Interfacial Engineering by Proteins: Exfoliation and Functionalization of Graphene by Hydrophobins

Laaksonen, Päivi; Kainlauri, Markku; Laaksonen, Timo; Shchepetov, A.; Jiang, Hua; Ahopelto, Jouni; Linder, Markus B.

doi:10.1002/anie.201001806

Cited by 162 publications

(122 citation statements)

References 28 publications

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“…Actually, the strong hydrophobic interaction between the graphene sheets and protein molecules can be applied in graphene preparation. As shown in Figure 4 , by using the strong hydrophobic interaction between hydrophobic protein hydrophobins (HFBI) and graphene, the graphite could be exfoliated, and the graphene sheets functionalized with HFBI were generated simultaneously [117] . In water, a monolayer of amphiphilic HFBI is spontaneously adsorbed on the hydrophobic surface of graphite.…”

Section: Noncovalent Adsorption Of the Protein/ Enzyme Molecules On Tmentioning

confidence: 99%

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Interactions of graphene and graphene oxide with proteins and peptides

Zhang

Guo³

et al. 2013

Nanotechnology Reviews

198

105

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This review selectively describes the recent progress in the interactions of proteins (enzymes) and shortchain peptides with graphene and graphene oxide (GO). Particularly, the advances of the immobilization mechanisms of enzymes on graphene and GO, the catalytic properties of the immobilized enzymes, and their applications are summarized in detail. The interfacings of the peptides with graphene and GO, the as assembled conjugates, and their potential applications are discussed briefly. The possible ongoing development for the assembly of conjugates of graphene and GO with proteins and peptides in a controlled manner is speculated upon.

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Section: Noncovalent Adsorption Of the Protein/ Enzyme Molecules On Tmentioning

confidence: 99%

“…The N terminus, to which sequences were added in the engineered variants, is indicated by an arrow. (B) HFBI-facilitated exfoliation of graphene [117] .…”

Section: Noncovalent Adsorption Of the Protein/ Enzyme Molecules On Tmentioning

confidence: 99%

Interactions of graphene and graphene oxide with proteins and peptides

Zhang

Guo³

et al. 2013

Nanotechnology Reviews

198

105

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“…Typically class I hydrophobins exhibit irreversible assembly including conformational changes at interface [16], whereas class II hydrophobins are structurally more stable and such conformational changes have not been observed. Due to their globular and stable conformation and small size, class II hydrophobins have been employed in formation of welldefined layers at various interfaces [17][18][19]. By genetic engineering, additional functionalities can be brought to the protein layers by fusing the anchoring group with a second functional domain allowing functionalization of such interfacial layer [20,21].…”

Section: Introductionmentioning

confidence: 99%

Engineered Hydrophobin for Biomimetic Mineralization of Functional Calcium Carbonate Microparticles

Heinonen¹,

Laaksonen²,

Hentze³

2014

JBNB

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In this study, the modified hydrophobin, engineered for biomimetic mineralization, has been employed as a structure-directing agent for mineralization of calcium carbonate. For the first time amphiphilic calcium carbonate particles have been obtained, using engineered proteins. The mineral microparticles have been characterized by optical microscopy, scanning electron microscopy (SEM) and X-ray diffraction (XRD). While mineralization in the presence of non-modified hydrophobin results in polymorph mineral structures, uniform microspheres with an average particle diameter of one micron are obtained by employing hydrophobin which has been modified with an additional ceramophilic protein sequence. Owing to the tri-functionality of the modified hydrophobin (hydrophilic, hydrophobic and ceramophilic), the obtained mineral microparticles exhibit amphiphilic properties. Potential applications are in the areas of functional fillers and pigments, like biomedical and composite materials. Pickering emulsions have been prepared as a demonstration of the emulsion-stabilizing properties of the obtained amphiphilic mineral microspheres. The structure-directing effects of the studied engineered hydrophobins are compared with those of synthetic polymers (i.e. polycarboxylates) used as crystallization and scaling inhibitors in industrial applications.

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“…A report exists of genetically engineered proteins capable of exfoliating GR, and it is also possible that other proteins having a structure and energy matching with GR may be able to do the same. 8 We report results that show CL-GR was surprisingly able to increase thermal conductivity at a very low concentration. Thus, through the use of CL, this new material and process may improve the use of graphene in numerous medical applications.…”

Section: Introductionmentioning

confidence: 84%

Colloidal graphite/graphene nanostructures using collagen showing enhanced thermal conductivity

Bhattacharya¹,

Dhar²,

Das³

et al. 2014

IJN

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In the present study, the exfoliation of natural graphite (GR) directly to colloidal GR/graphene (G) nanostructures using collagen (CL) was studied as a safe and scalable process, akin to numerous natural processes and hence can be termed “biomimetic”. Although the exfoliation and functionalization takes place in just 1 day, it takes about 7 days for the nano GR/G flakes to stabilize. The predominantly aromatic residues of the triple helical CL forms its own special micro and nanoarchitecture in acetic acid dispersions. This, with the help of hydrophobic and electrostatic forces, interacts with GR and breaks it down to nanostructures, forming a stable colloidal dispersion. Surface enhanced Raman spectroscopy, X-ray diffraction, photoluminescence, fluorescence, and X-ray photoelectron spectroscopy of the colloid show the interaction between GR and CL on day 1 and 7. Differential interference contrast images in the liquid state clearly reveal how the GR flakes are entrapped in the CL fibrils, with a corresponding fluorescence image showing the intercalation of CL within GR. Atomic force microscopy of graphene-collagen coated on glass substrates shows an average flake size of 350 nm, and the hexagonal diffraction pattern and thickness contours of the G flakes from transmission electron microscopy confirm ≤ five layers of G. Thermal conductivity of the colloid shows an approximate 17% enhancement for a volume fraction of less than approximately 0.00005 of G. Thus, through the use of CL, this new material and process may improve the use of G in terms of biocompatibility for numerous medical applications that currently employ G, such as internally controlled drug-delivery assisted thermal ablation of carcinoma cells.

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Interfacial Engineering by Proteins: Exfoliation and Functionalization of Graphene by Hydrophobins

Cited by 162 publications

References 28 publications

Interactions of graphene and graphene oxide with proteins and peptides

Interactions of graphene and graphene oxide with proteins and peptides

Engineered Hydrophobin for Biomimetic Mineralization of Functional Calcium Carbonate Microparticles

Colloidal graphite/graphene nanostructures using collagen showing enhanced thermal conductivity

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