Interdisciplinary gene manipulation, molecular cloning, and recombinant expression of modified human growth hormone isoform-1 in E. coli system

Nasr, Sami Mohamed; Samir, Safia; Okasha, Hend

doi:10.1016/j.ijbiomac.2023.128637

Cited by 1 publication

(1 citation statement)

References 36 publications

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“…In order to meet the requirements for the industrial production of bacteriocin, researchers have attempted to construct cell factories suitable for bacteriocin expression in various hosts. E. coli is a widely used strain since it is easy to culture, has a concise life and is easily manipulated genetically due to its well-known genetics [ 55 ].…”

Section: The Production Of Class Iia Bacteriocins By Different Cell F...mentioning

confidence: 99%

The Progress of the Biotechnological Production of Class IIa Bacteriocins in Various Cell Factories and Its Future Challenges

Wang,

Shang,

Huang

et al. 2024

IJMS

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Class IIa bacteriocins produced in lactic acid bacteria are short cationic peptides with antimicrobial activity. In the search for new biopreservation agents, class IIa bacteriocins are considered to be the best potential candidates, not only due to their large abundance but also because of their high biological activity and excellent thermal stability. However, regulated by the biosynthetic regulatory system, the natural class IIa bacteriocin yield is low, and the extraction process is complicated. The biotechnological production of class IIa bacteriocins in various cell factories has been attempted to improve this situation. In this review, we focus on the application of biotechnological routes for class IIa bacteriocin production. The drawbacks and improvements in the production of class IIa bacteriocins in various cell factories are discussed. Furthermore, we present the main challenge of class IIa bacteriocins, focusing on increasing their production by constructing suitable cell factories. Recombinant bacteriocins have made considerable progress from inclusion body formation, dissolved form and low antibacterial activity to yield recovery. The development of prospective cell factories for the biotechnological production of bacteriocins is still required, which may facilitate the application of bacteriocins in the food industry.

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Section: The Production Of Class Iia Bacteriocins By Different Cell F...mentioning

confidence: 99%

The Progress of the Biotechnological Production of Class IIa Bacteriocins in Various Cell Factories and Its Future Challenges

Wang,

Shang,

Huang

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

Interdisciplinary gene manipulation, molecular cloning, and recombinant expression of modified human growth hormone isoform-1 in E. coli system

Cited by 1 publication

References 36 publications

The Progress of the Biotechnological Production of Class IIa Bacteriocins in Various Cell Factories and Its Future Challenges

The Progress of the Biotechnological Production of Class IIa Bacteriocins in Various Cell Factories and Its Future Challenges

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