Interference between M13 and oriM13 plasmids is mediated by a replication enhancer sequence near the viral strand origin

Johnston, Sean

doi:10.1016/0022-2836(84)90044-5

Cited by 35 publications

(27 citation statements)

References 21 publications

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“…1, red coloured region), which is absolutely required for both initiation and termination, and which is the necessary and sufficient sequence for the nicking by gpII to occur in vitro. The right-most (3 0 proximal on the plus strand) sequence of about 100 base pairs is rich in AT and constitutes the replication enhancer (Johnston & Ray 1984;Dotto et al 1984; see Fig. 1, yellow coloured region) which is dispensable to some extent.…”

Section: Plus Strand Originmentioning

confidence: 99%

Initiation mechanisms in replication of filamentous phage DNA

Horiuchi

1997

Genes to Cells

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Filamentous phage DNA replication occurs in two steps. First, an RNA-primed minus strand is made on the plus strand. Then, a new plus strand is made on the resulting double strand. The RNA primer which initiates synthesis of the minus strand is produced at a specific site on the plus strand template by the host RNA polymerase holoenzyme. Despite a lack of sequences similar to the promoter consensus, the DNA replication origin has a much higher affinity for the holoenzyme than the transcriptional promoters. The non-template strand of the single-stranded ¹10 region of the origin appears to be responsible for this high affinity. The recognition mechanisms seem to share common features with those in transcriptional promoters. Plus-strand synthesis is initiated by a specific nick introduced into the negatively supercoiled replicative form by the phage-encoded initiator protein. The nicking reaction is preceded by an ordered series of protein-induced DNA conformational changes.

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Section: Plus Strand Originmentioning

confidence: 99%

Initiation mechanisms in replication of filamentous phage DNA

Horiuchi

1997

Genes to Cells

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“…Insertion and deletion mutagenesis, followed by in vivo and in vitro assays performed in the laboratories of Zinder [99,[106][107][108][109][110] and Ray [54,100,101,111] have divided the origin region into two domains: domain A, approx. 40 nucleotides long, and domain B or the enhancer sequence, which is approx.…”

Section: Iv[' Initiation and Termination Signals Of Viral Strand Synmentioning

confidence: 99%

“…40 nucleotides long, and domain B or the enhancer sequence, which is approx. 100 nucleotides long [110,111] (Fig. 10).…”

Section: Iv[' Initiation and Termination Signals Of Viral Strand Synmentioning

confidence: 99%

“…100 nucleotides and is required only for plus-strand initiation. Disruption of this domain on an ori-plasmid by insertions and/or deletions at the AuaI site at position 5825 or the HaelII site at position 5867 reduces the biological activity to the level of domain A alone [100,101,110,111]. However, filamentous phage particles have been constructed and isolated which bypass the need of domain B for their replication.…”

Section: Iv[' Initiation and Termination Signals Of Viral Strand Synmentioning

confidence: 99%

“…These phages (e.g., R218, R330 and Mpl and their derivatives) need for their replication only the core of the functional origin (domain A), while domain B is totally dispensable [112,113]. Analysis of these phages showed that they had acquired, besides the original insertion in domain B at map position 5867 Deletions at the unique AvaI site on position 5825 of bacteriophage M13G ori 6 also have been constructed [111]. The deletions ranged from two nucleotides up to 37 nucleotides.…”

Section: Iv[' Initiation and Termination Signals Of Viral Strand Synmentioning

confidence: 99%

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