Intérêt du typage moléculaire pour l’épidémiologie de la tuberculose en Limousin (1998–2006)

Bézanahary, H.; Baclet, M; Sola, Christophe; Gazaille, V.; Turlure, Pascal; Weinbreck, P.; Denis, F.; Martin, Clémence

doi:10.1016/j.medmal.2008.02.008

Cited by 6 publications

(6 citation statements)

References 18 publications

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“…They represent as much as 60% of M. tuberculosis strains isolated in Italy (31), 70% of the strains from Sweden (10), and 80% of the strains from France and Portugal (6,11). Thus, the population structure revealed in our study is characteristic of a European country.…”

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confidence: 57%

Spoligotype-Based Comparative Population Structure Analysis of Multidrug-Resistant and Isoniazid-Monoresistant Mycobacterium tuberculosis Complex Clinical Isolates in Poland

Jagielski¹,

Augustynowicz–Kopeć²,

Zozio

et al. 2010

J Clin Microbiol

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The spoligotyping-based population structure of multidrug-resistant (MDR) Mycobacterium tuberculosis strains isolated in Poland (n ‫؍‬ 46), representing all culture-positive MDR tuberculosis (MDR-TB) cases, was compared to that of isoniazid (INH)-monoresistant strains (n ‫؍‬ 71) isolated in 2004. The latter data set from a previous study (E. Augustynowicz-Kopeć, T. Jagielski, and Z. Zwolska, J. Clin. Microbiol. 2008, 46:4041-4044) represented 87% of all INH-monoresistant strains. The clustering rates and genotypic-diversity indexes for the 2 subpopulations were not significantly different (P ‫؍‬ 0.05). The results were entered in the SITVIT2 database to assign specific shared type designations, corresponding genotypic lineages, and geographical distributions and compared to available data from neighboring countries (Germany, n ‫؍‬ 704; Czech Republic, n ‫؍‬ 530; Sweden, n ‫؍‬ 379; Kaliningrad, Russia, n ‫؍‬ 90) and strains from previous studies in Poland (n ‫؍‬ 317). MDR strains resulted in 27 patterns (20 unique strains within the study and 7 clusters containing 2 to 6 isolates per cluster with a clustering rate of 56.5%) and belonged to the following genotypic lineages: ill-defined T family (28.3%), Haarlem (17.4%), Latin American and Mediterranean (LAM) (13%), Beijing (8.7%), S family (4.35%), and the X clade (2.17%). Comparison of the genetic structure of the MDR strains with that of INH-monoresistant strains showed that a total of 9 patterns were shared by both groups; these represented 1/3 of the MDR strains and 2/3 of the INH-monoresistant strains. Interestingly, 76.1% of the MDR isolates and 71.8% of the INH-resistant isolates yielded spoligotypes that were previously reported from Poland. The observation that nearly half of the spoligotypes identified among both MDR (48.1%) and INH-monoresistant (43.3%) M. tuberculosis isolates were present in Poland's neighboring countries suggested that a significant proportion of MDR and INH-resistant TB cases in Poland were caused by strains actively circulating in Poland or its neighbors. Our results corroborate the leading role of the T and Haarlem genotypes in the epidemiology of drug-resistant TB in Poland. Nevertheless, the LAM and Beijing family strains that infected, correspondingly, 13% and 9% of patients with MDR-TB were absent among the strains from patients with INHmonoresistant TB, suggesting that a proportion of MDR-TB cases in Poland are due to ongoing transmission of MDR clones exhibiting specific genotypes. Study of the population genetic relationships between MDR and INH-monoresistant strains by drawing minimum spanning trees showed that ill-defined T1 sublineage strains (1/3 of all INH-monoresistant strains), represented by its prototype, SIT53, constituted the central node of the tree, followed by strains belonging to the well-defined H3, H1, and S subgroups. However, the MDR group, in addition, contained LAM (n ‫؍‬ 6) and Beijing (n ‫؍‬ 4) lineage isolates. With the exception of the 4 Beijing lineage strains in the latter group and a single orpha...

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confidence: 57%

Spoligotype-Based Comparative Population Structure Analysis of Multidrug-Resistant and Isoniazid-Monoresistant Mycobacterium tuberculosis Complex Clinical Isolates in Poland

Jagielski¹,

Augustynowicz–Kopeć²,

Zozio

et al. 2010

J Clin Microbiol

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“…The included studies were performed in Spain (n=5) [23, 28, 30, 33, 34], Italy (n=1) [27], Germany (n=2) [21, 26], Belgium (n=1) [20], France (n=2) [22, 29], UK (n=1) [32], Sweden (n=1) [24], Denmark (n=1) [31], and Norway (n=1) [25] (table 1). …”

Section: Resultsmentioning

confidence: 99%

“…Five studies were hospital-based [20, 22, 27, 30, 32] and 10 were population-based [21, 23–26, 28, 29, 31, 33, 34], performed from 1992 [31] to 2007 [28, 30, 34], with either retrospective [22, 33] or prospective [20, 21, 26–32, 34] study designs. Two studies were nationwide (Denmark [31] and Norway [25]); the other 13 were regional or in an urban area.…”

Section: Resultsmentioning

confidence: 99%

Tuberculosis transmission between foreign- and native-born populations in the EU/EEA: a systematic review

Sandgren

Schepisi

Sotgiu

et al. 2013

European Respiratory Journal

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Tuberculosis (TB) control programmes of many low TB incidence countries of the European Union/European Economic Area (EU/EEA) perceive challenges in controlling TB due to high numbers of TB in migrants from high-incidence countries.To assess the extent of TB transmission from the foreign-born to the native-born population, we quantitatively investigated the dynamics of TB transmission between these populations in the EU/EEA, using published molecular epidemiological studies. We searched PubMed and EMBASE databases from 1990 to August 2012.We identified 15 studies performed during 1992–2007 covering 12,366 cases, of which median (range) 49.2% (17.7%–86.4%) were foreign-born. The proportion of clustered isolates ranged between 8.5% and 49.1% of the total number of TB cases genotyped and among these, foreign-born cases were equally or more likely to have unique isolates compared to native-born cases. One third of the clusters were “mixed”, i.e. composed of foreign- and native-born cases, involving 0–34.2% of all genotyped cases. Cross-transmission among foreign and native populations was bidirectional, with wide differences across studies.This systematic review provides evidence that TB in a foreign-born population does not have a significant influence on TB in the native population in EU/EEA.

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“…Molecular epidemiology has revolutionized the study of tuberculosis (TB) transmission and the management of TB disease (Allix-Beguec et al, 2008; Bezanahary et al, 2008; David, 2008; Fica et al, 2008; Mathema et al, 2008). The fundamental principal behind the molecular epidemiology of TB is that, unlike other bacteria, Mtb replicates clonally and almost never transfers genes horizontally.…”

Section: Introductionmentioning

confidence: 99%

“…This together with the fact that Mtb underwent a genetic bottleneck recently, makes it relatively monomorphic (Gibson et al, 2008). Detectable genetic variation between different Mtb bacilli does evolve over time, however, which means Mtb strains with identical genotypes found in different patients are most likely the result of recent transmission (Allix-Beguec et al, 2008; Bezanahary et al, 2008; David, 2008; Fica et al, 2008; Mathema et al, 2008). Furthermore, Mtb strains with genotypes shown to be closely related by phylogenetic analysis (defined as differing by only one MIRU locus in this study) were probably derived from one another, giving rise to the notion of a clonal complex of Mtb strains that may, but not necessarily do, share a recent transmission event as well (Allix-Beguec et al, 2008; Weniger et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Factors associated with genotype clustering of Mycobacterium tuberculosis isolates in an ethnically diverse region of southern California, United States

Rodwell¹,

Kapasi²,

Barnes³

et al. 2012

Infection, Genetics and Evolution

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Mycobacterium tuberculosis (Mtb) isolates with identical genotypes, found in different patients, are most likely the result of recent transmission. Mtb strains with closely related genotypes, called clonal complexes, are most likely derived from one another. We examined Mtb genotypes from southern California TB patients from 2005 through 2008 to complete the first comprehensive molecular epidemiology analysis of this complicated and ethnically diverse region. Mtb genotypes were characterized with spoligo-type and MIRU-12 typing. MIRU-VNTRplus was utilized to assign genotypes to global lineages and complete cluster analyses. Associations between patient characteristics and genotype clustering and clonal complexes were evaluated using logistic regression and frequency analysis. Of 832 Mtb isolates analyzed, 480 (58%) fell into 94 strain clusters. The majority of isolates were identified as being in the EA1 (31%), LAM (17%) and Haarlem (15%) lineages, but 13 different lineages were found in this region. TB patients with clustered isolates were more likely to be homeless (AOR 3.44, 95% CI 1.65, 7.18) and male (AOR 1.57, 95% CI 1.17, 2.10). Of the 480 clustered strains, 388 aggregated into six clonal complexes. Over 45% of reported TB cases were clustered and likely resulted from recent transmission events. Patients with clustered Mtb isolates that were grouped into clonal complexes had unique socio-demographic characteristics. These data suggest that TB is being transmitted in relatively insular community networks defined by race/ethnicity and country of origin. The addition of clonal complex analysis to simple cluster analysis provides important public health insights into the local transmission of TB in ethnically diverse regions with diverse Mtb genotypes.

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Intérêt du typage moléculaire pour l’épidémiologie de la tuberculose en Limousin (1998–2006)

Cited by 6 publications

References 18 publications

Spoligotype-Based Comparative Population Structure Analysis of Multidrug-Resistant and Isoniazid-Monoresistant Mycobacterium tuberculosis Complex Clinical Isolates in Poland

Spoligotype-Based Comparative Population Structure Analysis of Multidrug-Resistant and Isoniazid-Monoresistant Mycobacterium tuberculosis Complex Clinical Isolates in Poland

Tuberculosis transmission between foreign- and native-born populations in the EU/EEA: a systematic review

Factors associated with genotype clustering of Mycobacterium tuberculosis isolates in an ethnically diverse region of southern California, United States

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