Interference-free glucose sensor based on glucose-oxidase immobilized in an overoxidized non-conducting polypyrrole film

Centonze, Diego; Guerrieri, Antonio; Malitesta, Cosimino; Palmisano, Francesco; Zambonin, P. G.

doi:10.1007/bf00321865

Cited by 99 publications

(57 citation statements)

References 12 publications

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“…The resulting glucose sensor displayed a number of distinctive features, including the ability to effectively reject ascorbate, urate, cysteine, and acetaminophen [7]. A PPD film has also been employed [8,9] to protect from interferents a glucose sensor based on GOx chemically bonded to the surface of a reticulated vitreous carbon electrode.…”

Section: Introductionmentioning

confidence: 99%

Permeation of solutes through an electropolymerized ultrathin poly‐o‐phenylenediamine film used as an enzyme‐entrapping membrane

et al. 1994

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Permeation of electroactive organic probes through an electroinactive and passivating poly-ophenylenediamine (PPD) film electropolymerized on Pt and glassy carbon (GC) electrodes has been investigated by cyclic and rotating disk electrode (RDE) voltammetry. The access of solutes to the metal-polymer interface appears mainly governed by specific chemical interactions, influencing partition, and diffusion phenomena, rather than by exclusion effects based on molecular size or charge. Potential cycling of the film induces fine modifications in the chemical/physical structure of the polymer, as evidenced by electron spectroscopy for chemical analysis (ESCA) measurements and by an enhanced permeation of certain solutes. The membrane is, however, stable in the pH and potential range usually employed in its application, that is, as an enzyme-entrapping membrane in amperometric biosensors; because of membrane permselectivity, the electrochemical response of the most common electroactive interferents is deeply depressed.

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Section: Introductionmentioning

confidence: 99%

Permeation of solutes through an electropolymerized ultrathin poly‐o‐phenylenediamine film used as an enzyme‐entrapping membrane

et al. 1994

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“…The following enzymes have been used for biosensor preparation: cholesterol oxidase, EC 1.1.3.6, from Streptomyces species (18 …”

Section: Reagent$ Materials and Instrumentationmentioning

confidence: 99%

“…All of these methods provide simple one-step procedures for biosensor production. In the case of non-conducting polymers, such as poly-(0-phenylenediamine) [27][28][29][30] or 754 Trettnak el al. polypyrrole in its so-called "over-oxidized'' state [ 18,201, the polymer layer provides at the same time a membrane, which is capable of interference rejection, a fact that is of special importance for sensors based on hydrogen peroxide detection.…”

Section: Introductionmentioning

confidence: 99%

Cholesterol biosensors prepared by electropolymerization of pyrrole

1993

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Biosensors for cholesterol were prepared by electropolymerization of pyrrole in solutions of 0.1 M phosphate buffer containing 10 mM NaClO, and 15 to 55 IU/mL of cholesterol oxidase at a potential of 800 mV versus a calomel reference electrode (3 M KCl). The sensors were tested for their response toward cholesterol in batch. flow-through, and flow-injection systems by detection of the hydrogen peroxide formed in the enzymatic reaction. Flow-injection analysis (FIA) measurements with an injection loop of 50 pL were performed with a high reproducibility in the range of 0 to 250 pM cholesterol with solutions of constant detergent concentration (1% Triton X-100 in 0.1 M phosphate buffer of pH 7.0) and at a flow rate of 0.2 mL/min. At this flow rate, the detection limit was approximately 5 p M cholesterol and the sample throughput was 25 injections per hour. The response of the sensors toward ascorbic and uric acid was investigated.

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“…The differences in behavior niger and D-(/) glucose were supplied from Sigma Chemical Company (St. Louis, MO). The of these two electrodes depicted in the Figure 1 show that polymer has been affected by the enzyme imglucose solutions were allowed to mutarotate at room temperature for 24 …”

Section: Introductionmentioning

confidence: 99%

“…1 cose oxidase (GOx) by the electropolymerization Among these conducting or nonconducting of 4-methoxyphenol in the presence of GOx in the polymeric films, polypyrrole, 2-14 polyphenylenediaqueous media. Also, the effects of various paramamines, [15][16] polyindole, 17 poly(vinyl alcohol), 18 eters in both electropolymerization and measurepoly(vinyl alcohol) -butyl acrylate, [19][20] polythioment stages on the steady-state amperometric phene, 21 and polyphenylenes [22][23][24][25] have been widely response to glucose were systematically inused as entrapment media for the enzymes.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical synthesis and optimization of poly(4-methoxyphenol) film as a sensor material

zden

nci

Karagzler

1998

J. Appl. Polym. Sci.

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This article describes that electrochemical polymerization of 4-methoxyphenol in the presence of enzyme glucose oxidase produces adherent polymeric films containing the active enzyme onto the surface of platinum electrodes. Polymeric electrodes prepared in this one-step procedure can be operated for the glucose determination. The effects of the electrochemical polymerization parameters (for example, concentrations of monomer, electrolyte, and enzyme; film thickness; and polymerization potential) on the electrode preparation and the effects of amperometric measurement parameters (for example, pH, temperature) on the amperometric responses to the glucose of the prepared electrodes were systematically investigated, and optimal values were determined. Furthermore, glucose specificity and storage stability of the enzyme electrode were investigated.

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Interference-free glucose sensor based on glucose-oxidase immobilized in an overoxidized non-conducting polypyrrole film

Cited by 99 publications

References 12 publications

Permeation of solutes through an electropolymerized ultrathin poly‐o‐phenylenediamine film used as an enzyme‐entrapping membrane

Permeation of solutes through an electropolymerized ultrathin poly‐o‐phenylenediamine film used as an enzyme‐entrapping membrane

Cholesterol biosensors prepared by electropolymerization of pyrrole

Electrochemical synthesis and optimization of poly(4-methoxyphenol) film as a sensor material

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