Interface of Phospholipase Activity, Immune Cell Function, and Atherosclerosis

Schilke, Robert M; Blackburn, Cassidy M.R.; Bamgbose, Temitayo; Woolard, Matthew D.

doi:10.3390/biom10101449

Cited by 14 publications

(5 citation statements)

References 153 publications

(227 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Direct pro-inflammatory and atherogenic effects of LPC have become apparent over the past 30 years. Nowadays, there is increasing evidence that LPC has also anti-inflammatory properties, making its profile more complex than initially assumed ( Schilke et al, 2020 ). These controversial effects of LPC are presumably caused by differences in length and/or the saturation state of the fatty acyl chain ( Tan et al, 2020 ).…”

Section: Lysophospholipid As Inflammation and Disease Markersmentioning

confidence: 99%

Phospholipases and Reactive Oxygen Species Derived Lipid Biomarkers in Healthy and Diseased Humans and Animals – A Focus on Lysophosphatidylcholine

et al. 2021

View full text Add to dashboard Cite

Phospholipids (PL) are converted into lipid biomarkers by the action of phospholipases and reactive oxygen species (ROS), which are activated or released under certain physiological and pathophysiological conditions. Therefore, the in vivo concentration of such lipid biomarkers [e.g., lysophospholipids (LPLs)] is altered in humans and animals under different conditions such as inflammation, stress, medication, and nutrition. LPLs are particularly interesting because they are known to possess pro- and anti-inflammatory properties and may be generated by two different pathways: either by the influence of phospholipase A2 or by different reactive oxygen species that are generated in significant amounts under inflammatory conditions. Both lead to the cleavage of unsaturated acyl residues. This review provides a short summary of the mechanisms by which lipid biomarkers are generated under in vitro and in vivo conditions. The focus will be on lysophosphatidylcholine (LPC) because usually, this is the LPL species which occurs in the highest concentration and is, thus, easily detectable by chromatographic and spectroscopic methods. Finally, the effects of lipid biomarkers as signaling molecules and their roles in different human and animal pathologies such as infertility, cancer, atherosclerosis, and aging will be shortly discussed.

show abstract

Section: Lysophospholipid As Inflammation and Disease Markersmentioning

confidence: 99%

Phospholipases and Reactive Oxygen Species Derived Lipid Biomarkers in Healthy and Diseased Humans and Animals – A Focus on Lysophosphatidylcholine

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Granulysin binds to phospholipids in cell membranes, which is important for its cytotoxic function as it allows the protein to disrupt the integrity of cell membranes and induce cell death [52,53]. The specific role of PLA1A (IFNG-inducible enzyme) in rejection mechanisms is less clear, but phospholipases can modulate inflammatory responses and immune cell functions by generating lipid mediators [54]. An increase in the activity of phospholipases triggers lipid degradation and subsequently energy metabolism imbalance [55,56].…”

Section: Discussionmentioning

confidence: 99%

Prediction of Acute Cardiac Rejection Based on Gene Expression Profiles

Abdrakhimov,

Kayewa,

Wang

2024

JPM

View full text Add to dashboard Cite

Acute cardiac rejection remains a significant challenge in the post-transplant period, necessitating meticulous monitoring and timely intervention to prevent graft failure. Thus, the goal of the present study was to identify novel biomarkers involved in acute cardiac rejection, paving the way for personalized diagnostic, preventive, and treatment strategies. A total of 809 differentially expressed genes were identified in the GSE150059 dataset. We intersected genes selected by analysis of variance, recursive feature elimination, least absolute shrinkage and selection operator, and random forest classifier to identify the most relevant genes involved in acute cardiac rejection. Thus, HCP5, KLRD1, GZMB, PLA1A, GNLY, and KLRB1 were used to train eight machine learning models: random forest, logistic regression, decision trees, support vector machines, gradient boosting machines, K-nearest neighbors, XGBoost, and neural networks. Models were trained, tested, and validated on the GSE150059 dataset (MMDx-based diagnosis of rejection). Eight algorithms achieved great performance in predicting acute cardiac rejection. However, all machine learning models demonstrated poor performance in two external validation sets that had rejection diagnosis based on histology: merged GSE2596 and GSE4470 dataset and GSE9377 dataset, thus highlighting differences between these two methods. According to SHAP and LIME, KLRD1 and HCP5 were the most impactful genes.

show abstract

“…Additionally, phosphoinositides can also be hydrolyzed by different kinds of phospholipases, producing secondary messengers to regulate immune cell responses [11]. The significance of phospholipases and the production of secondary messengers in the immune system has been well defined in other reviews [11,12]. For the present review, we will be limiting our discussion to the production and conversion of different phosphoinositides by specific kinases and phosphatases during immune cell migration.…”

Section: Introductionmentioning

confidence: 99%

Phosphoinositide Signaling in Immune Cell Migration

Kakar,

Ghosh,

Sun

2023

Biomolecules

View full text Add to dashboard Cite

In response to different immune challenges, immune cells migrate to specific sites in the body, where they perform their functions such as defense against infection, inflammation regulation, antigen recognition, and immune surveillance. Therefore, the migration ability is a fundamental aspect of immune cell function. Phosphoinositide signaling plays critical roles in modulating immune cell migration by controlling cell polarization, cytoskeletal rearrangement, protrusion formation, and uropod contraction. Upon chemoattractant stimulation, specific phosphoinositide kinases and phosphatases control the local phosphoinositide levels to establish polarized phosphoinositide distribution, which recruits phosphoinositide effectors to distinct subcellular locations to facilitate cell migration. In this Special Issue of “Molecular Mechanisms Underlying Cell Adhesion and Migration”, we discuss the significance of phosphoinositide production and conversion by phosphoinositide kinases and phosphatases in the migration of different types of immune cells.

show abstract

Interface of Phospholipase Activity, Immune Cell Function, and Atherosclerosis

Cited by 14 publications

References 153 publications

Phospholipases and Reactive Oxygen Species Derived Lipid Biomarkers in Healthy and Diseased Humans and Animals – A Focus on Lysophosphatidylcholine

Phospholipases and Reactive Oxygen Species Derived Lipid Biomarkers in Healthy and Diseased Humans and Animals – A Focus on Lysophosphatidylcholine

Prediction of Acute Cardiac Rejection Based on Gene Expression Profiles

Phosphoinositide Signaling in Immune Cell Migration

Contact Info

Product

Resources

About