Interfacial biodegradation of phenanthrene in bacteria-carboxymethyl cellulose-stabilized Pickering emulsions

Pan, Tao; Liu, Congyang; Wang, Meini; Zhang, Jiameng

doi:10.1007/s00253-022-11952-9

Cited by 6 publications

(5 citation statements)

References 28 publications

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“…Despite the absence of interfacial cells, PHE biodegradation remained rapid in TET (2d) or DEHP (5d). This suggests a surfactant-mediated uptake model, where cells utilize surfactant micelles to access and degrade PHE within the emulsions. , …”

Section: Resultsmentioning

confidence: 99%

“…The WY10 was a highly effective PAH-degrading bacterium that can mineralize PHE and pyrene . TET, a primary component of oil and a common substitute for petroleum in polluted soil and water, contrasts with DEHP, a soil-dwelling plasticizer frequently studied for its influence on PAH bioavailability. , By employing PHE, a well-established PAH model pollutant, , and leveraging WY10′s reported PHE-degrading prowess and emulsion-stabilizing hydrophobic surface, this work delves into the complex mechanisms governing bacterial–surfactant interactions and their implications for PAH biodegradation across diverse environmental settings.…”

Section: Introductionmentioning

confidence: 99%

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From Antagonism to Enhancement: Triton X-100 Surfactant Affects Phenanthrene Interfacial Biodegradation by Mycobacteria through a Shift in Uptake Mechanisms

Wang,

Zhang,

et al. 2024

Langmuir

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Polycyclic aromatic hydrocarbons (PAHs), as persistent environmental pollutants, often reside in nonaqueous-phase liquids (NAPLs). Mycobacterium sp. WY10, boasting highly hydrophobic surfaces, can adsorb to the oil–water interface, stabilizing the Pickering emulsion and directly accessing PAHs for biodegradation. We investigated the impact of Triton X-100 (TX100) on this interfacial uptake of phenanthrene (PHE) by Mycobacteria, using n-tetradecane (TET) and bis-(2-ethylhexyl) phthalate (DEHP) as NAPLs. Interfacial tension, phase behavior, and emulsion stability studies, alongside confocal laser scanning microscopy and electron microscope observations, unveiled the intricate interplay. In surfactant-free systems, Mycobacteria formed stable W/O Pickering emulsions, directly degrading PHE within the NAPLs because of their intimate contact. Introducing low-dose TX100 disrupted this relationship. Preferentially binding to the cells, the surfactant drastically increased the cell hydrophobicity, triggering desorption from the interface and phase separation. Consequently, PAH degradation plummeted due to hindered NAPL access. Higher TX100 concentrations flipped the script, creating surfactant-stabilized O/W emulsions devoid of interfacial cells. Surprisingly, PAH degradation remained efficient. This paradox can be attributed to NAPL emulsification, driven by the surfactant, which enhanced mass transfer and brought the substrate closer to the cells, despite their absence at the interface. This study sheds light on the complex effect of surfactants on Mycobacteria and PAH uptake, revealing an antagonistic effect at low concentrations that ultimately leads to enhanced degradation through emulsification at higher doses. These findings offer valuable insights into optimizing bioremediation strategies in PAH-contaminated environments.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

From Antagonism to Enhancement: Triton X-100 Surfactant Affects Phenanthrene Interfacial Biodegradation by Mycobacteria through a Shift in Uptake Mechanisms

Wang,

Zhang,

et al. 2024

Langmuir

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“…Both strains were screened from soils with high RE background values and long-term petrochemical pollution. Previous studies have revealed that CFP312 has good adaptability to naphthalene and phenanthrene, thus strengthening degradation systems (Liu et al 2021;Pan et al 2022). In this work, we are interested in determining whether the RE toxicity resistance and PAHs biodegradation of two strains from the same niche have a synergistic effect.…”

Section: Introductionmentioning

confidence: 99%

“…2021; Pan et al . 2022). In this work, we are interested in determining whether the RE toxicity resistance and PAHs biodegradation of two strains from the same niche have a synergistic effect.…”

Section: Introductionmentioning

confidence: 99%

Synergistic effects of a functional bacterial consortium on enhancing phenanthrene biodegradation and counteracting rare earth biotoxicity in liquid and slurry systems

Wang

Liu

Zhang

et al. 2022

Letters in Applied Microbiology

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Significance and Impact of the Study: This study focused on the effect of rare earth (REs) on the biodegradation of polycyclic aromatic hydrocarbons (PAHs). The RE ions Ce 3+ and Y 3+ inhibited Moraxella osloensis CFP312 from degrading phenanthrene without affecting its glucose utilization. This inhibition effect can be relieved through co-cultivation with Bacillus subtilis MSP117, which has high adsorption capacity for RE ions in liquid and slurry systems. MSP117 adsorbed and fixed RE ions on its cell surfaces, thereby reducing the bioavailability of RE ions. This study provides a feasible way for the bioremediation of the co-pollution of RE and organic pollutants.

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“…In fact, an interaction between bacterial cells and chitosan even solid phytosterol crystals has been observed during the microbial transformation of phytosterols, which leads to demulsification of the Pickering emulsions stabilized by hydrophobic Mycobacteria cells and reduces the biocatalytic performance of bacterial cells, such as degradation of phytosterols, microbial growth (biomass), even release of CO 2 . Addition of chitosan, as well as carboxymethyl cellulose, to enhance the biodegradation of alkanes as well as microbial growth is also reported. Till now, there is no information about the influence of nonionic surfactants on the biocatalytic performance of natural alkane-degrading bacteria from the viewpoint of interfacial biocatalysis.…”

Section: Introductionmentioning

confidence: 99%

Surfactants Influencing the Biocatalytic Performance of Natural Alkane-Degrading Bacteria via Interfacial Biocatalysis in Pickering Emulsions

Ali,

Zhang,

Wang

2023

Langmuir

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Setting superhydrophobic Mycobacterium sp. as an example, the hydrophobic bacteria acting as demulsifying agents of surfactant-stabilized conventional emulsions, vice versa, the synergistic/antagonistic influence of nonionic surfactants (Tween 80 or Span 80) on the stability of the bacteria-stabilized Pickering emulsions was investigated. At the same time, the activated/ suppression effect of nonionic surfactants on microbial degradation of tetradecane, which exhibited a dose−response relationship, was also found. The hydrophobic bacteria acting as demulsifying agents and the suppression influence of nonionic surfactants on the biocatalytic performance (indexing as biomass) of natural alkane-degrading bacteria, believed to be totally separated concepts previously, are for the first time found to be closely related to in situ surface modification of bacteria with nonionic surfactants. During the degradation of tetradecane by Mycobacterium sp. in the presence of nonionic surfactants, demulsification due to the bacteria acting as demulsifying agents and interfacial biocatalysis in the bacteria-stabilized Pickering emulsions are involved, which provides useful information to select optimal dispersants for marine oil spills.

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Interfacial biodegradation of phenanthrene in bacteria-carboxymethyl cellulose-stabilized Pickering emulsions

Cited by 6 publications

References 28 publications

From Antagonism to Enhancement: Triton X-100 Surfactant Affects Phenanthrene Interfacial Biodegradation by Mycobacteria through a Shift in Uptake Mechanisms

From Antagonism to Enhancement: Triton X-100 Surfactant Affects Phenanthrene Interfacial Biodegradation by Mycobacteria through a Shift in Uptake Mechanisms

Synergistic effects of a functional bacterial consortium on enhancing phenanthrene biodegradation and counteracting rare earth biotoxicity in liquid and slurry systems

Surfactants Influencing the Biocatalytic Performance of Natural Alkane-Degrading Bacteria via Interfacial Biocatalysis in Pickering Emulsions

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