Intercellular network in articular cartilage of pigs with experimentally induced arthritis

R, Denecke; Fj, Kaup; Meyer, Megan

doi:10.1007/bf00541354

Cited by 12 publications

(5 citation statements)

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“…Information has been published on the structure of articular cartilage in the pig under physiological conditions by Perrin et al (1978), Nakano et al (1979), Wilsman et al (1981) and Bhatnagar et al (1981). Pathological changes in articular cartilage have been investigated by Grondalen (1974a, b, c, d, e, f), , Grondalen and Vangen (1974), Doige (1980), Nakano et al (1982) and Denecke et al (1985). In vitro studies (Burch and Lebowitz 1982;Farnum et al 1984), the effects of nutrition, sex and hormones on the occurrence of cartilage lesions, and biochemical aspects (Simunekand Muir 1972a, b) have also been reported.…”

Section: Discussionmentioning

confidence: 99%

The Ultrastructure of Articular Cartilage in the Prenatal Pig

Horký¹

1989

Acta Vet. Brno

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Horky D.: The Ultrastructure of Articular Cartilage in the PrenatalPig. Acta vet. Brno, 58,1989: 143-174.The articular cartilage of the femoral articulation was studied in 14 porcine foetuses of both &exes at 41, 61 and 94 days after fertilization. The structure of articular chondroblasts and intercellular matter enabled us to distinguish three zones as early as at 41 days. The surface zone contained cells at a low degree of differentiation in structure resembling fibroblasts. The medium zone showed thick bundles of collagen microfibrils penetrating through the cell membrane into the intercellular matter. At 61 days both the chondroblast cytoplasm and the cartilage surface of the medium zone had vesicles, several !-10m in size, bounded by a single membrane. At 91 days after fertilization the cytoplasm of chondroblasts found at the border between the surface and the medium zones revealed collagen microfibrils running through cell memblanes. The fibrils showed signs of periodicity.The surface layer of the cartilage in the period under study was made up largerly of aperiodic fibrils. In some regions below the surface bundles of collagen microfibrils ran parallel to the surface of the articular cartilage. They later became involved into the chondrosynovial membrane. Ultrastructure, porcine articular cartilage, developmentIn the course of articular cartilage development both chondrocytes and intercellular matter are subject to differentiation processes which, in the prenatal period, lead to the formation of articular cartilage followed by further changes in the two components in the neonatal period. The changes in the developing articular cartilage are referred to as the maturation process and are determined genetically, endocrinologically, by nutritional conditions (Silberger and Silberger 1941;Silberger et at. 1961) and, particularly in the postnatal period, by endogenous environmental factors (Ghadially 1978(Ghadially , 1981(Ghadially , 1982Perrin et al. 1978). Changes in morphology of articular cartilage are associated, to a major extent, with age (except for species-related changes). Such changes have been reported for instance in adult mice (Silberger et al. 1976), rabbits (Barnett et al. 1963; Davies et al.

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Section: Discussionmentioning

confidence: 99%

The Ultrastructure of Articular Cartilage in the Prenatal Pig

Horký¹

1989

Acta Vet. Brno

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“…Practical reasons led to investigations into the histological changes associated with osteochondrosis and arthrosis in the pig (Grondalen 1987 a,b, c, d, e,f; Grondalen and Vangen 1974;Doige 1980;Nakano et al 1982;Denecke 1985).…”

Section: Discussionmentioning

confidence: 99%

The Submicroscopic Structure of Articular Cartilage in Swine in the Early Postnatal Period

Horký¹

1991

Acta Vet. Brno

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The study was concerned with articular cartilage of the femoral heads in 4 pigs of both sexes at 2 days after birth. The tissue specimens were observed by light microscopy and by transmission and scanning electron microscopy. The surface layer of the cartilage was made up of spindle-shaped chondrocytes arranged in two rows. The chondrocytes sent out long projections into the surrounding intercellular matter in which pericellular and intercellular matrix were distinguished. The Golgi apparatus was well developed and cytoplasmic inclusions were distinct and numerous. Chondrocytes of the middle layer were oval in shape and measured 9 by 6 pm. They contained large amounts of cytoplasm with the Golgi apparatus distributed into several fields; they were rich in mitochondria, granular endoplasmic reticulum, glycogen and lipid droplets. This layer showed the presence of regular cilia and occasional passage of collagen protofibrils through the cell membrane. A clear distinction between pericellular and intercellular matrix was apparent. The zone between the middle and the deep layers showed transitional type chondrocytes. Cells of the deep layer were made into columns situated perpendicular to the surface; they did not differ from the chondrocytes of the corresponding layer in adult animals. "The cartilage surface at 2 days of age was coated with a chondrosynovial membrane. Below this, about 1 pm deep, collagen fibrils running perpendicular to each other produced a solid surface layer capable of resisting to pressure and facilitating movement of the joint.

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“…10,11 A link between decreasing HA molecular weight and reduced tissue mechanical stiffness (otherwise referred to as cartilage 'softening'), associated with damage and microstructural transformations of the collagen fibril network and the onset and progression of osteoarthritis, is well established. [12][13][14] However, it is unclear whether these changes are the cause or the effect of the disease. The etiology of arthritis has proven difficult to investigate experimentally for several reasons.…”

Section: Introductionmentioning

confidence: 99%

Hyaluronic acid–collagen network interactions during the dynamic compression and recovery of cartilage

et al. 2012

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A compression cell designed to fit inside an NMR spectrometer was used to investigate (i) the in situ dynamic strain response and structural changes of the internal pore network, and (ii) the diffusion and flow of interstitial water, in full thickness cartilage samples as they were mechanically deformed under a constant compressive load (pressure) and then allowed to recover (swell again) when the load was removed. Selective enzymatic digestion of the collagen fibril network and the glycopolysaccharide hyaluronic acid (HA) was performed to mimic some of the structural and compositional changes associated with osteoarthritis. Digestion of collagen gave rise to mechanical 'dynamic softening' and-perhaps more importantly-nearly complete loss in the ability to recover through swelling, both effects due to the disruption of the hierarchical structure and fibril interconnectivity in the collagen network which adversely affects its ability to deform reversibly and to properly regulate the pressurization and resulting rate and direction of interstitial fluid flow. In contrast, digestion of HA inside the collagen pore network caused the cartilage to 'dynamically stiffen' which is attributed to the decrease in the osmotic (entropic) pressure of the digested HA molecules confined in the cartilage pores that causes the network to contract and thereby become less permeable to flow. These digestioninduced changes in cartilage's properties reveal a complex relationship between the molecular weight and concentration of the HA in the interstitial fluid, and the structure and properties of the collagen fibril pore network, and provide new insights into how changes in either could influence the onset and progression of osteoarthritis.

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Intercellular network in articular cartilage of pigs with experimentally induced arthritis

Cited by 12 publications

References 13 publications

The Ultrastructure of Articular Cartilage in the Prenatal Pig

The Ultrastructure of Articular Cartilage in the Prenatal Pig

The Submicroscopic Structure of Articular Cartilage in Swine in the Early Postnatal Period

Hyaluronic acid–collagen network interactions during the dynamic compression and recovery of cartilage

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