Interference Microscopy under Double-Wavelet Analysis: A New Approach to Studying Cell Dynamics

Sosnovtseva, Olga; Pavlov, Alexey N.; Brazhe, Nadezda A.; Brazhe, Alexey; Erokhova, Liudmila; Максимов, Г. В.; Mosekilde, Erik

doi:10.1103/physrevlett.94.218103

Cited by 38 publications

(33 citation statements)

References 19 publications

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“…The difference of the spectral structures for the examined cellular parts can be caused by the above-mentioned processes in the submembrane region. We should note that the spectral structure for leech neurons in general is very similar to that of snail neurons [41]. This points to a common origin of the spectral frequencies.…”

Section: Methodsmentioning

confidence: 58%

“…We have previously shown [41] that wavelet analysis reveals a range of frequencies in the refractive index dynamics of neurons. These frequencies result from the cooperative processes inside the cell.…”

Section: Methodsmentioning

confidence: 99%

“…Previously we found for neurons that rhythms between 0.1 and 0.3 Hz maintain constant values in time while rhythms between 1 and 2-4 Hz demonstrate variations caused by the slower components. In the high frequency range (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), there is a large number of coexisting rhythmic components with quite nonstationary behavior and different modulation properties [41].…”

Section: Modulation Processes Under Double-wavelet Analysismentioning

confidence: 99%

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Unraveling Cell Processes: Interference Imaging Interwoven with Data Analysis

Brazhe

Pavlov

et al. 2006

J Biol Phys

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The paper presents results on the application of interference microscopy and wavelet-analysis for cell visualization and studies of cell dynamics. We demonstrate that interference imaging of erythrocytes can reveal reorganization of the cytoskeleton and inhomogenity in the distribution of hemoglobin, and that interference imaging of neurons can show intracellular compartmentalization and submembrane structures. We investigate temporal and spatial variations of the refractive index for different cell types: isolated neurons, mast cells and erythrocytes. We show that the refractive dynamical properties differ from cell type to cell type and depend on the cellular compartment. Our results suggest that low frequency variations (0.1-0.6 Hz) result from plasma membrane processes and that higher frequency variations (20)(21)(22)(23)(24)(25)(26) are related to the movement of vesicles. Using double-wavelet analysis, we study the modulation of the 1 Hz rhythm in neurons and reveal its changes under depolarization and hyperpolarization of the plasma membrane. We conclude that interference microscopy combined with wavelet analysis is a useful technique for non-invasive cell studies, cell visualization, and investigation of plasma membrane properties.

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Section: Methodsmentioning

confidence: 58%

Section: Methodsmentioning

confidence: 99%

Section: Modulation Processes Under Double-wavelet Analysismentioning

confidence: 99%

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Unraveling Cell Processes: Interference Imaging Interwoven with Data Analysis

Brazhe

Pavlov

et al. 2006

J Biol Phys

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“…From this result we concluded that the rhythms at 0.8, 1.0 and 1.5 Hz are affected by the same processes, but the relative influence of each of these processes differs among the various rhythms. We should also note that the structure of the modulation spectra (both AM and FM) in the fibres was more complicated and had more components than the modulation spectra of similar rhythms in neurons (for comparison see Sosnovtseva et al 2005). From these observations we conclude that the rhythms at 0.8, 1.0 and 1.5 Hz in nerve fibres are likely to be influenced by more processes than in the case of neurons.…”

Section: Resultsmentioning

confidence: 73%

“…Measurement of the phase shifts resulting from the retardation of the propagating light with subsequent reconstruction of cellular phase images with submicrometre resolution is achieved in several types of interference microscopy: laser interference microscopy (Andreev & Indukaev 2003;Sosnovtseva et al 2005), digital holographic microscopy (Rappaz et al 2005), Fourier phase microscopy, Hilbert phase microscopy, diffraction phase microscopy, etc (Tychinskii 2001;Popescu et al 2004Popescu et al , 2005Popescu et al , 2006. These types of interference microscopy represent in some sense a replacement for the once quite popular technique of light scattering (Cohen et al 1968;Stepnoski et al 1991;Boppart 2003;Lazebnik et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Non-invasive study of nerve fibres using laser interference microscopy

Brazhe

Rodionova

et al. 2008

Phil. Trans. R. Soc. A.

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This paper presents the results of a laser interference microscopy study of the morphology and dynamical properties of myelinated nerve fibres. We describe the principles of operation of the phase-modulated laser interference microscope and show how this novel technique allows us to obtain information non-invasively about the internal structure of different regions of a nerve fibre. We also analyse the temporal variations in the internal optical properties in order to detect the rhythmic activity in the nerve fibre at different time scales and to shed light on the underlying biological processes. We observe pronounced frequencies in the dynamics of the optical properties and suggest that the oscillatory modes have similar origin in different regions, but different strengths and mutual modulation properties.

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