Interference by DOPAC and ascorbate during attempts to measure drug-induced changes in neostriatal dopamine with Nafion-coated, carbon-fiber electrodes

Wiedemann, Donna J.; Basse-Tomusk, Allison; Wilson, Robert L.; Rebec, George V.; Wightman, R. Mark

doi:10.1016/0165-0270(90)90089-x

Cited by 46 publications

(35 citation statements)

References 50 publications

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“…Table 1 were calibrated using CFEs in CV mode. In agreement with previous reports (Wiedemann et al, 1990;Pihel et al, 1996), CV was differentially sensitive to various catechol metabolites. At the same concentrations, DA, EP, and NE yielded 10 -20 times higher oxidation peaks than DOPAC, DOPEG, HVA, vanillylmandelic acid, or L-DOPA (Fig.…”

Section: Ipe In CV Modesupporting

confidence: 82%

Intracellular Patch Electrochemistry: Regulation of Cytosolic Catecholamines in Chromaffin Cells

et al. 2003

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Alterations in the cytosolic pool directly affect neurotransmitter synthesis and release and are suggested to be key factors in various neurodegenerative disorders. Although this cytosolic pool is the most metabolically active, it is miniscule compared with the amount of vesicular transmitter and has never been quantified separately. Here, we introduce intracellular patch electrochemistry (IPE), a technique that for the first time provides direct measurements of cytosolic oxidizable molecules in single mammalian cells. In amperometric mode, IPE detects total catechols, whereas in cyclic voltammetric mode, it preferentially measures catecholamines. In cultured chromaffin cells, the total cytosolic catechol concentration was 50 -500 M, of which ϳ10% were catecholamines. Reserpine, a vesicular monoamine transporter inhibitor, had no effect on the catecholamine pool but increased total catechols by fourfold to fivefold. Combined with pargyline, a monoamine oxidase inhibitor, reserpine increased catecholamine levels in the cytosol by approximately sixfold. Amphetamine induced a transient approximately fivefold accumulation of cytosolic catecholamines and a slow increase of total catechols. In cells incubated with 3,4-dihydroxy-L-phenylalanine (L-DOPA), catecholamines increased by ϳ2.5-fold and total catechols increased by approximately fourfold. Cytosolic catecholamines returned to control levels Յ10 min after L-DOPA withdrawal, whereas total catechols remained approximately twofold elevated even after a 1.5 hr incubation in L-DOPA-free media. Our data indicate that cytosolic catecholamines are strictly maintained at a defined level, and drug-induced increases in their concentrations lead to the accumulation of other catecholamine derivatives, such as DOPAC and 3,4-dihydroxyphenylethyleneglycol. These derivatives reside in the cytosol for hours after treatment and may be an underlying cause of drug-related cytotoxicity.

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Section: Ipe In CV Modesupporting

confidence: 82%

Intracellular Patch Electrochemistry: Regulation of Cytosolic Catecholamines in Chromaffin Cells

et al. 2003

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“…Detection of DA by in vivo voltammetry is complicated by the presence of electrochemically active compounds contributing to faradaic current (Wiedemann et al, 1990). The responses of 7 and 32 m CFEs to ascorbic acid at 400 M concentration were similar in shape as shown in Fig.…”

Section: Selectivity Of 32 M Cfe For Dopamine Versus Interfering Compmentioning

confidence: 70%

Characterization of a 32μm diameter carbon fiber electrode for in vivo fast-scan cyclic voltammetry

Chadchankar

Yavich

2012

Journal of Neuroscience Methods

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“…Alternatively, several research groups have employed the application of various layers of protective membranes on bare electrodes to reduce electrode fouling. These membranes include Nafion [15], cellulose acetate [16], agarose [17] and poly(4-vinylpyridine) [18]. However, many of these membrane materials suffer from drawbacks such as non-uniform thickness, lack of longevity, stability and fast response time [12].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of hydrogenated physically small carbon electrodes in resisting fouling during voltammetric detection of dopamine

Alwarappan

Butcher

Wong

2007

Sensors and Actuators B: Chemical

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Interference by DOPAC and ascorbate during attempts to measure drug-induced changes in neostriatal dopamine with Nafion-coated, carbon-fiber electrodes

Cited by 46 publications

References 50 publications

Intracellular Patch Electrochemistry: Regulation of Cytosolic Catecholamines in Chromaffin Cells

Intracellular Patch Electrochemistry: Regulation of Cytosolic Catecholamines in Chromaffin Cells

Characterization of a 32μm diameter carbon fiber electrode for in vivo fast-scan cyclic voltammetry

Evaluation of hydrogenated physically small carbon electrodes in resisting fouling during voltammetric detection of dopamine

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