Interfacing Electrogenic Cells with 3D Nanoelectrodes: Position, Shape, and Size Matter

Santoro, Francesca; Dasgupta, Sabyasachi; Schnitker, Jan; Auth, Thorsten; Neumann, Elmar; Panaitov, G.; Gompper, Gerhard; Offenhäusser, Andreas

doi:10.1021/nn500393p

Cited by 104 publications

(122 citation statements)

References 51 publications

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“…4). The evaluation of the true interface cleft distance in vivo is a question of current debate, as the fixation of cells for electron microscopy can produce a cleft morphology different from what is present in vivo 15, 54 . The cleft distance has been determined to change as much as 10–50 nm following fixation in some cases, even where cryofixation methods are employed.…”

Section: Resultsmentioning

confidence: 99%

“…Like RBLs, HEKs form a high interfacial area contact with hedgehog crystals, (Fig. 5), with occasional examples of engulfment-type processes 15 clearly occurring (Fig. 5b).…”

Section: Resultsmentioning

confidence: 99%

“…In all of these applications, interrogation of intracellular events relies on sharp high-aspect ratio nanostructures 9, 13, 14 . Artificial high-aspect nanostructures have similarly been a focus of interest for electronic interfacing with living cells, being sought after for applications in high-quality extracellular and intracellular electrophysiology 7, 15 recording and stimulation, and for providing a bridge into the cytosol for both delivery and intracellular sensing 10, 11 . Inorganic materials, especially silicon, and metals like platinum and gold predominate in all these applications.…”

Section: Introductionmentioning

confidence: 99%

“…Inorganic materials, especially silicon, and metals like platinum and gold predominate in all these applications. A common goal is getting as close an interface to the cell as possible, forming a minimal cleft, and ideally with large area 13, 15 . Optimising such structures is especially critical in the case of (opto)electronic interfaces, where the cleft between the cell and electronic element results in electric field screening and poor coupling 16–18 .…”

Section: Introductionmentioning

confidence: 99%

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Cellular interfaces with hydrogen-bonded organic semiconductor hierarchical nanocrystals

et al. 2017

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Successful formation of electronic interfaces between living cells and semiconductors hinges on being able to obtain an extremely close and high surface-area contact, which preserves both cell viability and semiconductor performance. To accomplish this, we introduce organic semiconductor assemblies consisting of a hierarchical arrangement of nanocrystals. These are synthesised via a colloidal chemical route that transforms the nontoxic commercial pigment quinacridone into various biomimetic three-dimensional arrangements of nanocrystals. Through a tuning of parameters such as precursor concentration, ligands and additives, we obtain complex size and shape control at room temperature. We elaborate hedgehog-shaped crystals comprising nanoscale needles or daggers that form intimate interfaces with the cell membrane, minimising the cleft with single cells without apparent detriment to viability. Excitation of such interfaces with light leads to effective cellular photostimulation. We find reversible light-induced conductance changes in ion-selective or temperature-gated channels.

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Section: Resultsmentioning

confidence: 99%

“…Like RBLs, HEKs form a high interfacial area contact with hedgehog crystals, (Fig. 5), with occasional examples of engulfment-type processes 15 clearly occurring (Fig. 5b).…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cellular interfaces with hydrogen-bonded organic semiconductor hierarchical nanocrystals

et al. 2017

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“…This can be achieved by using nanostructured electrodes instead of planar electrodes that become engulfed by overlying cells. 1,9 A second approach is to decrease the electrode impedance. To record activity from single cells, the use of a smaller electrode is preferable, but this comes at the cost of increased electrode impedance, which decreases the signal.…”

mentioning

confidence: 99%

An electrically resistive sheet of glial cells for amplifying signals of neuronal extracellular recordings

Matsumura

Yamamoto

Niwano

et al. 2016

Applied Physics Letters

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Electrical signals of neuronal cells can be recorded non-invasively and with a high degree of temporal resolution using multielectrode arrays (MEAs). However, signals that are recorded with these devices are small, usually 0.01%-0.1% of intracellular recordings. Here, we show that the amplitude of neuronal signals recorded with MEA devices can be amplified by covering neuronal networks with an electrically resistive sheet. The resistive sheet used in this study is a monolayer of glial cells, supportive cells in the brain. The glial cells were grown on a collagen-gel film that is permeable to oxygen and other nutrients. The impedance of the glial sheet was measured by electrochemical impedance spectroscopy, and equivalent circuit simulations were performed to theoretically investigate the effect of covering the neurons with such a resistive sheet. Finally, the effect of the resistive glial sheet was confirmed experimentally, showing a 6-fold increase in neuronal signals. This technique feasibly amplifies signals of MEA recordings. V C 2016 AIP Publishing LLC. Microelectrode array (MEA) technology is widely used to record trains of action potentials from cultured neurons, cardiomyocytes, or brain slice preparations. [1][2][3][4] The major advantage of this method lies in its high temporal resolution (>10 kHz) and non-invasiveness. MEA recordings have been used both in fundamental studies, e.g., to analyse activity patterns of cultured neuronal networks, 5,6 and in pharmacological research, for screening lead compounds in vitro. 7,8 However, small signals that can be detected through extracellularly positioned microelectrodes inhibit MEAs from being used in further applications such as studies of subthreshold activity. The amplitude of extracellularly recorded signals is usually in the order of ten to a hundred microvolts, which is 3-4 orders of magnitude lower than the intracellular change of membrane potential in an action potential ($100 mV).Various attempts have been made to overcome this issue. One approach involves increasing the seal resistance between the cell and the electrode. This can be achieved by using nanostructured electrodes instead of planar electrodes that become engulfed by overlying cells. 1,9 A second approach is to decrease the electrode impedance. To record activity from single cells, the use of a smaller electrode is preferable, but this comes at the cost of increased electrode impedance, which decreases the signal. Wolfrum et al. overcame this dilemma by creating micropores that interface the cells to larger electrodes. 10,11 A third approach involves decreasing the membrane impedance at the cell-electrode junction. In practice, this involves transiently rapturing the cell membrane by applying a zap voltage to a nanostructured electrode, thus allowing the electrodes to enter the cell and perform pseudo-intracellular recording until the raptured membrane reorganizes. 1,12,13 In this letter, we propose an alternative approach to increase the neuron-electrode seal for amplifying signals in ex...

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Vertically Aligned Carbon Nanotubes as Platform for Biomimetically Inspired Mechanical Sensing, Bioactive Surfaces, and Electrical Cell Interfacing

Schneider

2017

Adv. Biosys.

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Vertically aligned carbon nanotubes (VACNTs) are one dimensional carbon objects anchored atop of a solid substrate. They are geometrically fixed in contrast to their counterparts, randomly oriented carbon nanotubes (CNTs). In this progress report, the breadth in which these one dimensional, mechanically flexible, though robust and electrical conducting carbon nanostructures can be employed as functional material is shown and our research is put in perspective to work in the last five to ten years. The connection between the different areas touched in this report is the biomimetic‐materials approach, which rely on the hairy morphology of VACNTs. These properties in connection with their electrical conductivity offer possibilities towards new functional features and applications of VACNTs. To appreciate the possibilities of biomimetic research with VACNTs, first their material characteristics are given to make the reader familiar with specific features of their synthesis, the peculiarities in arranging and controlling the morphology of CNTs in a vertical alignment as well as a current understanding of these properties on a microscopic basis. In doing so, similarities as well as differences, which offer new possibilities for biomimetic studies of VACNTS with respect to multiwalled randomly oriented CNTs, will become clear.

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Interfacing Electrogenic Cells with 3D Nanoelectrodes: Position, Shape, and Size Matter

Cited by 104 publications

References 51 publications

Cellular interfaces with hydrogen-bonded organic semiconductor hierarchical nanocrystals

Cellular interfaces with hydrogen-bonded organic semiconductor hierarchical nanocrystals

An electrically resistive sheet of glial cells for amplifying signals of neuronal extracellular recordings

Vertically Aligned Carbon Nanotubes as Platform for Biomimetically Inspired Mechanical Sensing, Bioactive Surfaces, and Electrical Cell Interfacing

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