Interfacial Behavior of Fumonisin B1 Toxin and Its Degradation on the Membrane

Sharma, Shiv K.; Sharma, Sijan Poudel; Miller, Darlene; Parel, Jean Marie; Leblanc, Roger M.

doi:10.1021/acs.langmuir.8b03505

Cited by 7 publications

(8 citation statements)

References 28 publications

(45 reference statements)

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“…We were also interested to know the surface chemistry of the conjugate as its surface properties determine its adsorption behavior on the surface of an electrode [30]. Surface chemistry and spectroscopic studies can also be used to reveal the interaction of the analytes at the air/subphase interface and in bulk solution.…”

Section: Resultsmentioning

confidence: 99%

Conjugation of Carbon Dots with β-Galactosidase Enzyme: Surface Chemistry and Use in Biosensing

Sharma

Mićić

et al. 2019

Molecules

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Nanoparticles have been conjugated to biological systems for numerous applications such as self-assembly, sensing, imaging, and therapy. Development of more reliable and robust biosensors that exhibit high response rate, increased detection limit, and enhanced useful lifetime is in high demand. We have developed a sensing platform by the conjugation of β-galactosidase, a crucial enzyme, with lab-synthesized gel-like carbon dots (CDs) which have high luminescence, photostability, and easy surface functionalization. We found that the conjugated enzyme exhibited higher stability towards temperature and pH changes in comparison to the native enzyme. This enriched property of the enzyme was distinctly used to develop a stable, reliable, robust biosensor. The detection limit of the biosensor was found to be 2.9 × 10−4 M, whereas its sensitivity was 0.81 µA·mmol−1·cm−2. Further, we used the Langmuir monolayer technique to understand the surface properties of the conjugated enzyme. It was found that the conjugate was highly stable at the air/subphase interface which additionally reinforces the suitability of the use of the conjugated enzyme for the biosensing applications.

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Section: Resultsmentioning

confidence: 99%

Conjugation of Carbon Dots with β-Galactosidase Enzyme: Surface Chemistry and Use in Biosensing

Sharma

Mićić

et al. 2019

Molecules

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“…The Langmuir monolayer is an important constituent of surface chemistry. Its use first discerned lipid bilayers of the cell membrane . Subsequently, the Langmuir monolayer was widely used to measure molecular dimensions .…”

Section: Carbon Dots In Surface Chemistry: Langmuir Monolayer Of Carb...mentioning

confidence: 99%

“…Its use first discerned lipid bilayers of the cell membrane . Subsequently, the Langmuir monolayer was widely used to measure molecular dimensions . Most importantly, it can mimic the cell membrane for studies on the impacts of diverse proteins, pharmaceuticals, and toxins. , Nonetheless, in order to form a Langmuir monolayer, molecules have to be insoluble in water, which largely limits its applicability with a number of hydrophilic molecules and NPs including most CDs.…”

Section: Carbon Dots In Surface Chemistry: Langmuir Monolayer Of Carb...mentioning

confidence: 99%

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Structure–Property–Activity Relationships in Carbon Dots

2022

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Carbon dots (CDs) are one of the most versatile nanomaterials discovered in the 21st century. They possess many properties and thus hold potentials in diverse applications. While an increasing amount of attention has been given to these novel nanoparticles, the broad scientific community is actively engaged in exploring their limits. Recent studies on the fractionalization and assembly of CDs further push the limits beyond just CDs and demonstrate that CDs are both a mixture of heterogeneous fractions and promising building blocks for assembly of large carbon-based materials. With CDs moving forward toward both microscopic and macroscopic levels, a good understanding of the structure−property−activity relationships is essential to forecasting the future of CDs. Hence, in this Perspective, structure−property− activity relationships are highlighted based on the repeatedly verified findings in CDs. In addition, studies on CD fractionalization and assembly are briefly summarized in this Perspective. Eventually, these structure−property−activity relationships and controllability are essential for the development of CDs with desired properties for various applications especially in photochemistry, electrochemistry, nanomedicine, and surface chemistry. In summary, in our opinion, since 2004 until the present, history has witnessed a great development of CDs although there is still some room for more studies. Also, considering many attractive properties, structure−property−activity relationships, and the building block nature of CDs, a variety of carbon-based materials of interest can be constructed from CDs with control. They can help reduce blind trials in the development of carbon-based materials, which is of great significance in materials science, chemistry, and any fields related to the applications.

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“…Exposure to fumonisin could also induce epigenetic changes such as DNA methylation and hypomethylation in rat glioma cells and human intestinal and hepatoma cells [19]. Apart from neurotoxicity, hepatotoxicity, nephrotoxicity, and carcinogenicity, FB1 has also been studied in corneal infections, due to its ability to form Langmuir monolayers on liquid surfaces [21]. Besides, some geographical studies have correlated the prevalence of esophageal cancer in humans with the presence of FB1 and FB2 in regional crops [22,23].…”

Section: Effects Of Fumonisin B1 On Healthmentioning

confidence: 99%

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Mirón-Mérida

Gong²,

Goycoolea³

2020

Preprint

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Mycotoxin contamination is a current issue affecting several crops and processed products worldwide. Among the diverse mycotoxin group, fumonisin B1 (FB1) has become a relevant compound because of its adverse effects in the food chain. Conventional analytical methods previously proposed to quantify FB1 comprise LC-MS, HPLC-FLD and ELISA, while novel approaches integrate different sensing platforms and fluorescently labelled agents in combination with antibodies. Nevertheless, such methods could be expensive, time-consuming and require experience. Aptamers (ssDNA) are promising alternatives to overcome some of the drawbacks of conventional analytical methods, their high affinity through specific aptamer-target binding has been exploited in various designs attaining favorable limits of detection (LOD). So far, two aptamers specific to FB1 have been reported, and their modified and shortened sequences have been explored for a successful target quantification. In this critical review spanning the last eight years, we have conducted a systematic comparison based on principal component analysis of the aptamer-based techniques for FB1, compared with chromatographic, immunological and other analytical methods. We have also conducted an <i>in-silico</i> prediction of the folded structure of both aptamers under their reported conditions. The potential of aptasensors for the future development of highly sensitive FB1 testing methods is emphasized.

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Interfacial Behavior of Fumonisin B1 Toxin and Its Degradation on the Membrane

Cited by 7 publications

References 28 publications

Conjugation of Carbon Dots with β-Galactosidase Enzyme: Surface Chemistry and Use in Biosensing

Conjugation of Carbon Dots with β-Galactosidase Enzyme: Surface Chemistry and Use in Biosensing

Structure–Property–Activity Relationships in Carbon Dots

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

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