Interference of Peritoneal Dialysis Fluids with Cell Cycle Mechanisms

Büchel, Janine; Bartošová, Mária; Eich, Gwendolyn; Wittenberger, Timo; Klein-Hitpaß, Ludger; Steppan, Sonja; Hackert, Thilo; Schaefer, Franz; Passlick–Deetjen, Jutta; Schmitt, Claus Peter

doi:10.3747/pdi.2013.00010

Cited by 11 publications

(22 citation statements)

References 72 publications

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“…Whole exome expression analyses in human peritoneal mesothelial cells [ 9 ] demonstrated significant regulation of heme oxygenase-1 by PD fluids (p<0.000001) but of no other gene known to be involved in angiogenesis. In a separate set of experiments using new human peritoneal mesothelial cells lines from 4 different donors, quantification of heme oxygenase-1, angiopoietin-1, angiopoietin-2, VEGF-A, matrix metalloproteinase-1, fibroblast growth factor-2 and hypoxia-inducible factor-1a by qRT-PCR demonstrated no difference following LPDF and BPDF treatment, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…In vitro , lactate exerts significant mesothelial cell toxicity, even at neutral pH [ 8 ]. Bicarbonate buffered PD fluid with low glucose degradation product content induces less mesothelial gene regulation [ 9 ], less oncosis [ 10 ] and up-regulates aquaporin-1 dependent mesothelial cell migration [ 11 ]. In rats, pH adjusted lactate fluid induces more mesenterial angiogenesis than the respective bicarbonate buffered fluid, independent of the presence of glucose [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Bicarbonate buffered peritoneal dialysis fluid upregulates angiopoietin-1 and promotes vessel maturation

et al. 2017

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bicarbonate buffered peritoneal dialysis fluid upregulates angiopoietin-1 and promotes vessel maturation

et al. 2017

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“…Gotloib et al reported a higher degree of oxidative injury to the peritoneum. Büchel et al showed that icodextrin reduces the activity of mesothelial cells.…”

Section: Discussionmentioning

confidence: 86%

“…They showed an increment of P16 expression and β‐gal staining in mesothelial cells after stimulation with conventional icodextrin PDF. Büchel et al analyzed the effects of several PDFs on peritoneal cell cycle genes, and they showed that conventional icodextrin PDF stimulated P21 mRNA expression. From these results, we considered that conventional icodextrin might have inhibitory effects on cell growth and lead to cell senescence in mesothelial cells.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Neutral pH Icodextrin Peritoneal Dialysis Fluid on Mesothelial Cells

2018

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Conventional acidic icodextrin peritoneal dialysate (CI) has low biocompatibility due to its low pH, and a neutral pH icodextrin dialysate (NI) was developed. The influence of NI on the peritoneum has not been clarified. The effects of the two dialysates on cultured rat mesothelial cells were examined. CI, but not NI, increased α-smooth muscle actin, collagen type 1 and 3, and P21 mRNA expressions. CI with neutralized pH did not improve these harmful effects. With NI+ glucose degradation products (GDPs: same concentration as CI), mRNA expressions were comparable to those with NI alone. However, if NI + GDPs was acidified, mRNA levels matched those with CI. The proportion in the G2/M phase of the cell cycle was lower with CI than with NI stimulation. From these results, CI stimulated epithelial-mesenchymal transition, fibrotic changes, inhibited cell growth, and induced cell senescence. These effects were attributed to the combined low pH and high GDPs.

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Differential effects of biocompatible peritoneal dialysis fluids on human mesothelial and endothelial cells in 2D and 3D phenotypes

Jagirdar,

Pitaraki,

Rouka

et al. 2023

Artificial Organs

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IntroductionPeritoneal dialysis (PD) is a life maintaining treatment in patients with end‐stage renal disease. Its chronic application leads to peritoneal mesothelial layer denudation and fibrotic transformation along with vascular activation of inflammatory pathways. The impact of different PD fluids (PDF) on mesothelial and endothelial cell function and repair mechanisms are not comprehensively described.Materials and MethodsMesothelial (MeT‐5A) and endothelial cells (EA.hy926) were cultured in 1:1 ratio with cell medium and different PDF (icodextrin‐based, amino acid‐based, and glucose‐based). Cell adhesion, cell migration, and cell proliferation in 2D and spheroid formation and collagen gel contraction assays in 3D cell cultures were performed.ResultsCell proliferation and cell‐mediated gel contraction were both significantly decreased in all conditions. 3D spheroid formation was significantly reduced with icodextrin and amino acid PDF, but unchanged with glucose PDF. Adhesion was significantly increased by amino acid PDF in mesothelial cells and decreased by icodextrin and amino acid PDF in endothelial cells. Migration capacity was significantly decreased in mesothelial cells by all three PDF, while endothelial cells remained unaffected.ConclusionsIn 3D phenotypes the effects of PDF are more uniform in both mesothelial and endothelial cells, mitigating spheroid formation and gel contraction. On the contrary, effects on 2D phenotypes are more uniform in the icodextrin and amino acid PDF as opposed to glucose ones and affect mesothelial cells more variably. 2D and 3D comparative assessments of PDF effects on the main peritoneal membrane cell barriers, the mesothelial and endothelial, could provide useful translational information for PD studies.

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Interference of Peritoneal Dialysis Fluids with Cell Cycle Mechanisms

Cited by 11 publications

References 72 publications

Bicarbonate buffered peritoneal dialysis fluid upregulates angiopoietin-1 and promotes vessel maturation

Bicarbonate buffered peritoneal dialysis fluid upregulates angiopoietin-1 and promotes vessel maturation

Effect of Neutral pH Icodextrin Peritoneal Dialysis Fluid on Mesothelial Cells

Differential effects of biocompatible peritoneal dialysis fluids on human mesothelial and endothelial cells in 2D and 3D phenotypes

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