Interference in a chromogenic alpha-amylase assay caused by dye-labeled oligosaccharide-induced precipitation of lipoprotein.

Wickus, Gary G.; Dukerschein, R O; Pierce, J R; Davis, Kent D.

doi:10.1093/clinchem/28.10.2131

Cited by 6 publications

(1 citation statement)

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“…89 Explanations for this effect include optical interference of lipaemic serum during endpoint colorimetric methods, interaction between triglycerides and assay reagents and the presence of an amylase inhibitor in lipaemic serum. 90,91 Serial dilution or ultracentrifugation of lipaemic serum may be used to improve accuracy, although newer assays are significantly less susceptible to interference. 92–95 Rarely, very rapid normalization of serum amylase activity may arise from prompt disease resolution, profound early necrosis or accelerated renal clearance of amylase.…”

Section: Diagnosismentioning

confidence: 99%

Acute pancreatitis

Harper

Cheslyn-Curtis

2010

Ann Clin Biochem

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Acute pancreatitis (AP) is an important cause of morbidity and mortality worldwide and the annual incidence appears to be increasing. It presents as a mild self-limiting illness in 80% of patients. However, one-fifth of these develop a severe complicated life-threatening disease requiring intensive and prolonged therapeutic intervention. Alcohol and gallstone disease remain the commonest causes of AP but metabolic abnormalities, obesity and genetic susceptibility are thought be increasingly important aetiological factors. The prompt diagnosis of AP and stratification of disease severity is essential in directing rapid delivery of appropriate therapeutic measures. In this review, the range of diagnostic and prognostic assays, severity scoring systems and radiological investigations used in current clinical practice are described, highlighting their strengths and weaknesses. Increased understanding of the complex pathophysiology of AP has generated an array of new potential diagnostic assays and these are discussed. The multidisciplinary approach to management of severe pancreatitis is outlined, including areas of controversy and novel treatments.

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