Interference between estradiol and L-triiodothyronine in the control of the proliferation of a pituitary tumor cell line

Zhou-Li, Fei; Skalli, Mourad; Albaladejo, V.; Joly-Pharaboz, Marie-Odile; Nicolas, Brigitte; André, Jean

doi:10.1016/0960-0760(93)90342-t

Cited by 8 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using in situ (30) and slot-blot hybridizations (5), we also found that T3 attenuated the EB-induced increase in PPE mRNA in the hypothalamus of OVX rats. These results suggest that TRs have the ability to compete with ER on estrogen-responsive genes in the rat hypothalamus, as previously reported in in vitro systems (2)(3)(4) (1996) Proc. Natl.…”

Section: Resultssupporting

confidence: 70%

“…Using slot-blot hybridization, we also found that ovariectomized (OVX) rats treated with high doses of triiodothyronine (T3) in combination with estradiol benzoate (EB) had significantly less PPE mRNA in the hypothalamus compared with females treated with EB alone. Taken together with data from other systems (1)(2)(3)(4), our molecular results suggest that there may be competition between TR and ER for hormone response element binding of hypothalamic genes, such as PPE.…”

mentioning

confidence: 69%

“…Manipulations of thyroid hormones and estrogen in vitro potentiate or mutually inhibit effects on gene expression (2)(3)(4). Our data presented in the accompanying paper (5) demonstrates that both TRs and ERs are present in rat hypothalamic nuclear extracts and both can bind to a preproenkephalin (PPE) promoter ERE.…”

mentioning

confidence: 92%

See 2 more Smart Citations

Thyroid hormone and estrogen interact to regulate behavior.

Dellovade

Zhu

Krey

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Environmental perturbations that increase plasma thyroid hormone (T3) concentrations also profoundly affect female reproductive behavior and physiology. We explored whether these effects were mediated by interactions between T3 receptor (TR) and estrogen receptor (ER (6)(7)(8)(9). Decreased ambient temperature can delay puberty in juveniles, and inhibit ovulation, decrease sexual behavior, alter gonadotropin release, and decrease litter size in adults (10-12). Thus, changes in serum thyroid hormone concentrations might be important for signaling environmental conditions to neuroendocrine mechanisms, conceivably by interactions of TRs with ERs on hormone response elements of relevant genes (13). The neural circuitry involved in lordosis, a female rat sexual behavior, is relatively well-understood and is known to be mediated by estrogen acting via hypothalamic ERs (14). However, interactions between thyroid hormones and estrogen on reproductive behavior have not been studied. MATERIALS AND METHODSAnimals and Experimental Design. In the first experiment, we asked whether altered plasma thyroid hormone levels would affect EB-induced lordosis behavior. We used both OVX and thyroidectomized/ovariectomized (TX/OVX) Sprague Dawley rats for this study. We gave daily low doses of EB (2 ,ug, s.c.) to slowly increased plasma estradiol (E2) concentrations in OVX rats, thereby gradually increasing sexual receptivity. T3 was given in high doses (500 ,ug per kg body weight, i.p.) to OVX females to induce hyperthyroid plasma concentrations. In contrast, lower doses of T3 (20 ,ug per kg body weight, i.p) were given to TX/OVX females to increase plasma concentrations within the physiological range.Two weeks after surgery, OVX rats (175-225 g) were treated daily for 10 days with vehicle (n = 13), EB (2 ,g, s.c.; n = 13), high doses of T3 (500 ,ug per kg body weight, i.p.; n = 13), or EB + high doses of T3 (n = 15). TX/OVX females were treated daily for 10 days with vehicle (n = 14), EB (2 ,g; n = 16), low doses of T3 (20 jig per kg body weight i.p.; n = 13), or low doses of T3 + EB (n = 15). T3 was administered every 12 hr at 9:00 a.m. and 9:00 p.m., and EB was injected once a day at 9:00 a.m. In both experiments, animal surgeries were performed by the supplier (Charles River Breeding Laboratories) and animals were maintained on a 12-hr light/12-hr dark cycle (lights on at 7:00 p.m.). Food and water were

show abstract

Section: Resultssupporting

confidence: 70%

mentioning

confidence: 69%

See 1 more Smart Citation

Thyroid hormone and estrogen interact to regulate behavior.

Dellovade

Zhu

Krey

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…In the brain, coadministration of T3 inhibited the induction of oxytocin mRNA in specific subregions of the paraventricular nucleus by EB [23]. Similarly, in a number of other systems, altered concentrations of thyroid hormones and estrogen can mutually affect each other [12, 29, 30]. That such interactions might be important for sexual behavior has been demonstrated in mammals and birds.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, the concept that (through competitive DNA binding, at least) thyroid hormones can interfere with ER action on an ERE-like sequence has been supported by studies in several systems [8, 10, 11, 12]. These molecular interactions might have broad biological consequences.…”

Section: Introductionmentioning

confidence: 91%

Thyroid Hormone Coadministration Inhibits the Estrogen-Stimulated Elevation of Preproenkephalin mRNA in Female Rat Hypothalamic Neurons

Dellovade¹,

Kia²,

Pfaff³

1999

Neuroendocrinology

View full text Add to dashboard Cite

Expression of the enkephalin gene in ventromedial hypothalamus (VMH) of the female rat has been correlated with the performance of lordosis behavior. By antisense DNA evidence, it has been drawn into a causal role as well. Here, we explored whether, parallel to earlier molecular and behavioral results, thyroid hormone coadministration could disrupt the estrogenic induction of preproenkephalin (PPE) mRNA. As expected, estradiol benzoate treatment to ovariectomized rats led to a large and significant increase in PPE gene expression in the VMH. This increase was inhibited by coadministration of thyroid hormone. The thyroid hormone interference in PPE gene expression was specific to the VMH, as there were no significant effects in the central nucleus of the amygdala or in the caudate/putamen. These in situ hybridization histochemical results form a direct parallel both to previous transcriptional measurements and to reproductive behavior assays in which thyroid hormones were able to oppose estrogenic facilitation. Previous evidence supports the notion of competitive DNA binding and protein/protein interactions providing mechanisms for nuclear thyroid hormone receptors to affect estrogen receptor function, but other, additional mechanisms cannot be ruled out. To date, both oxytocin and PPE gene expression represent potential hypothalamic systems by which thyroid hormones could interfere with estrogen-stimulated female rat reproductive behavior.

show abstract

Phylogeny of Estrogen Synthesis, Extragenital Distribution of Estrogen Receptors and Their Developmental Role

Sobek¹,

Patchev²

1999

Estrogens and Antiestrogens I

View full text Add to dashboard Cite

Interference between estradiol and L-triiodothyronine in the control of the proliferation of a pituitary tumor cell line

Cited by 8 publications

References 13 publications

Thyroid hormone and estrogen interact to regulate behavior.

Thyroid hormone and estrogen interact to regulate behavior.

Thyroid Hormone Coadministration Inhibits the Estrogen-Stimulated Elevation of Preproenkephalin mRNA in Female Rat Hypothalamic Neurons

Phylogeny of Estrogen Synthesis, Extragenital Distribution of Estrogen Receptors and Their Developmental Role

Contact Info

Product

Resources

About