Interference of single walled carbon nanotubes (SWCNT) in the measurement of lipid peroxidation in aquatic organisms through TBARS assay

Monserrat, José M.; Seixas, André L. R.; Ferreira-Cravo, Marlize; Bürguer-Mendonça, M.; Garcia, Solange Cristina; Kaufmann, Claudir Gabriel; Ventura‐Lima, Juliane

doi:10.1016/j.ecoenv.2017.02.034

Cited by 17 publications

(8 citation statements)

References 45 publications

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“…Exposure to SWCNT and MWCNT caused severe OS (102), inflammation, and histopathological alterations in various organs and cells, including the lungs (103), kidneys (15), liver (104), human skin HaCat cells (105), melanoma cells (106), lung cells (107), human microvascular endothelial cells (108), human embryonic kidney (HEK293) cells (15), human neuroblastoma cells (109), human mesothelial cells (110), and human keratinocytes (42). The increased contents of reactive oxygen species (ROS) and thiobarbituric acid reactive substance (TBARS), severe disruption in biomembranes, and disturbed mitochondrial redox state are well-described events involved in the mechanisms of CNT-mediated cytotoxicity (14,105,108,111). However, a few studies have focused on the OS-induced male subfertility in animals exposed to CNTs (45,87,94).…”

Section: Discussionmentioning

confidence: 99%

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

et al. 2020

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Carbon-based nanomaterials possess a remarkably high potential for biomedical applications due to their physical properties; however, their detrimental effects on reproduction are also concerned. Several reports indicate the toxicity of carbon nanotubes (CNT); nevertheless, their impact on intracellular organelles in the male reproductive organs has not been fully elucidated. Herein, we report on the reprotoxicity of single-walled (SWCNT) and multi-walled carbon nanotubes (MWCN) on several intracellular events and histological criteria in pubertal male BALB/c mice orally treated with 0, 10, and 50 mg/kg/day doses for 5 weeks. Biomarkers of oxidative stress and mitochondrial functionality, histopathological alterations, and epididymal sperm characteristics were determined. Oral administration of CNTs at 10 and 50 mg/kg evoked a significant decrement in weight coefficient, sperm viability and motility, hypo-osmotic swelling (HOS) test, sperm count, mitochondrial dehydrogenase activity, ATP content, total antioxidant capacity, and GSH/GSSH ratio in the testis and epididymal spermatozoa. On the other hand, percent abnormal sperm, testicular and sperm TBARS contents, protein carbonylation, ROS formation, oxidized glutathione level, and sperm mitochondrial depolarization were considerably increased. Significant histopathological and stereological alterations in the testis occurred in the groups challenged with CNTs. The current findings indicated that oxidative stress and mitochondrial impairment might substantially impact CNTs-induced reproductive system injury and sperm toxicity. The results can also be used to establish environmental standards for CNT consumption by mammals, produce new chemicals for controlling the rodent populations, and develop therapeutic approaches against CNTs-associated reproductive anomalies in the males exposed daily to these nanoparticles.

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Section: Discussionmentioning

confidence: 99%

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

et al. 2020

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“…For example, fullerenes are currently being used in cosmetic products, contrast agents, and in drug delivery 2,5–8 . The rapid growth of nanoparticle applications has led to increased production, raising concerns about environmental safety 9–12 . The effects of fullerenes on health and living organisms are a growing concern that has sparked studies of their interactions with biological membranes 13–21 .…”

Section: Introductionmentioning

confidence: 99%

Dependence of fullerene aggregation on lipid saturation due to a balance between entropy and enthalpy

Nalakarn

Boonnoy

Nisoh

et al. 2019

Sci Rep

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It is well-known that fullerenes aggregate inside lipid membranes and that increasing the concentration may lead to (lethal) membrane rupture. It is not known, however, how aggregation and rupture depend on the lipid type, what physical mechanisms control this behavior and what experimental signatures detect such changes in membranes. In this paper, we attempt to answer these questions with molecular simulations, and we show that aggregation and membrane damage depend critically on the degree of saturation of the lipid acyl chains: unsaturated bonds, or “kinks”, impose a subtle but crucial compartmentalization of the bilayer into core and surface regions leading to three distinct fullerene density maxima. In contrast, when the membrane has only fully saturated lipids, fullerenes prefer to be located close to the surface under the head groups until the concentration becomes too large and the fullerenes begin clustering. No clustering is observed in membranes with unsaturated lipids. The presence of “kinks” reverses the free energy balance; although the overall free energy profiles are similar, entropy is the dominant component in unsaturated bilayers whereas enthalpy controls the fully saturated ones. Fully saturated systems show two unique signatures: 1) membrane thickness behaves non-monotonously while the area per lipid increases monotonously. We propose this as a potential reason for the observations of low fullerene concentrations being effective against bacteria. 2) The fullerene-fullerene radial distribution function (RDF) shows splitting of the second peak indicating the emergence short-range order and the importance of the second-nearest neighbor interactions. Similar second peak splitting has been reported in metal glasses.

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“…Their formation is linked to high quantity of unsaturated fatty acids within samples, which underwent oxidation rapidly. 34 Presence of high microbial load could lead to enzymatic degradation, thus liberation of free fatty acids that are easily oxidized. 35 The increasing rate of TBARS values was lesser in the treated samples than the control throughout storage ( p < 0.05).…”

Section: Resultsmentioning

confidence: 99%

Betel (Piper betle L.) leaf ethanolic extracts dechlorophyllized using different methods: antioxidant and antibacterial activities, and application for shelf-life extension of Nile tilapia (Oreochromis niloticus) fillets

Tagrida

Benjakul

2021

RSC Adv.

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Interference of single walled carbon nanotubes (SWCNT) in the measurement of lipid peroxidation in aquatic organisms through TBARS assay

Cited by 17 publications

References 45 publications

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

Dependence of fullerene aggregation on lipid saturation due to a balance between entropy and enthalpy

Betel (Piper betle L.) leaf ethanolic extracts dechlorophyllized using different methods: antioxidant and antibacterial activities, and application for shelf-life extension of Nile tilapia (Oreochromis niloticus) fillets

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