Interface potential sensing from adsorption of human serum albumin (HSA) on carbon nanotube (CNT) monitored by zero current potentiometry for HSA determination

Wang, Huan; Wu, Yi; Song, Junfeng

doi:10.1016/j.bios.2015.05.013

Cited by 16 publications

(5 citation statements)

References 34 publications

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“…The authors loaded 0.5 wt% of bovine serum albumin (BSA) as a blocking agent before introducing HSA because non-specific binding can be a significant issue affecting the performance of this type of sensor. As a result, they successfully measured HSA with a LOD of 18.6 μg/L by measuring the electrical conductance of SWCNT-FET, which showed improved sensitivity compared to those of previously developed HSA sensors based on different methods such as total internal reflected resonance light scattering [ 90 ], surface-enhanced Raman scattering [ 91 ], and zero-current potentiometry [ 92 ]. Moreover, the biosensor achieved a real-time response with increasing HSA concentrations from 100 pM to 10 μM in a healthy human urine sample ( Figure 4 b).…”

Section: Detecting Analytes In Urine For Urinalysismentioning

confidence: 99%

Recent Advances in Biosensor Technologies for Point-of-Care Urinalysis

et al. 2022

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Human urine samples are non-invasive, readily available, and contain several components that can provide useful indicators of the health status of patients. Hence, urine is a desirable and important template to aid in the diagnosis of common clinical conditions. Conventional methods such as dipstick tests, urine culture, and urine microscopy are commonly used for urinalysis. Among them, the dipstick test is undoubtedly the most popular owing to its ease of use, low cost, and quick response. Despite these advantages, the dipstick test has limitations in terms of sensitivity, selectivity, reusability, and quantitative evaluation of diseases. Various biosensor technologies give it the potential for being developed into point-of-care (POC) applications by overcoming these limitations of the dipstick test. Here, we present a review of the biosensor technologies available to identify urine-based biomarkers that are typically detected by the dipstick test and discuss the present limitations and challenges that future development for their translation into POC applications for urinalysis.

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Section: Detecting Analytes In Urine For Urinalysismentioning

confidence: 99%

Recent Advances in Biosensor Technologies for Point-of-Care Urinalysis

et al. 2022

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“…As an example, Wang et al used a potentiometric approach to measure the human serum albumin (HSA) adsorption on MWCNT. When the HSA was adsorbed on the surface of the MWCNT an electrical potential was produced which was linearly related to the zero current potential ranging from 2.8 × 10 −8 to 3.4 × 10 −7 M with a detection limit of 2 × 10 −8 M (Wang et al 2015a). In another study, potentiometric sensing using a hybrid structure of transduction capabilities of SWCNTs and a protein-specific RNA Aptamer were used to detect protein at concentrations as low as 4 fM for real-time detection (Zelada-Guillén et al 2013).…”

Section: Lab-on-a-chipmentioning

confidence: 99%

Carbon nanotubes in microfluidic lab-on-a-chip technology: current trends and future perspectives

Ghasemi

Amiri

Zare

et al. 2017

Microfluid Nanofluid

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Advanced nanomaterials such as carbon nano-tubes (CNTs) display unprecedented properties such as strength, electrical conductance, thermal stability, and intriguing optical properties. These properties of CNT allow construction of small microfluidic devices leading to miniaturization of analyses previously conducted on a laboratory bench. With dimensions of only millimeters to a few square centimeters, these devices are called lab-on-a-chip (LOC). A LOC device requires a multidisciplinary contribution from different fields and offers automation, portability, and high-throughput screening along with a significant reduction in reagent consumption. Today, CNT can play a vital role in many parts of a LOC such as membrane channels, sensors and channel walls. This review paper provides an overview of recent trends in the use of CNT in LOC devices and covers challenges and recent advances in the field. CNTs are also reviewed in terms of synthesis, integration techniques, functionalization and superhydrophobicity. In addition, the toxicity of these nanomaterials is reviewed as a major challenge and recent approaches addressing this issue are discussed.

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“…OCP measurements with modified electrodes or redox-responsive additives have been commonly used for a range of applications, including sensing, − kinetic studies, , and binding affinity measurements . Scientists have also leveraged potentiometry for interdisciplinary applications including monitoring oxidative polymerization, detecting nanoparticle sizes and collisions, and monitoring oxygen levels in the winemaking process. − …”

Section: Introductionmentioning

confidence: 99%