Interference caused by the contents of serum separator tubes in the                Vitros CRP assay

Chang, Cheng-Jong; Lü, Jinying; Chien, Tzu-I; Kao, Jau-Tsuen; Lin, Mei-Chun; Shih, Pei-Chun; Yan, Shiao-Ni

doi:10.1258/000456303321610556

Cited by 17 publications

(11 citation statements)

References 6 publications

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“…Therefore, these laboratories are vulnerable to occlusion of sample probes from inappropriately separated blood samples. The partial or complete obstruction of sample probes, gel aspiration and/or inadequate sample aspiration by laboratory instrumentation may affect test results, increase turn-around time, and the cost of analyses (4,5,7). …”

Section: Discussionmentioning

confidence: 99%

Abnormal gel flotation in a patient with apperant pneumonia diagnosis: a case report

et al. 2014

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Introduction:Serum blood collection tubes with separator gel are widely used by many laboratories for chemistry analyses. We describe a case of a primary blood collection tube filled with blood sample and a floating separator gel.Materials and methods:The blood sample was collected from a 51 years old female in intensive care unit with the diagnosis of pneumonia into a BD Vacutainer SST tube (Becton Dickinson, NJ, USA) containing serum separator gel and conveyed to the core laboratory of Marmara University Hospital within 30 minutes from collection. Sample was immediately centrifuged at room temperature at 1500 × g for 10 minutes.Results:The analyses revealed a highly increased total protein concentration of 145 g/L (reference interval 64–83 g/L). The nephelometric analyses showed an elevated serum IgG concentration of 108 g/L (reference interval 6.5–16 g/L) and IgG lambda monoclonal band was determined by serum immunofixation electrophoresis.Conclusion:Limitation of the separator gel tubes in patients with a high plasma density and its possible effects on test results and laboratory costs should be remembered. The clinical diagnosis stated in the information system should also reveal known comorbid conditions besides the apparent admission reason. This information would avoid resampling, additional testing, and communication efforts with the clinicians.

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Section: Discussionmentioning

confidence: 99%

Abnormal gel flotation in a patient with apperant pneumonia diagnosis: a case report

et al. 2014

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“…However, some limitations associated with gel tubes. One limitation was that the gel contained within serum separator tubes had the potential for adsorbing analytes of interest (8,9) or releasing material that may interfere with analytes (10). Another limitation of serum separator tubes was that certain analytes, such as therapeutic drugs and steroid hormones, may be instable when serum were stored on the barrier for long periods of time (2,3).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of BD Vacutainer SST™ II plus tubes for special proteins testing

Li¹,

Yan²,

Yan³

et al. 2011

J. Clin. Lab. Anal.

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In the past few years, because of providing a closed system that allowed for collection, transport, processing, sampling, and storage of specimens, serum separator tubes gained widespread acceptance gradually in China. However, some limitations associated with gel tubes had been observed, for example, gel and analyte stability. In order to circumvent these problems, a new tube (BD Vacutainer(®) SST™ II Plus (BD SST™ II Plus)) containing a new gel was released by BD with respect to analyte and gel stability. We investigated the performance of BD SST™ II Plus tubes for special proteins testing using BD Vacutainer(®) Serum Glass Tubes (BD Serum Glass) as controls.Equivalence between these two types of tubes was demonstrated for all analytes at initial time, and data for all analytes except complement 3 (C3) and complement 4 (C4) indicated comparable stability over time in these two types of tubes. Concentration of C3 and C4 tended to increase with preservation time up to 72 hr in BD Serum Glass tubes. The stability of C3 and C4 was better in BD SST™ II Plus, which was demonstrated at timepoints up to 48 hr. We conclude that BD SST™ II Plus was suitable for collection and storage of samples for special proteins testing.

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“…Also, analyte stability in serum separator tubes had been documented in the past (7,8). One disadvantage of gel separator tubes was that the gel contained within such tubes had the potential for adsorbing analytes of interest (9,10) or releasing material that may interfere with assay chemistries (11). Another disadvantage of gel separator tubes was the instability of certain analytes when serum was stored for long periods of time on the barrier (2,3), such as therapeutic drugs and steroid hormones.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of BD vacutainer SST^™ II plus tubes for common tumor marker tests by Roche Diagnostics Modular E 170 analyzer

Feng

Yan

et al. 2010

Clinical Laboratory Analysis

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Serum separator tubes were introduced 35 years ago and were widely used in the clinical laboratory in China for routine collection of blood because of providing a closed system that allowed for collection, transport, processing, sampling, and storage of specimens. This type of tubes facilitated rapid separation of serum from cellular constituents of blood and also prevented hemolysis upon prolonged storage. However, there were some limitations associated with gel tubes (i.e., gel and analyte stability). In order to circumvent these problems, BD released a new serum separator tube containing a new gel (BD SST(™) II Plus). We investigated the performance of BD SST(™) II Plus tubes for tumor marker tests using BD Serum Glass tubes as controls. Equivalence between the BD SST(™) II Plus and BD Serum Glass tubes was demonstrated for all analytes at initial time. Also, all analytes remained stable when stored in BD SST(™) II Plus tubes up to 72 hr. Concentration of neuron-specific enolase tended to increase with preservation time up to 72 hr in BD Serum Glass tubes. We conclude that BD SST(™) II Plus was suitable for collection of blood and storage of serum for tumor marker tests.

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Interference caused by the contents of serum separator tubes in the Vitros CRP assay

Abstract: Background We have observed discrepancies between C-reactive protein (CRP) measured in serum prepared from a serum separator tube (SST) and that obtained from a plain tube, when using the Vitros CRP assay. Our study aimed at elucidating the cause of these discrepancies.

Cited by 17 publications

References 6 publications

Abnormal gel flotation in a patient with apperant pneumonia diagnosis: a case report

Abnormal gel flotation in a patient with apperant pneumonia diagnosis: a case report

Evaluation of BD Vacutainer SST™ II plus tubes for special proteins testing

Evaluation of BD vacutainer SST^™ II plus tubes for common tumor marker tests by Roche Diagnostics Modular E 170 analyzer

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Interference caused by the contents of serum separator tubes in the Vitros CRP assay

Abstract: Background We have observed discrepancies between C-reactive protein (CRP) measured in serum prepared from a serum separator tube (SST) and that obtained from a plain tube, when using the Vitros CRP assay. Our study aimed at elucidating the cause of these discrepancies.

Cited by 17 publications

References 6 publications

Abnormal gel flotation in a patient with apperant pneumonia diagnosis: a case report

Abnormal gel flotation in a patient with apperant pneumonia diagnosis: a case report

Evaluation of BD Vacutainer SST™ II plus tubes for special proteins testing

Evaluation of BD vacutainer SST™ II plus tubes for common tumor marker tests by Roche Diagnostics Modular E 170 analyzer

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Product

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Evaluation of BD vacutainer SST^™ II plus tubes for common tumor marker tests by Roche Diagnostics Modular E 170 analyzer