Intercellular communication between ciliated cells in culture

Sanderson, Michael J.; Chow, Ida; Dirksen, Ellen R.

doi:10.1152/ajpcell.1988.254.1.c63

Cited by 104 publications

(47 citation statements)

References 15 publications

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“…One important and relevant distinction between this result and the contrary result reported by Sanderson et al 43 is the magnitude of applied force; Sanderson's work reported pushing a micropipette 6 m into the cell membrane, while the deformation reported here is substantially less.…”

Section: Physical Contact Of a Trapped Particle Against The Cell Membcontrasting

confidence: 89%

Use of optical tweezers to probe epithelial mechanosensation

Resnick

2010

J. Biomed. Opt

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Abstract. Cellular mechanosensation mechanisms have been implicated in a variety of disease states. Specifically in renal tubules, the primary cilium and associated mechanosensitive ion channels are hypothesized to play a role in water and salt homeostasis, with relevant disease states including polycystic kidney disease and hypertension. Previous experiments investigating ciliary-mediated cellular mechanosensation have used either fluid flow chambers or micropipetting to elicit a biological response. The interpretation of these experiments in terms of the "ciliary hypothesis" has been difficult due the spatially distributed nature of the mechanical disturbance-several competing hypotheses regarding possible roles of primary cilium, glycocalyx, microvilli, cell junctions, and actin cytoskeleton exist. I report initial data using optical tweezers to manipulate individual primary cilia in an attempt to elicit a mechanotransduction response-specifically, the release of intracellular calcium. The advantage of using laser tweezers over previous work is that the applied disturbance is highly localized. I find that stimulation of a primary cilium elicits a response, while stimulation of the apical surface membrane does not. These results lend support to the hypothesis that the primary cilium mediates transduction of mechanical strain into a biochemical response in renal epithelia.

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Section: Physical Contact Of a Trapped Particle Against The Cell Membcontrasting

confidence: 89%

Use of optical tweezers to probe epithelial mechanosensation

Resnick

2010

J. Biomed. Opt

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“…In chromaffin cells, the problem is that pretreatment with neomycin, a drug that blocks IP3 generation, fails to inhibit spontaneous or caffeine-evoked oscillations although it blocks agonist-evoked ones (70). In hepatocytes, the difficulty is that pretreatment with t-butylhydroperoxide induces typical, periodic 25-Mm/sec calcium waves without inducing formation of any inositol polyphosphate (71 (41). This paper argues that the mechanism of calcium oscillations has been highly conserved; however, evolution is an …”

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confidence: 99%

The path of calcium in cytosolic calcium oscillations: a unifying hypothesis.

Jaffe

1991

Proc. Natl. Acad. Sci. U.S.A.

220

115

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Data from 42 systems have been assembled in which the overall spatial course of relatively natural, intracellular calcium pulses has been or can be determined. These include 21 cases ofsolitary pulses in activating eggs and 21 cases of periodic (as well as solitary) pulses in various fully active cells. In all cases, these pulses prove to be waves of elevated calcium that travel from one pole of a cell to the other or from the periphery inward. The velocities of these waves are remarkably conserved-at -10 pm/sec in activating eggs and -25 pxm/sec in other cells at room temperature. Moreover, in three cases, the data suffice to show that these velocities fit the Luther equation for a reaction/diffusion wave of calcium through the cytosol. It is proposed that (i) natural intracellular calcium pulses quite generally take the form of cytosolic calcium waves and (ii) cytoplasmically controlled calcium waves are triggered and then propagated by the successive action of two distinct modes of calcium-induced calcium release. First, in the lumenal mode, a slow increase of calcium within the lumen of the endoplasmic reticulum reaches a level that triggers fast lumenal release as well as fast localized release into the cytosol. Then, the well-known cytosolic mode drives a reaction/diffusion wave across or into the cell.

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“…All the physiotherapy interventions, with the exception of PEP, had an oscillation frequency within the 3-17 Hz range shown to facilitate mucociliary clearance [4]. These oscillation frequencies may assist secretion clearance by altering rheology of the mucus [4] and increasing ciliary beat through stimulation of the ciliated epithelial cells [24,25]. The oscillation frequencies of Flutter1 (11.3 Hz) and Acapella1 (13.5 Hz) were close to the cited optimal frequency for secretion clearance (13 Hz) [4,13].…”

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confidence: 99%

Physiological effects of vibration in subjects with cystic fibrosis

McCarren

Alison²

2006

European Respiratory Journal

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The physiological mechanisms by which vibration and other physiotherapy interventions may clear secretions in subjects with cystic fibrosis are unknown. The main aim of this study was to compare the expiratory flow rates and frequencies of airflow oscillation of vibration to those of Acapella1, Flutter1, positive expiratory pressure and percussion.Respiratory flow rates were measured during interventions, the order of which was randomised. The oscillation of the airflow of the interventions was determined by frequency spectral analysis.In 18 young adult subjects with cystic fibrosis, the mean peak expiratory flow rate of vibration was greater than Flutter1, percussion, Acapella1 and positive expiratory pressure. The mean¡SD of the oscillation of the airflow of vibration (8.4¡0.4 Hz) was lower than Acapella1 (13.5¡1.7 Hz) and Flutter1 (11.3¡1.5 Hz) but similar to percussion (7.3¡0.3 Hz).Theoretically, the higher peak expiratory flow rate of vibration compared to the other physiotherapy interventions may promote secretion clearance. In addition, the frequency of oscillation of vibration was within the range demonstrated to increase mucus transport. This study has provided some evidence for the physiological rationale for the use of vibration to aid secretion clearance.

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Intercellular communication between ciliated cells in culture

Cited by 104 publications

References 15 publications

Use of optical tweezers to probe epithelial mechanosensation

Use of optical tweezers to probe epithelial mechanosensation

The path of calcium in cytosolic calcium oscillations: a unifying hypothesis.

Physiological effects of vibration in subjects with cystic fibrosis

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