Interest of Human Papillomavirus DNA quantification and genotyping in paired cervical and urine samples to detect cervical lesions

Ducancelle, Alexandra; Legrand, M.-C.; Pivert, Adeline; Veillon, Pascal; Guillou‐Guillemette, Hélène Le; Brux, M.-A. De; Beby‐Defaux, Agnès; Agius, G.; Hantz, Sébastien; Alain, Sophie; Catala, L.; Descamps, Philippe; Postec, E.; Caly, H.; Charles-Pétillon, F.; Labrousse, François; Lunel, F.; Payan, Christopher

doi:10.1007/s00404-014-3191-y

Cited by 20 publications

(29 citation statements)

References 39 publications

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“…Similar outcomes were reported by Ramqvist and colleagues, who found a 62% of HPV prevalence involving sexually active women from Stockholm, Sweden, ages 15 to 23 years and by Wheeler, who found a maximum HPV prevalence of 52% at age 20 years in the general New Mexico population (38,39). These results are consistent with previous observations that indicate that HPV infection rates are higher after sexual initiation and are shown to peak around the age of 25 (31,40).…”

Section: Discussionsupporting

confidence: 90%

“…In our study, the agreement between any HPV type detection in urine and cervical samples was 84.00% with a Cohen's kappa coefficient of 0.60, similar to that reported by Cuschieri and colleagues. In relation to concordance for LR-HPV and HR-HPV types included in tetravalent vaccine, in our study, concordance was substantial for both, k ¼ 0.70 (95% CI, 0.66-0.74) for HPV-6 and -11 and k ¼ 0.76 (95% CI, 0.78-0.84) for HPV-16 and -18 (31,37,41,(43)(44)(45).…”

Section: Discussionmentioning

confidence: 52%

“…However, comparable results have been described by Cuschieri and colleagues (67% vs. 59%; ref. 31), Bernal and colleagues (53% vs. 50%; ref. 41), and Ducancelle and colleagues (59% vs. 54%; ref.…”

Section: Discussionmentioning

confidence: 98%

“…Similarly, urine sampling has been proposed as an adequate alternative for monitoring HPV prevalence in female adolescents in order to determinate the early effect of HPV vaccination in the targeted age cohorts (19,(30)(31)(32)(33). However, the results have not been conclusive due to a number of variables that could negatively affect HPV detection.…”

Section: Discussionmentioning

confidence: 99%

“…41), and Ducancelle and colleagues (59% vs. 54%; ref. 31), among women between 18 and 25 years old. In addition to a higher HPV prevalence in urine, the absolute number of HPV genotypes that were detected in urine samples was higher (up to 9) than the number of genotypes identified in cervical samples.…”

Section: Discussionmentioning

confidence: 99%

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Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia

Cómbita

Gheit

González

et al. 2016

Cancer Prevention Research

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Urine sampling for HPV DNA detection has been proposed as an effective method for monitoring the impact of HPV vaccination programs; however, conflicting results have been reported. The goal of this study was to evaluate the performance of optimized urine HPV DNA testing in women aged 19 to 25 years. Optimization process included the use of first void urine, immediate mixing of urine with DNA preservative, and the concentration of all HPV DNA, including cell-free DNA fragments. Urine and cervical samples were collected from 535 young women attending cervical screening at health centers from two Colombian cities. HPV DNA detection and genotyping was performed using an HPV type-specific multiplex genotyping assay, which combines multiplex polymerase chain reaction with bead-based Luminex technology. Concordance between HPV DNA detection in urine and cervical samples was determined using kappa statistics and McNemar tests. The accuracy of HPV DNA testing in urine samples was evaluated measuring sensitivity and specificity using as reference the results obtained from cervical samples. Statistical analysis was performed using STATA11.2 software. The findings revealed an overall HPV prevalence of 60.00% in cervical samples and 64.72% in urine samples, HPV-16 being the most frequent HPV type detected in both specimens. Moreover, our results indicate that detection of HPV DNA in first void urine provides similar results to those obtained with cervical samples and can be used to monitor HPV vaccination trials and programs as evidenced by the substantial concordance found for the detection of the four vaccine types.Cancer Prev Res; 9(9); 766-71. Ó2016 AACR.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia

Cómbita

Gheit

González

et al. 2016

Cancer Prevention Research

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Detection and genotyping of HPV in urine samples from Chilean women attending primary health care centers

Vergara

Balanda

Hidalgo³

et al. 2017

Med Microbiol Immunol

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Cervical cancer is the second most common malignant neoplasm in women worldwide representing approximately 10% of all types of cancers. Triage of women through cervical cytology has been an important strategy for the surveillance and control of new cases of cervical cancer. However, in many regions around the world cervical cytology has a low coverage compared to developed countries. The molecular detection of HPV is the most effective method to increase the screening sensitivity of women at risk of developing cervical cancer. There are very few studies about the efficacy of urine testing for detection of HPV in women followed up in primary health care centers. Consequently, the efficacy of using urine HPV screening in these populations has not been addressed yet. Here, we compared the detection of HPV in simultaneous urine and cervical samples of women followed up in primary health care centers. Urine and cervical samples were analyzed in 543 women attending at primary health care centers. HPV was detected by real time PCR, and HPV typing performed by PCR-RLB. A general HPV concordance of 86.2% (κ = 0.72) was determined between urine and cervical samples. The concordance for HPV-16 and 18 was almost perfect (κ = 0.82) and strong (κ = 0.77), respectively. The sensitivity and specificity for all HPV genotypes in urine using cervical samples as reference were 82.1 and 93.7%, respectively. The results showed that urine is a good alternative as clinical sample for HPV screening in women attending primary health care centers. Therefore, urine should be used as an alternative sample for increasing triage coverage either in refractory women participating in Pap surveillance programs or when cervical samples are not available.

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Human papillomavirus genotype and viral load agreement between paired first-void urine and clinician-collected cervical samples

Keer

Tjalma

Pattyn

et al. 2018

Eur J Clin Microbiol Infect Dis

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The performance and acceptability of first-void urine as specimen for the detection of HPV DNA in a Belgian referral population was evaluated using an optimized sample collection and processing protocol. One hundred ten first-void urine and cervical samples were collected from 25- to 64-year-old women who were referred for colposcopy (January–November 2016). Paired samples were analyzed by the Riatol qPCR HPV genotyping assay. Acceptability data were gathered through questionnaires (NCT02714127). A higher high-risk HPV DNA prevalence was observed in first-void urine (n = 76/110) compared to cervical samples (n = 73/110), with HPV31 and HPV16/31 being most prevalent correspondingly. For both any and high-risk HPV DNA, good agreement was observed between paired samples (Cohen’s Kappa of 0.660 (95% CI: 0.486–0.833) and 0.688 (95% CI: 0.542–0.835), respectively). In addition, significant positive correlations in HPV copies (per microliter of DNA extract) between paired samples were observed for HPV16 (rs = 0.670; FDR (false discovery rate)-adjusted p = 0.006), HPV18 (rs = 0.893; FDR-adjusted p = 0.031), HPV31 (rs = 0.527; FDR-adjusted p = 0.031), HPV53 (rs = 0.691; FDR-adjusted p = 0.017), and HPV68 (rs = 0.569; FDR-adjusted p = 0.031). First-void urine sampling using a first-void urine collection device was preferred over a clinician-collected cervical sample. And mostly, first-void urine sampling at home was favored over collection at the clinic or the general practitioner’s office. First-void urine sampling is a highly preferred, non-invasive method that ensures good agreement in HPV DNA (copies) with reference cervical samples. It is particularly interesting as a screening technique to reach non-participants, and its clinical performance should be further evaluated.Electronic supplementary materialThe online version of this article (10.1007/s10096-017-3179-1) contains supplementary material, which is available to authorized users.

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Interest of Human Papillomavirus DNA quantification and genotyping in paired cervical and urine samples to detect cervical lesions

Abstract: High concordance rates for HPV-DNA quantification and high/low-risk HPV genotyping in paired urine/cervical samples suggest that urinary HPV DNA testing could be useful for cervical lesion screening.

Cited by 20 publications

References 39 publications

Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia

Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia

Detection and genotyping of HPV in urine samples from Chilean women attending primary health care centers

Human papillomavirus genotype and viral load agreement between paired first-void urine and clinician-collected cervical samples

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