Intercapillary distance measurement as an indicator of hypoxia in carcinoma of the cervix uteri

Awwad, Hassan K.; Aggar, Mervat El; Mocktar, Nadia; Barsoum, Mohsen Samy

doi:10.1016/0360-3016(86)90165-3

Cited by 114 publications

(54 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This result con rms our earlier data (1) and is in agreement with other authors' results (3,10,28), although it contradicts the results of other studies (27). Pretreatment haemoglobin level was not correlated with tumour stage, which is in agreement with other studies (9). However, in the study by Tsang et al (3), patients with smaller tumours showed a signi cantly higher level of haemoglobin.…”

Section: Discussionsupporting

confidence: 90%

“…For example, vascular density (i.e. the proportion of blood vessels in the stroma) (8), inter-capillary distance (ICD) (8,9), intratumour microvessel density (IMD) counts performed on histological cross-sections from a tumour stained with anti-factor VIII (10, 11) and percentage of endothelial cells after Trichrome staining (12). The nitroheterocyclic hypoxia marker EF5, detected by uorescent monoclonal antibodies to the drug adducts (13), or the VEGF mRNA level (14,15) is also applied.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Prognostic Significance of Intratumour Microvessel Density and Haemoglobin Level in Carcinoma of the Uterine Cervix

et al. 2002

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 90%

mentioning

confidence: 99%

Prognostic Significance of Intratumour Microvessel Density and Haemoglobin Level in Carcinoma of the Uterine Cervix

et al. 2002

View full text Add to dashboard Cite

“…limited hypoxia predominates in the primary tumors, and is not related to metastatic efficiency, as opposed to the observed effect of acute hypoxia treatment. The lack of a correlation between CD31 staining and metastasis is consistent with the clinical data, where there is no relationship between vascular density and the presence of lymph node metastasis before treatment, whether the hot spot method is used or whether global parameters of vascularity, such as those used in this study, are used (20,22,23,(25)(26)(27). However, it was reported recently that, in human cervical carcinoma, global intercapillary distance correlates with hypoxia, whereas in the present study, there was no relationship between the EF5-positive fraction and the relative vascular area (26).…”

Section: Discussionsupporting

confidence: 84%

Acute Hypoxia Enhances Spontaneous Lymph Node Metastasis in an Orthotopic Murine Model of Human Cervical Carcinoma

2004

View full text Add to dashboard Cite

An orthotopic mouse model of cervical carcinoma has been used to investigate the relationship between acute (cyclic) hypoxia and spontaneous lymph node metastasis in vivo. The human cervical carcinoma cell line ME-180 was stably transfected to express the fluorescent protein DsRed2, which allowed the in vivo optical monitoring of tumor growth and metastasis by fluorescent microscopy. The surgically implanted primary tumors metastasize initially to local lymph nodes and later to lung, a pattern consistent with the clinical course of the disease. The effect of acute hypoxia on the growth and spread of these tumors was examined by exposing tumor-bearing mice to treatment consisting of exposure to 12 cycles of 10 min 7% O 2 followed by 10 min air (total 4 h) daily during tumor growth. After 21 days, the tumors were excised, lymph node and lung metastases were quantified, and the hypoxic fraction and relative vascular area of the primary tumors were assessed by immunohistochemical staining for the hypoxic marker drug EF5 [2-(2-nitro-1H-imidazole-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide] and the vascular marker CD31, respectively. In untreated mice, the primary tumor size was directly correlated with lymph node metastatic burden. The acute hypoxia treatment resulted in a significant decrease in the size of the primary tumors at the time of excision. However, the mice in the acute hypoxia group had an increased number of positive lymph nodes (2-4) as compared with control mice (1-3). Lung metastasis was not affected. The acute hypoxia treatment also decreased the relative vascular area in the primary tumors but did not affect the hypoxic fraction. These results suggest that fluctuating oxygenation in cervical carcinoma tumors may reduce tumor growth rate, but it may also enhance the ability of tumor cells to metastasize to local lymph nodes.

show abstract

“…Angiogenesis is generally assessed as tumour vascularity, and in carcinoma of the cervix a number of different measurement techniques have been used. Older studies used inter-capillary distance (Kolstad, 1968;Awwad et al, 1986) or percentage of endothelial cells (Siracka et al, 1982) and found that low-vascular density was significantly associated with poor outcome. However, a recent consensus statement proposed the intra-tumour microvessel density (IMD) ('hot-spot') technique as the method of choice for measuring angiogenesis (Vermeulen et al, 1996).…”

mentioning

confidence: 99%

Tumour vascularity is a significant prognostic factor for cervix carcinoma treated with radiotherapy: Independence from tumour radiosensitivity

et al. 1999

View full text Add to dashboard Cite

SummaryThe aim of the study was to investigate the relationship between intrinsic radiosensitivity and vascularity in carcinoma of the cervix given radiotherapy, and assess whether more refined prognostic information can be gained by combining the two parameters. A retrospective study was carried out on 74 patients with locally advanced carcinoma of the cervix. Formalin-fixed, paraffin-embedded tumour biopsies were stained with anti-factor VIII using immunohistochemistry. Vascularity was scored using the intra-tumour microvessel density (IMD), or 'hotspot', technique. For the same patients, the measurement of intrinsic radiosensitivity (SF2) had been made previously on the same pretherapy samples. Patients were stratified by the median IMD and SF2 scores. Women with radioresistant and highly vascular tumours had poorer 5-year survival (P = 0.0005, P = 0.035 respectively) and local control (P = 0.012, P = 0.077 respectively) than those with radiosensitive and poorly vascular tumours. No significant correlation was seen between IMD and SF2. Multivariate analysis (including tumour stage and patient age) showed that only SF2 and IMD were significant prognostic factors for survival. Patients with both a radioresistant and highly vascular tumour had a 5-year survival level of 18% compared to 77% for those patients with a radiosensitive and poorly vascularized tumour. Tumour angiogenesis and cellular radiosensitivity are independent prognostic factors for cervix carcinoma treated with radiotherapy. Allowing for tumour radiosensitivity increases the prognostic significance of vascularity measurements in cervix tumours.

show abstract

Intercapillary distance measurement as an indicator of hypoxia in carcinoma of the cervix uteri

Cited by 114 publications

References 14 publications

Prognostic Significance of Intratumour Microvessel Density and Haemoglobin Level in Carcinoma of the Uterine Cervix

Prognostic Significance of Intratumour Microvessel Density and Haemoglobin Level in Carcinoma of the Uterine Cervix

Acute Hypoxia Enhances Spontaneous Lymph Node Metastasis in an Orthotopic Murine Model of Human Cervical Carcinoma

Tumour vascularity is a significant prognostic factor for cervix carcinoma treated with radiotherapy: Independence from tumour radiosensitivity

Contact Info

Product

Resources

About