Intercellular Adhesion Molecule-5 Induces Dendritic Outgrowth by Homophilic Adhesion

Tian, Li; Nyman, Henrietta; Kilgannon, Patrick; Yoshihara, Yoshihiro; Mori, Kensaku; Andersson, Leif C.; Kaukinen, Sami; Rauvala, Heikki; Gallatin, W. Michael; Gahmberg, Carl G.

doi:10.1083/jcb.150.1.243

Cited by 47 publications

(59 citation statements)

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“…The expression of ICAM5 is restricted to the mammalian brain telencephalon (24) but there is no homolog in invertebrates and lower vertebrates. The developmental appearance of ICAM5 parallels the time of dendritic elongation, branching, and synapse formation and ICAM5 is described to selectively target dendrites (25) to endow their protrusions with plastic properties (26).In this work we show that an ICAM5 fusion protein has no detectable effect on neuronal morphology and function and that it is specifically and highly enriched in the somatodendritic compartment of both peripheral nervous system (PNS) and CNS neurons. Next, we use this marker to gain insights into dendrite development, identify dendritic compartments, and map neuronal connections.…”

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confidence: 99%

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Genetically encoded dendritic marker sheds light on neuronal connectivity inDrosophila

Nicolaï

Ramaekers

Raemaekers

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

253

227

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In recent years, Drosophila melanogaster has emerged as a powerful model for neuronal circuit development, pathology, and function. A major impediment to these studies has been the lack of a genetically encoded, specific, universal, and phenotypically neutral marker of the somatodendritic compartment. We have developed such a marker and show that it is effective and specific in all neuronal populations tested in the peripheral and central nervous system. The marker, which we name DenMark (Dendritic Marker), is a hybrid protein of the mouse protein ICAM5/Telencephalin and the red fluorescent protein mCherry. We show that DenMark is a powerful tool for revealing novel aspects of the neuroanatomy of developing dendrites, identifying previously unknown dendritic arbors, and elucidating neuronal connectivity.T o discover neuronal circuit architecture, genetic tools that specifically mark the pre-and postsynaptic cells and compartments are necessary. Drosophila is a leading genetic model organism in this regard; however, most neuronal circuits remain unmapped. Of particular note is the lack of a universal, phenotypically neutral, and specific marker of the somatodendritic and postsynaptic compartments. Several molecular differences between dendrites and axons, including the presence of different membrane and cytoskeletal proteins in neuronal subregions, have been identified (1, 2). Drosophila neurons exhibit the major kinds of compartmentalization present in mammalian neurons and the fly has emerged as a powerful system to study the establishment and maintenance of neuronal connections (3, 4). Almost all studies of neuronal circuits in the fly have relied on genetic markers such as CD8::GFP that outline the morphology of entire cells rather than particular subcellular compartments (5), as well as presynaptic markers such as Synaptotagmin, Synaptobrevin, and Bruchpilot GFP fusion proteins (6-11). However, more accurate identification and mapping of novel neuronal circuits has been hampered by the lack of a genetically encoded and phenotypically neutral dendritic marker. Over the years, many such markers have been proposed and several were recently examined (12), namely MAP2 (13, 14), Nod::YFP (4, 15-18), Homer::GFP (19), and DSCAM17.1::GFP (20, 21). The analysis of these markers reveals that none of them labels the entire somatodendritic field. Furthermore, it remains unclear whether the markers tested are neutral with respect to dendritic morphology.Intercellular adhesion molecules (ICAMs) mediate neuronal migration, axon elongation, and fasciculation, synaptogenesis, and synaptic plasticity (22). ICAM5, or Telencephalin, is a 130-kDa type I transmembrane glycoprotein comprising a characteristic extracellular domain, a single transmembrane region, and a short cytoplasmic region (23). The expression of ICAM5 is restricted to the mammalian brain telencephalon (24) but there is no homolog in invertebrates and lower vertebrates. The developmental appearance of ICAM5 parallels the time of dendritic elongation, branching, a...

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confidence: 82%

mentioning

confidence: 99%

Genetically encoded dendritic marker sheds light on neuronal connectivity inDrosophila

Nicolaï

Ramaekers

Raemaekers

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

253

227

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“…ICAM4 has been reported to be exclusively expressed in erythrocytes and has a suggested role in cell interaction events, including hemostasis and thrombosis (33). ICAM5 is mainly expressed in specific areas of the brain (34) and has been implicated in dendritic outgrowth (35) and rapid cell spreading of microglia (36). Although the reported expression patterns and described functions of ICAM4 and ICAM5 are not indicative of a role in breast and prostate cancer susceptibility, their roles in cell adhesion and cell signaling together with their low level expression in cancer-relevant tissues leave the possibility that their dysregulation or dysfunction may increase cancer risk.…”

Section: Discussionmentioning

confidence: 99%

Large-Scale Association Study Identifies ICAM Gene Region as Breast and Prostate Cancer Susceptibility Locus

et al. 2004

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We conducted a large-scale association study to identify genes that influence nonfamilial breast cancer risk using a collection of German cases and matched controls and >25,000 single nucleotide polymorphisms located within 16,000 genes. One of the candidate loci identified was located on chromosome 19p13.2 [odds ratio (OR) ‫؍‬ 1.5, P ‫؍‬ 0.001]. The effect was substantially stronger in the subset of cases with reported family history of breast cancer (OR ‫؍‬ 3.4, P ‫؍‬ 0.001). The finding was subsequently replicated in two independent collections (combined OR ‫؍‬ 1.4, P < 0.001) and was also associated with predisposition to prostate cancer in an independent sample set of prostate cancer cases and matched controls (OR ‫؍‬ 1.4, P ‫؍‬ 0.002). High-density single nucleotide polymorphism mapping showed that the extent of association spans 20 kb and includes the intercellular adhesion molecule genes ICAM1, ICAM4, and ICAM5. Although genetic variants in ICAM5 showed the strongest association with disease status, ICAM1 is expressed at highest levels in normal and tumor breast tissue. A variant in ICAM5 was also associated with disease progression and prognosis. Because ICAMs are suitable targets for antibodies and small molecules, these findings may not only provide diagnostic and prognostic markers but also new therapeutic opportunities in breast and prostate cancer.

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“…TLCN has a homophilic binding activity (Tian et al, 2000b). The homophilic adhesion of TLCN mediates induction of dendritic outgrowth (Tamada et al, 1998).…”

Section: A Transgenic Mouse System For Analysis Of Polarized Targetinmentioning

confidence: 99%

A Novel Phenylalanine-Based Targeting Signal Directs Telencephalin to Neuronal Dendrites

Mitsui

Saito²,

Hayashi³

et al. 2005

J. Neurosci.

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Intercellular Adhesion Molecule-5 Induces Dendritic Outgrowth by Homophilic Adhesion

Cited by 47 publications

References 41 publications

Genetically encoded dendritic marker sheds light on neuronal connectivity inDrosophila

Genetically encoded dendritic marker sheds light on neuronal connectivity inDrosophila

Large-Scale Association Study Identifies ICAM Gene Region as Breast and Prostate Cancer Susceptibility Locus

A Novel Phenylalanine-Based Targeting Signal Directs Telencephalin to Neuronal Dendrites

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