Interference of oleamide with analytical and bioassay results

Jug, Urška; Naumoska, Katerina; Metličar, Valentina; Schink, Anne; Makuc, Damjan; Vovk, Irena; Plavec, Janez; Lucas, Kurt

doi:10.1038/s41598-020-59093-1

Cited by 21 publications

(31 citation statements)

References 36 publications

(57 reference statements)

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“…Therefore, the plasma concentrations of oleamide may vary depending on physiological or pathophysiological conditions. On the other hand, oleamide could be leached into contact solvents from plastic labware and also non-plastic labware such as glass vial inserts (25) . Contamination of plastic and non-plastic labware-derived oleamide should be taken into consideration in the quantitative analysis of oleamide.…”

Section: Discussionmentioning

confidence: 99%

“…In healthy human plasma, stearamide is detected at concentrations of approximately 85-140 ng/ml (300-495 nM) (13) . As stearamide could also be leached into contact solvents from the plastic and non-plastic labwares (25) , their labwares should not be used for the preparation of plasma stearamide. We found that stearamide also activated mTOR signalling (data not shown).…”

Section: Discussionmentioning

confidence: 99%

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Oleamide rescues tibialis anterior muscle atrophy of mice housed in small cages

et al. 2020

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Skeletal muscle atrophy causes decreased physical activity and increased risk of metabolic diseases. We investigated the effects of oleamide (cis-9,10-octadecanamide) treatment on skeletal muscle health. The plasma concentration of endogenous oleamide was approximately 30 nM in the male ddY mice under normal physiological conditions. When the stable isotope-labelled oleamide was orally administered to male ddY mice (50 mg/kg), the plasma concentration of exogenous oleamide reached approximately 170 nM after 1 h. Male ddY mice were housed in small cages (one-sixth of normal size) to enforce sedentary behaviour and orally administered oleamide (50 mg/kg/day) for 4 weeks. Housing in small cages decreased tibialis anterior (TA) muscle mass and the cross-sectional area (CSA) of the myofibres in TA muscle. Dietary oleamide alleviated the decreases in TA muscle and resulted in plasma oleamide concentration of approximately 120 nM in mice housed in small cages. Housing in small cages had no influence on the phosphorylation levels of Akt, mTOR, and p70S6K in TA muscle; nevertheless, oleamide increased the phosphorylation levels of the proteins. Housing in small cages increased the expression of LC3-II and p62, but not LC3-I, in TA muscle, and oleamide reduced LC3-I, LC3-II, and p62 expression levels. In C2C12 myotubes, oleamide increased myotube diameter at ≥ 100 nM. Furthermore, the mTOR inhibitor, Torin 1, suppressed oleamide-induced increases in myotube diameter and protein synthesis. These results indicate that dietary oleamide rescued TA muscle atrophy in mice housed in small cages, possibly by activating the PI3K/Akt/mTOR signalling pathway and restoring autophagy flux.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Oleamide rescues tibialis anterior muscle atrophy of mice housed in small cages

et al. 2020

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“…Due to the fact that oleamide was found to be leaching from some plastic and other labware by our previous study [24], its presence in polymeric containers for food/beverages and medicines and its consequent leaching into the polymer contact liquid was assumed. Migration of oleamide from polymer starting materials used in food packing [9,[25][26][27], food storage containers [28,29] or plastic baby bottles [10] into some food simulants was already reported.…”

Section: Introductionmentioning

confidence: 99%

Oleamide, a Bioactive Compound, Unwittingly Introduced into the Human Body through Some Plastic Food/Beverages and Medicine Containers

Naumoska

Jug

Metličar

et al. 2020

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The purpose of the study was to investigate the migration of oleamide, a polymer lubricant, and a bioactive compound, from various plastic, marketed containers for food/beverages and medicines into polymer contact liquid. Methanol, food/medicine simulants or real samples were used to extract polymer leachables and extractables. Migrated oleamide into polymer contact liquids was determined by ultra-high performance liquid chromatography coupled to mass spectrometry (UHPLC-MS). The concentration of oleamide in the extracts of medicinal and insulin syringes was 7351 ng mL−1 and 21,984 ng mL−1, respectively. The leachates of intravenous (i.v.) infusion bottle, medicinal and insulin syringes contained 17 ng mL−1, 12 ng mL−1 and 152 ng mL−1, respectively. Oleamide in the extracts of dummies ranged from 30 to 39 ng mL−1, while in the leachates of baby bottles, from 12 to 23 ng mL−1. Leachates of soft drink bottles contained from 6 to 15 ng mL−1 oleamide, milk bottles from 3 to 9 ng mL−1, liquid yogurt bottles 17 ng mL−1 and water bottles from 11 to 18 ng mL−1. Bottled real matrices of oil and milk contained oleamide in the range from 217 to 293 ng mL−1. Moreover, the source of migrated oleamide (e.g., containers, caps, other parts) was identified. Oleamide is listed in the current EU regulations without a specific migration limit. Accordingly, these values are considered of no concern, unless future toxicological studies prove the opposite.

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“…One interesting compound is oleamide, a fatty-acid-amide that is present in the cerebrospinal fluid of sleepdeprived animals. Oleamide is also commonly used as a polymer lubricant and one of several bioactive contaminants known to leach from disposable laboratory plasticware (Jug et al, 2020;McDonald et al, 2008). Oleamide dose-dependently and stereospecifically potentiates GABA A and GlyRs (Coyne et al, 2002), and could have contributed to the GlyR effect reported by (Liu et al, 2010).…”

Section: Contaminantsmentioning

confidence: 99%

“…For drug targets like the GlyR, where zinc modulation occurs at concentrations of < 100 nM, a metal chelator such as EGTA, tricine, or DTPA (Kay, 2004;Paoletti et al, 1997) should be used. In addition, controls addressing contaminants that may arise from leaching labware and during sample handling/preparation (see (Jug et al, 2020;McDonald et al, 2008)) should be considered, and it may be appropriate to include details about how drug stocks were prepared and the composition and manufacturer of plastic and glass lab equipment used (including coverslips, storage tubes and drug delivery systems) in the methods section of the paper.…”

Section: Contaminantsmentioning

confidence: 99%