Interference of ruthenium red analogues at photosystem II of spinach thylakoids

Garza-Ortiz, Ariadna; King-Dı́az, Beatriz; Sosa‐Torres, Martha E.; Lotina‐Hennsen, Blas

doi:10.1016/j.jphotobiol.2004.08.001

Cited by 4 publications

(7 citation statements)

References 33 publications

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“…Uncoupled PSII from H 2 O to DCPIP was measured through the reduction of DCPIP-supported O 2 evolutions, monitored polarographically. The reaction medium for assaying PSII activity was composed by the same basal electron-transport medium, but in the presence of 1 μM 2,5-dibromo-3-methyl-6-isopropyl-1,4- p -benzoquinone (DBMIB), 100 μM DCPIP, and 300 μM K 3 [Fe(CN) 6 ] and 6 mM NH 4 Cl [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Alkaloids Isolated from Ruta graveolens as Photosynthesis Inhibitors

et al. 2018

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Eight alkaloids (1–8) were isolated from Ruta graveolens, and their herbicide activities were evaluated through in vitro, semivivo, and in vivo assays. The most relevant results were observed for Compounds 5 and 6–8 at 150 μM, which decreased dry biomass by 20% and 23%, respectively. These are significant results since they presented similar values with the positive control, commercial herbicide 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). Based on the performed assays, Compound 5 (graveoline) is classified as an electron-transport inhibitor during the light phase of photosynthesis, as well as a plant-growth regulator. On the other hand, Compounds 6–8 inhibited electron and energy transfers, and are also plant-growth inhibitors. These phytotoxic behaviors based on acridone and quinolone alkaloids may serve as a valuable tool in the further development of a new class of herbicides since natural products represent an interesting alternative to replace commercial herbicides, potentially due their low toxicity.

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Section: Methodsmentioning

confidence: 99%

Evaluation of Alkaloids Isolated from Ruta graveolens as Photosynthesis Inhibitors

et al. 2018

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“…Chloroplasts Isolation and Chlorophyll Determination. Intact chloroplasts were isolated from market spinach leaves ( Spinaceae oleraceae L.) as previously published ( , ) and suspended in a medium that contained 400 mM sucrose, 5 mM MgCl 2 , 10 mM KCl, and 30 mM Tricine−KOH (pH 8.0). They were stored as concentrated suspensions in the dark for 1 h at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

“…PSII was measured from water to the reduction of DCPIP (dichlorophenol indophenol)-supported O 2 evolution. To perform this, 1 μM DBMIB (2,5-dibromo-3-methyl-6-isopropil- p -benzoquinone), 100 μM DCPIP, 500 μM K 3 [Fe(CN) 6 ], and 6 mM NH 4 Cl were added; MV was omitted ( , ).…”

Section: Methodsmentioning

confidence: 99%

Pachypodol from Croton ciliatoglanduliferus Ort. as Water-Splitting Enzyme Inhibitor on Thylakoids

González-Vázquez

Díaz

Aguilar

et al. 2006

J. Agric. Food Chem.

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A bioactivity-guided chemical study of aerial parts of Croton ciliatoglanduliferus Ort. led to the isolation for the first time of the flavonoids retusin (5-hydroxy-3,7,3',4'-tetramethoxyflavone) (1) and pachypodol (5,4'-dihydroxy-3,7,3'-trimethoxyflavone) (2) from the n-hexane extract. Compounds 1 and 2 were separated by preparative thin-layer chromatography. Compound 2 was the most active compound on ATP synthesis inhibition. The I50 value was 51 microM. Pachypodol behaves as a Hill reaction inhibitor. It inhibited the uncoupled electron flow on photosystem II partial reaction from water to dichlorophenol indophenol (DCPIP) and from water to sodium silicomolybdate. However, the uncoupled partial reaction from diphenylcarbazide to DCPIP and the uncoupled photosystem I from DCPIPred to MV were not inhibited by 2. These results were corroborated by fluorescence decay data. Therefore, pachypodol inhibits the water-splitting enzyme activity. Compound 1 with a 4'-methoxy group was a weak inhibitor, indicating that the 4' free -OH group is important for strong inhibition.

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“…Thus these PSII centers did not contribute to the observed downshift of the T m of the B-band. Alternatively, the above can be explained by a decreased energy requirement for the stabilization of charge pairs if RR directly interacted with the stromal side of PSII, as proposed by Garza-Ortiz et al (46).…”

Section: Discussionmentioning

confidence: 92%

“…In the present case, the loss of both Q-and B-bands emission shows that the S 2 state is not formed. It was suggested from fluorescence measurements that RR inhibition took place in both the donor and acceptor sides of PS II (46). Treatments of thylakoid membranes with RR resulted in a significant down-shift of the B-band (Fig.…”

Section: Discussionmentioning

confidence: 97%

Disorganization of the Mn₄Ca complex of photosystem II by ruthenium red: a thermoluminescence study

Gauthier

Carpentier

2008

Luminescence

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Ruthenium red (RR) is known to be an inhibitor that binds to Ca2+ sites. It releases Ca2+ and Cl(-) together with the extrinsic polypeptide of 17 kDa associated with the oxygen evolving complex of photosystem II. In this work we used thermoluminescence to study S2/3QB(-) and S2QA(-) charge recombination. It is shown that RR produced a deeper inhibition of oxygen evolution compared with the effect of extrinsic polypeptide or Ca2+/Cl(-) depletion. Even though Mn is not released, the Mn cluster is disorganized by RR and the S1-->S2 transition is inhibited.

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Interference of ruthenium red analogues at photosystem II of spinach thylakoids

Cited by 4 publications

References 33 publications

Evaluation of Alkaloids Isolated from Ruta graveolens as Photosynthesis Inhibitors

Evaluation of Alkaloids Isolated from Ruta graveolens as Photosynthesis Inhibitors

Pachypodol from Croton ciliatoglanduliferus Ort. as Water-Splitting Enzyme Inhibitor on Thylakoids

Disorganization of the Mn₄Ca complex of photosystem II by ruthenium red: a thermoluminescence study

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Interference of ruthenium red analogues at photosystem II of spinach thylakoids

Cited by 4 publications

References 33 publications

Evaluation of Alkaloids Isolated from Ruta graveolens as Photosynthesis Inhibitors

Evaluation of Alkaloids Isolated from Ruta graveolens as Photosynthesis Inhibitors

Pachypodol from Croton ciliatoglanduliferus Ort. as Water-Splitting Enzyme Inhibitor on Thylakoids

Disorganization of the Mn4Ca complex of photosystem II by ruthenium red: a thermoluminescence study

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Disorganization of the Mn₄Ca complex of photosystem II by ruthenium red: a thermoluminescence study