Interference between p53 and cdc25C in cell cycle regulation

Abstract

Abstract. The eukaryotic cell cycle is regulated by a network of different protein kinases and phosphatases which are by various mechanisms linked to the growth suppressor p53. Cell cycle regulation is quite similar from yeast to man. Although there is no endogenous p53 in yeast expression of human p53 led to growth arrest of yeast cells which can be suppressed by simultaneous overexpression of cdc25C, a phosphatase regulating entry into mitosis. Herein, we show that overexpression of cdc25C in mammalian cells is insufficient in suppressing a p53 induced growth arrest. We further show that p53 is co-immunoprecipitated with cdc25C and p53 inhibits the cdc25C phosphatase activity in a dose-dependent manner. Thus, our data show that p53 like other binding partners of cdc25C, regulates entry into mitosis by binding to cdc25C.

Induction of G2/M Arrest by Berberine via Activation of PI3K/Akt and p38 in Human Chondrosarcoma Cell Line

“…Cdc25c controls progression through the G 2 phase and entry into mitosis. In the late G 2 phase, the cdc25c phosphatase dephosphorylates the cyclin B1/cdc2 complex leading to the activation of the cyclin B1/cdc2 complex and to entry into mitosis ( 5 ).…”

Induction of Cell Cycle Arrest in MKN45 Cells after Schiff Base Oxovanadium Complex Treatment Using Changes in Gene Expression of CdC25 and P53

“…Phosphatase CDC25 includes CDC25 A, CDC25 B, and CDC25 C, which are regulatory components in multiple cell cycle stages and activate different types of cyclin-dependent kinases (CDKs) [29]. Inhibition of CDC25 is an anti-cancer strategy.…”

Expressing and functional analysis of mammalian apoptotic regulators in yeast

“…These early studies seem to have uncovered a bona fide effect of p53 because a recent study has reported that p53 regulates cell cycle progression in mammalian cells by interacting with cdc25. 76 Although it is quite clear that there is no direct orthologue of p53 in the yeast genomes, these and various other studies 77 suggest that a functional homologue to p53 is likely to exist in yeast. Although alternative explanations exist, the ability of these heterologous human apoptotic regulators to function in yeast is largely taken as evidence for the underlying similarities in the regulation of apoptosis in yeast and mammalian cells.…”