Interfacing Living Cells and Spherically Supported Bilayer Lipid Membranes

Madwar, Carolin; Gopalakrishnan, Gopakumar; Lennox, R. Bruce

doi:10.1021/acs.langmuir.5b00862

Cited by 11 publications

(19 citation statements)

References 63 publications

(126 reference statements)

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“…ref. 30 ), this observation suggests that our system has likely reached equilibrium already a few minutes after cooling. This fast equilibration has the further benefit of reducing the effect of substrate-induced drag, which, as shown in ref.…”

Section: Resultsmentioning

confidence: 67%

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Geometric pinning and antimixing in scaffolded lipid vesicles

et al. 2020

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Previous studies on the phase behaviour of multicomponent lipid bilayers found an intricate interplay between membrane geometry and its composition, but a fundamental understanding of curvature-induced effects remains elusive. Thanks to a combination of experiments on lipid vesicles supported by colloidal scaffolds and theoretical work, we demonstrate that the local geometry and global chemical composition of the bilayer determine both the spatial arrangement and the amount of mixing of the lipids. In the mixed phase, a strong geometrical anisotropy can give rise to an antimixed state, where the lipids are mixed, but their relative concentration varies across the membrane. After phase separation, the bilayer organizes in multiple lipid domains, whose location is pinned in specific regions, depending on the substrate curvature and the bending rigidity of the lipid domains. Our results provide critical insights into the phase separation of cellular membranes and, more generally, twodimensional fluids on curved substrates.

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Section: Resultsmentioning

confidence: 67%

“…In control experiments with SLVs prepared from SUVs that were allowed to cool to room temperature before coating, we did not find two or three domains only, but multiple, randomly localized domains similar to results reported in ref. 30 ( Supplementary Fig. 11).…”

Section: Resultsmentioning

confidence: 99%

Geometric pinning and antimixing in scaffolded lipid vesicles

et al. 2020

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“…22, 46 This was probed in a recent spherically-supported biomembrane study using fusion with different sizes of starting lipid vesicles that resulted in differences in organization of lipids and shapes of co-existing domains. 39 Taken together, SUVs here are considerably heterogeneous and a difference in the rate of vesicle fusion further deters homogenization, leading to PLBs with different compositions within the L d /L o co-existence region and fractions of L d /L o content.…”

Section: Resultsmentioning

confidence: 90%

Phase Composition Control in Microsphere-Supported Biomembrane Systems

Fried¹,

Gilchrist³

2017

Langmuir

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The popularization of studies in membrane protein lipid phase coexistence has prompted the development of new techniques to construct and study biomimetic systems with cholesterol-rich lipid microdomains. Here, microsphere supported biomembranes with integrated α-helical peptides, referred to as proteolipobeads (PLBs), were used to model peptide/protein partitioning within DOPC/DPPC/cholesterol phase-separated membranes. Due to the appearance of compositional heterogeneity and impurities in the formation of model PLB assemblies, fluorescence-activated cell sorting (FACS) was used to characterize and sort PLB populations on the basis of disordered phase (Ld) content. In addition, spectral imaging was used to assess the partitioning of FITC-labeled α-helical peptide between fluorescently labeled Ld phase and unlabeled ordered phase (Lo) phase lipid microdomains. The apparent peptide partition coefficient, Kp,app, was measured to be 0.89 ± 0.06, indicating a slight preference of the peptide for the Lo phase. A biomimetic motif of Lo phase concentration enhancement of biotinyl-peptide ligand display in proteolipobeads was also observed. Finally, peptide mobility was measured by FRAP separately in each lipid phase, yielding diffusivities of 0.036±0.005 and 0.014±0.003 µm2/s in the Ld and Lo phases, respectively.

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“…The existence of submicron lateral heterogeneities have been, however, confirmed by thermodynamic phase diagrams [ 4 ], nuclear magnetic resonance [ 5 ], confocal microscopy and fluorescence correlation spectroscopy [ 6 ], and photon fluorescence microscopy [ 7 ],but whether their existence supports biological processes or the biological processes induce their formation is not yet fully elucidated [ 8 ]. Recent evidence, however, provided by spherically supported bilayer lipid membranes, strongly suggests that the dynamic organization of the lipid membrane is indeed a function of lipid microdomains [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Artificial Lipid Membranes: Past, Present, and Future

et al. 2017

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The multifaceted role of biological membranes prompted early the development of artificial lipid-based models with a primary view of reconstituting the natural functions in vitro so as to study and exploit chemoreception for sensor engineering. Over the years, a fair amount of knowledge on the artificial lipid membranes, as both, suspended or supported lipid films and liposomes, has been disseminated and has helped to diversify and expand initial scopes. Artificial lipid membranes can be constructed by several methods, stabilized by various means, functionalized in a variety of ways, experimented upon intensively, and broadly utilized in sensor development, drug testing, drug discovery or as molecular tools and research probes for elucidating the mechanics and the mechanisms of biological membranes. This paper reviews the state-of-the-art, discusses the diversity of applications, and presents future perspectives. The newly-introduced field of artificial cells further broadens the applicability of artificial membranes in studying the evolution of life.

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Interfacing Living Cells and Spherically Supported Bilayer Lipid Membranes

Cited by 11 publications

References 63 publications

Geometric pinning and antimixing in scaffolded lipid vesicles

Geometric pinning and antimixing in scaffolded lipid vesicles

Phase Composition Control in Microsphere-Supported Biomembrane Systems

Artificial Lipid Membranes: Past, Present, and Future

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