Interdependence of Ca2+ and proton movements in trout hepatocytes

Ahmed, Khaled; Pelster, Bernd

doi:10.1242/jeb.008862

Cited by 4 publications

(5 citation statements)

References 49 publications

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“…In the present study, steady-state pH L was slightly decreased upon removal of extracellular Ca 2+ . In our previous work (Ahmed and Pelster, 2007), this treatment was not accompanied by a change in cytosolic [Ca 2+ ] for a period of 10 min, except for an initial brief (but high) increase in [Ca 2+ ] i supposedly due to a mechanical effect. If Ca 2+ release from acidic compartments is contributing to this [Ca 2+ ] i increase, then it is possible that Ca 2+ -H + exchange is involved.…”

Section: Involvement Of Ca 2+mentioning

confidence: 67%

“…Interestingly, BAPTA exposure induced an increase in pH L . This alkalinization of acidic compartments might explain the BAPTAinduced pH i decrease measured in these cells (Ahmed and Pelster, 2007). Sequentially adding bafilomycin A1 and BAPTA in a different order suggested that BAPTA increased pH L through activating H + leak pathways.…”

Section: Involvement Of Ca 2+mentioning

confidence: 85%

“…Glucose injection into human tumour cells substantially decreased pH e while pH i remained unchanged (Kozin et al, 2001). Similarly, pH i of fish hepatocytes increased in response to Ca 2+ -mobilizing agents with no change in pH e (Ahmed and Pelster, 2007).…”

Section: Introductionmentioning

confidence: 90%

“…We have shown recently that removal of extracellular Ca 2+ or chelation of intracellular Ca 2+ affects pH i under steady-state conditions (Ahmed and Pelster, 2007) and during hypertonicity challenge (Ahmed et al, 2006). Thus, in the next set of experiments, we attempted to investigate possible changes in pH L in response to the above-mentioned conditions.…”

Section: Ca 2+ Involvementmentioning

confidence: 99%

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Ionic determinants of pH of acidic compartments under hypertonic conditions in trout hepatocytes

Ahmed

Pelster

2008

Journal of Experimental Biology

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SUMMARY 5 mmol l -1 ). Furthermore, hypertonicity-induced acidification was still noticeable in the presence of SITS. On the other hand, the hypertonicity-induced acidification was significantly reduced in the absence of extracellular Na + or Ca 2+ . However, BAPTA-AM induced an increase in steady-state pH L that was independent of V-ATPase inhibition. Moreover, the BAPTA-induced alkalinization was still apparent after depletion of intracellular Ca 2+ using the Ca 2+ ionophore A23187 in Ca 2+ -free medium. We conclude that pH L of trout hepatocytes is sensitive to hypertonicity and ionic determinants of hypertonicity. Thus, changes in pH L should be considered when studying pH adaptations to hypertonic stress.

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Section: Involvement Of Ca 2+mentioning

confidence: 67%

Section: Involvement Of Ca 2+mentioning

confidence: 85%

Section: Introductionmentioning

confidence: 90%

Section: Ca 2+ Involvementmentioning

confidence: 99%

See 2 more Smart Citations

Ionic determinants of pH of acidic compartments under hypertonic conditions in trout hepatocytes

Ahmed

Pelster

2008

Journal of Experimental Biology

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“…It is unclear why the effect of ionomycin on cell death increases at an acidic pH. The intracellular calcium ion concentration was higher in an acidic medium than that in an alkaline medium [31]. The increased cytotoxicity of ionomycin at an acidic pH might be related to the increase in the intracellular calcium ion concentration at an acidic pH.…”

Section: Acidosis-dependent Anti-cancer Drugsmentioning

confidence: 99%

Cancer Chemotherapy Specific to Acidic Nests

Kobayashi

2017

Cancers

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The realization of cancer therapeutics specific to cancer cells with less of an effect on normal tissues is our goal. Many trials have been carried out for this purpose, but this goal is still far from being realized. It was found more than 80 years ago that solid cancer nests are acidified, but in vitro studies under acidic conditions have not been extensively studied. Recently, in vitro experiments under acidic conditions were started and anti-cancer drugs specific to acidic areas have been identified. Many genes have been reported to be expressed at a high level under acidic conditions, and such genes may be potent targets for anti-cancer drugs specific to acidic nests. In this review article, recent in vitro, in vivo, and clinical achievements in anti-cancer drugs with marked efficacy under acidic conditions are summarized, and the clinical use of anti-cancer drugs specific to acidic nests is discussed.

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Effect of hypercapnia on intracellular pH regulation in a rainbow trout hepatoma cell line, RTH 149

et al. 2011

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Fish exposed to elevated water CO(2) experience a rapid increase in blood CO(2) levels (hypercapnia), resulting in acidification of both intra- and extra-cellular compartments. While the mechanisms associated with extracellular pH regulation have been well explored, much less is known about intracellular pH (pH(i)) regulation. There is great interest in developing non-animal models for research. One such model is the rainbow trout hepatoma cell line (RTH 149), which has been used to study a wide range of topics; however, no studies have investigated its potential use in pH(i) regulation. Employing the pH-sensitive fluoroprobe BCECF, the present study examined pH(i) regulation in RTH 149 under normocapnia and during extracellular acidification induced by either elevated CO(2) or 1 M HCl. During exposure to hypercapnia, RTH 149 cells were acidified without recovery as long as the elevated CO(2) was maintained. In addition, rates of pH(i) recovery from NH(4)Cl-induced acidosis were significantly lower in cells exposed to hypercapnia or HCl compared to that in normocapnic cells, indicating that elevated CO(2) indirectly impeded pH(i) recovery through a reduction in pH(e) and/or pH(i). Moreover, pH(i) regulation in RTH 149 was EIPA-sensitive, suggesting that an NHE may be involved. Overall, RTH 149 may have the potential for identifying transporters likely to play a role in pH(i) regulation in fish. However, it should not be used as a complete replacement for in vivo studies, especially to quantify acid-base regulatory ability at whole animal level, since RTH 149 appeared to have enhanced pH(i) recovery rates relative to primary hepatocytes.

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Interdependence of Ca2+ and proton movements in trout hepatocytes

Cited by 4 publications

References 49 publications

Ionic determinants of pH of acidic compartments under hypertonic conditions in trout hepatocytes

Ionic determinants of pH of acidic compartments under hypertonic conditions in trout hepatocytes

Cancer Chemotherapy Specific to Acidic Nests

Effect of hypercapnia on intracellular pH regulation in a rainbow trout hepatoma cell line, RTH 149

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