Interactive Web Application as a Teaching Tool to Introduce Basics of Chromatography and the Plate Theory

Jordheim, Lars Petter; Denuzière, Anne; Machon, Christelle; Zeinyeh, Waël

doi:10.1021/acs.jchemed.1c00314

Cited by 4 publications

(6 citation statements)

References 5 publications

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“…7 Recently, a free web application was shown to be a useful teaching tool to introduce the plate theory of chromatography to undergraduate students. 8 Microsoft Excel spreadsheets can simulate the process of separation optimization under isocratic and gradient conditions with easily implemented macros; this challenging pedagogical tool is suitable for advanced analytical chemistry courses. 9 Virtual laboratory exercises were proposed at the undergraduate analytical chemistry curriculum level.…”

Section: ■ Introductionmentioning

confidence: 99%

Acting Chromatography

Cecchi

2024

J. Chem. Educ.

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Chromatography is the most common analytical technique able to identify and quantify a wide gamut of substances in food, environmental, forensic, pharmaceutical, and many other kinds of real samples. We propose a simple drama science activity: students embody different compounds wearing emblems of different colors and dramatize their chromatographic separation. They cross the same path at the same speed when they walk, thereby mimicking the fate of molecules in the mobile phase; every now and then, they sit for different times on the chairs along the path, mimicking the adsorption process onto the active sites of the chromatographic bed; the average dwell time is different for different compounds. In this way, students get separated into different groups as they move along the path representing the chromatographic column; as they cross the exit, they trigger musicians to act as a detector: the artists actually play notes (signals) of different pitches and loudness, respectively, for different impersonated substances and amounts. The proposed gamification is appropriate for introductory high school chemistry, and it was carried out within the Content and Language Integrated Learning (CLIL) framework. Still, it can also be played by children (elementary or middle school) in outreach events if math implications are not dealt with. The learning objectives are (i) to make students aware of the chromatography principles and the mechanism the molecules undergo during their chromatographic run, (ii) to make the students explore the influence of experimental parameters on the chromatographic outcome, and (iii) to prevent common misconceptions about the causes of the chromatographic separation. Acting chromatography does not claim to reproduce the reality; it stimulates students to figure out microscopic facets of this macroscopic analytical technique that deserve proper attention. Videomaking is highly appreciated by digital native students; stage performances during outreach events are service-learning aspects involved in this educational activity, which bring about positive emotional energy and stimulate greater engagement.

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Section: ■ Introductionmentioning

confidence: 99%

Acting Chromatography

Cecchi

2024

J. Chem. Educ.

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“…Undergraduate information in High Performance Liquid Chromatography (HPLC) is often limited to basic theory. Expensive HPLC instrumentation, solvents, and related accessories make the practice of this technique prohibitive in many departments; computer HPLC simulation programs − could, therefore, be an aid in teaching the technique. These programs are based on theoretical models with specific compounds that follow the models in such a way that the programs faithfully reproduce the chromatographic behavior observed in experimental HPLC.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Chromatographic Peaks with Excel

Villalon¹

2023

J. Chem. Educ.

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A Spreadsheet Excel application for chromatographic peak characterization is described in this article. Data are exported from HPLC specific program to an Excel file with macros to perform basic calculations as retention time, height, and area of the peaks. From these values, peak resolution, asymmetry, or theoretical plates are calculated and presented in a clear and direct mode. The application may be used as a teaching tool of chromatographic concepts when no HPLC equipment is available. Besides the education purpose, the application may be used for accurate measurement of peak resolution or theoretical plate number by non-usual methods when these features are not included in commercial peak integration software

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“…Several scientific publications have already proposed software [ 4 ] or Excel spreadsheets [ 5 – 7 ] as tools to simulate chromatographic separations. Some are commercial, for example, Drylab (Molnar-Institut, Germany) and ChromSword (Iris Tech) [ 8 ], which may not be compatible with the budget of a state university.…”

Section: Introductionmentioning

confidence: 99%

“…-Opportunity for each student to modify analytical parameters and thus optimize analysis in a faster way -No exposure to chemicals -Minimal wear and tear on instrumentation -Useful for online analytical chemistry education -Save money Several scientific publications have already proposed software [4] or Excel spreadsheets [5][6][7] as tools to simulate chromatographic separations. Some are commercial, for example, Drylab (Molnar-Institut, Germany) and ChromSword (Iris Tech) [8], which may not be compatible with the budget of a state university.…”

Section: Introductionmentioning

confidence: 99%