Intercellular Communication in Response to Radiation Induced Stress: Bystander Effects in Vitro and in Vivo and Their Possible Clinical Implications

Wideł, Maria

doi:10.5772/22584

Cited by 5 publications

(7 citation statements)

References 132 publications

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“…This was less evident when we used conventional dose fractionation (3 × 2 Gy) vs single dose (6 Gy). However, both fractionation schemes were much more effective in inducing apoptosis, especially in bystander cells, than single dose irradiation [38]. The results are in accordance with those presented by others [39], who studied the effects of dose fractionation on RIBE in a keratinocyte cell line and found that the fractionated dose was more toxic than the single dose and was comparable for 2.5 Gy and 1.5 Gy fraction doses.…”

Section: Bystander Effect Induced By Fractionated Irradiationsupporting

confidence: 90%

Radiation Induced Bystander Effect: From <i>in Vitro</i> Studies to Clinical Application

Wideł

2016

IJMPCERO

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In the past 20 years, the classic paradigm in radiobiology recognizing DNA as the main target for the action of radiation has changed. The new paradigm assumes that both targeted and non-targeted effects of radiation determine the final outcome of irradiation. Radiotherapy is one of the main modality treatments of neoplastic diseases with intent to cure, or sometimes to palliate only, thus radiation-induced non-targeted effect, commonly referred to as the radiation-induced bystander effect (RIBE) may have a share in cancer treatment. RIBE is mediated by molecular signaling from radiation targeted cells to their non-irradiated neighbors, and comprises such phenomena as bystander effect, genomic instability, adaptive response and abscopal effect. Whereas first three phenomena may appear both in vitro and in vivo, an abscopal effect is closely related to partial body irradiation and is a systemic effect mediated by immunologic system which synergizes with radiotherapy. From the clinical point of view abscopal effect is particularly interesting due to both its possible valuable contribution to the treatment of metastases, and the potential harmful effects as induction of genetic instability and carcinogenesis. This review summarized the main results of investigations of non-targeted effects coming from in vitro monolayer cultures, 3-dimentional models of tissues, preclinical studies on rodents and clinically observed beneficial abscopal effects with particular emphasis on participation of immunotherapy in the creation of abscopal effects.

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Section: Bystander Effect Induced By Fractionated Irradiationsupporting

confidence: 90%

Radiation Induced Bystander Effect: From <i>in Vitro</i> Studies to Clinical Application

Wideł

2016

IJMPCERO

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“…The radiation‐induced bystander effect (RIBE) is a phenomenon in which nonirradiated cells exhibit some biological changes, usually resembling those in directly irradiated cells. Although RIBE induced by ionizing radiation has been extensively studied , this effect induced by other stressors, among them UV radiation, is relatively limited but it arouses increasing interest in recent years . The solar radiation, which is the main source of UV, comprises three different bands: UVA (320–400 nm), UVB (290–320 nm) and UVC (200–290 nm).…”

Section: Introductionmentioning

confidence: 99%

Bystander Me45 Melanoma Cells Increase Damaging Effect in UVC‐irradiated Cells

Krzywon

Wideł

2019

Photochem & Photobiology

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The aim of our study was to investigate the possible mechanism(s) of the bystander effect induced by UVC light in malignant melanoma Me45 cells that were co-incubated with irradiated cells of the same line. We have found that the UVC band effectively generated apoptosis, premature senescence, single and double DNA strand breaks and reduced clonogenic survival of bystander cells. However, in the feedback response, the bystander cells intensified damage in directly irradiated cells, especially seen at the level of apoptosis and survival of clonogenic cells. Pretreatment of bystander cells with inhibitor of inducible nitric oxide synthase blocks this signaling. It seems that the mediators of this phenomenon produced and secreted by neighboring cells are superoxide, nitric oxide and TGF-b. The reverse deleterious effect caused by cells not exposed to UVC in directly exposed cells is opposed to the protective/rescue effect exerted by the bystander cells in the case of ionizing radiation known in the literature. Whether this opposite adverse effect is a feature of only Me45 melanoma cells or whether it is a general phenomenon occurring between cells of other types exposed to ultraviolet radiation requires further research.

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“…On one hand, it has the potential for killing tumor cells, and on the other hand it causes some damage to normal tissues. [ 8 9 ] In most radiotherapy modalities, fractionated dose regimens are applied,[ 1 ] which would allow the healthy tissue to repair the induced damage between the exposures. This can lead to improved therapeutic ratio; however it is not yet clear if fractionation enhances or decreases the detrimental effects of RIBE in normal tissues.…”

Section: Introductionmentioning

confidence: 99%

“…This can lead to improved therapeutic ratio; however it is not yet clear if fractionation enhances or decreases the detrimental effects of RIBE in normal tissues. Based on our knowledge, in most RIBE studies, acute irradiation has been performed[ 3 8 10 11 12 13 ] and hence little information describing the bystander effects of fractionated irradiation is available. Some authors have pointed out the lack of information in this field and have emphasized on the need for further studies on this topic.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro

Soleymanifard¹,

Toossi²,

Samani³

et al. 2014

J Med Phys

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Radiation-induced bystander effect (RIBE) has been defined as radiation responses observed in nonirradiated cells. It has been the focus of investigators worldwide due to the deleterious effects it induces in nonirradiated cells. The present study was performed to investigate whether acute or fractionated irradiation will evoke a differential bystander response in MRC5 cells. A normal human cell line (MRC5), and a human lung tumor cell line (QU-DB) were exposed to 0, 1, 2, and 4Gy of single acute or fractionated irradiation of equal fractions with a gap of 6 h. The MRC5 cells were supplemented with the media of irradiated cells and their micronucleus frequency was determined. The micronucleus frequency after single and fractionated irradiation did not vary significantly in the MRC5 cells conditioned with autologous or QU-DB cell-irradiated media, except for 4Gy where the frequency of micronucleated cells was lower in those MRC5 cells cultured in the media of QU-DB-exposed with a single dose of 4Gy. Our study demonstrates that the radiation-induced bystander effect was almost similar after single acute and fractionated exposure in MRC5 cells.

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Intercellular Communication in Response to Radiation Induced Stress: Bystander Effects in Vitro and in Vivo and Their Possible Clinical Implications

Cited by 5 publications

References 132 publications

Radiation Induced Bystander Effect: From <i>in Vitro</i> Studies to Clinical Application

Radiation Induced Bystander Effect: From <i>in Vitro</i> Studies to Clinical Application

Bystander Me45 Melanoma Cells Increase Damaging Effect in UVC‐irradiated Cells

Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro

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Intercellular Communication in Response to Radiation Induced Stress: Bystander Effects in Vitro and in Vivo and Their Possible Clinical Implications

Cited by 5 publications

References 132 publications

Radiation Induced Bystander Effect: From &lt;i&gt;in Vitro&lt;/i&gt; Studies to Clinical Application

Radiation Induced Bystander Effect: From &lt;i&gt;in Vitro&lt;/i&gt; Studies to Clinical Application

Bystander Me45 Melanoma Cells Increase Damaging Effect in UVC‐irradiated Cells

Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro

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Radiation Induced Bystander Effect: From <i>in Vitro</i> Studies to Clinical Application

Radiation Induced Bystander Effect: From <i>in Vitro</i> Studies to Clinical Application