Interfacial and structural characteristics of polyelectrolyte multilayers used as cushions for supported lipid bilayers

Abstract

The surface properties of polyelectrolyte multilayers (PEMs) obtained via sequential adsorption of oppositely charged polyions from their solutions and used as cushions for supported lipid bilayers were investigated. Five types of polyelectrolytes were used: cationic polyethyleneimine (PEI), poly(diallyldimethylammonium)chloride (PDADMAC), and poly-l-lysine hydrobromide (PLL); and anionic polysodium 4-styrenesulfonate (PSS) and poly-l-glutamic acid sodium (PGA). The wettability and surface free energy of the PEMs were determined by contact angle measurements using sessile drop analysis. Electrokinetic characterisation of the studied films was performed by streaming potential measurements of selected multilayers and the structure of the polyelectrolyte multilayer was characterized by synchrotron X-ray reflectometry. The examined physicochemical properties of the PEMs were correlated with the kinetics of the formation of supported lipid bilayers atop the PEM cushion.

Supported lipid bilayers with encapsulated quantum dots (QDs) via liposome fusion: effect of QD size on bilayer formation and structure

“…Moreover, hydrogen bonding formation among PE lipids and between the PE headgroup and the solid support could promote vesicle fusion and SLB formation. 44,45 The PEI film has been previously shown [46][47][48][49][50] as an effective anchor for subsequent adsorption of nanoobjects such as polyelectrolytes, nanoparticles, or liposomes. In our study, the PEI monolayer preparation used the same procedure as that in these studies, and the detailed characterization of such PEI monolayers has also been reported by Adamczyk et al.…”

Modulation of the internal structure and surface properties of natural and synthetic polymer matrices by graphene oxide doping

“…In contrast, roughness parameters of the (wPE) 9 GO film are higher than that of the wPE film. This difference can be attributed to the distinct growth mechanism of multilayers composed of interpenetrating vs non‐penetrating PE which has been shown to affect the surface properties of PEM films …”

Bénard–Marangoni Dendrites upon Evaporation of a Reactive ZnO Nanofluid Droplet: Effect of Substrate Chemistry

“…As the evaporation took place, the pinned droplet would thin, and the apparent CA at the pinned periphery would decrease to the values of respective nano/microfluids, before the contact line receded upon further evaporation. (38)(39)(40). Resolving this small controversy is beyond the scope of this study.…”