral pollen-pistil reproductive barriers

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