Interdigitated hydrocarbon chain packing causes the biphasic transition behavior in lipid/alcohol suspensions

Simon, Sidney A.; McIntosh, Thomas J.

doi:10.1016/0005-2736(84)90562-5

Cited by 307 publications

(239 citation statements)

References 19 publications

Supporting

Mentioning

200

Contrasting

Unclassified

Order By: Relevance

“…This is consistent with experimental results [66]. Since the interdigitated phase is more closely packed than the noninterdigitated phase, the van der Waals energy is greater.…”

Section: Phase Behavior As a Function Of Tail Lengthsupporting

confidence: 92%

“…To study the effect of these molecules on the properties studies on various model membranes have been carried out by various groups [66,[71][72][73][74][75][76]. These studies show that at low temperatures adding alcohol leads the formation of an interdigitated or L βI phase in stead of the commonly formed L c or L β (gel phases).…”

Section: Effect Of Alcoholmentioning

confidence: 99%

“…Our simulations also report the so-called "biphasic effect" on the transition from the low temperature gel phase to the high temperature fluid phase [71]; at low concentrations of alcohol, the main transition temperature shifts to a lower temperature, while at high concentrations this transition temperature shifts to a higher temperature compared to a pure lipid bilayer. Figure 13 shows that the increase or decrease in temperature are directly related to the effect of the alcohol on the stability of the low temperature phases [66]. The L β , phase is destabilized by alcohol, while the L βI can easily incorporate alcohol molecules.…”

Section: Effect Of Alcoholmentioning

confidence: 99%

See 2 more Smart Citations

Mesoscopic Simulations of Biological Membranes

Smit

Kranenburg

Sperotto

et al. 2006

Computer Simulations in Condensed Matter Systems: From Materials to Chemical Biology Volume 2

View full text Add to dashboard Cite

“…This is consistent with experimental results [66]. Since the interdigitated phase is more closely packed than the noninterdigitated phase, the van der Waals energy is greater.…”

Section: Phase Behavior As a Function Of Tail Lengthsupporting

confidence: 92%

Section: Effect Of Alcoholmentioning

confidence: 99%

Section: Effect Of Alcoholmentioning

confidence: 99%

See 1 more Smart Citation

Mesoscopic Simulations of Biological Membranes

Smit

Kranenburg

Sperotto

et al. 2006

Computer Simulations in Condensed Matter Systems: From Materials to Chemical Biology Volume 2

View full text Add to dashboard Cite

“…During this process alcohol, which primarily resides adjacent to the phosphate group (39), decreases the extent of water hydration of the lipid heads and provides greater exposure of the lipid tail; processes that could increase the likelihood of fusion events (72). However, interdigitation is usually only observed in pure PC membranes and at alcohol concentrations higher than 5% v/v (36,72,73). Therefore, interdigitation does not explain the phenomena we observe at lower doses with membranes that contain PC, PE, and cholesterol lipids (see Materials and Methods).…”

Section: Possible Mechanisms For Enhancement Of Fusion By Cis Additiomentioning

confidence: 99%

Drunken Membranes: Short-Chain Alcohols Alter Fusion of Liposomes to Planar Lipid Bilayers

Paxman

Hunt

Hallan

et al. 2017

Biophysical Journal

View full text Add to dashboard Cite

Although the effects of ethanol on protein receptors and lipid membranes have been studied extensively, ethanol's effect on vesicles fusing to lipid bilayers is not known. To determine the effect of alcohols on fusion rates, we utilized the nystatin/ergosterol fusion assay to measure fusion of liposomes to a planar lipid bilayer (BLM). The addition of ethanol excited fusion when applied on the cis (vesicle) side, and inhibited fusion on the trans side. Other short-chain alcohols followed a similar pattern. In general, the inhibitory effect of alcohols (trans) occurs at lower doses than the excitatory (cis) effect, with a decrease of 29% in fusion rates at the legal driving limit of 0.08% (w/v) ethanol (IC 50 ¼ 0.2% v/v, 34 mM). Similar inhibitory effects were observed with methanol, propanol, and butanol, with ethanol being the most potent. Significant variability was observed with different alcohols when applied to the cis side. Ethanol and propanol enhanced fusion, butanol also enhanced fusion but was less potent, and low doses of methanol mildly inhibited fusion. The inhibition by trans addition of alcohols implies that they alter the planar membrane structure and thereby increase the activation energy required for fusion, likely through an increase in membrane fluidity. The cis data are likely a combination of the above effect and a proportionally greater lowering of the vesicle lysis tension and hydration repulsive pressure that combine to enhance fusion. Alternate hypotheses are also discussed. The inhibitory effect of ethanol on liposome-membrane fusion is large enough to provide a possible biophysical explanation of compromised neuronal behavior.

show abstract

“…The "biphasic effect" indicates two independent interactions within different concentration ranges [46,47]. The biphasic effect is most strongly characterized by an initial decrease in the Tm, but an increase in or stabilization of the Tm once the L I phase is formed.…”

Section: Chemically-induced Interdigitation Of Phosphatidylcholinesmentioning

confidence: 99%

Differential Scanning Calorimetry Studies of Phospholipid Membranes: The Interdigitated Gel Phase

Smith¹,

Dea²

2013

Applications of Calorimetry in a Wide Context - Differential Scanning Calorimetry, Isothermal Titration Calorimetry and Microca

View full text Add to dashboard Cite

Interdigitated hydrocarbon chain packing causes the biphasic transition behavior in lipid/alcohol suspensions

Cited by 307 publications

References 19 publications

Mesoscopic Simulations of Biological Membranes

Mesoscopic Simulations of Biological Membranes

Drunken Membranes: Short-Chain Alcohols Alter Fusion of Liposomes to Planar Lipid Bilayers

Differential Scanning Calorimetry Studies of Phospholipid Membranes: The Interdigitated Gel Phase

Contact Info

Product

Resources

About