Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture

Arneborg, Nils; Siegumfeldt, Henrik; Andersen, Grith H.; Nissen, Peter H.; Daria, Vincent Ricardo; Rodrigo, Peter John; Glückstad, Jesper

doi:10.1016/j.femsle.2005.03.008

Cited by 77 publications

(41 citation statements)

References 14 publications

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“…Later, mentioned that this growth arrest could be caused by a lower ability of the non-Saccharomyces yeasts to compete for space. Arneborg et al (2005) proved that the generation time of H. uvarum cells surrounded by viable S. cerevisiae cells was 15% lower than that of non-surrounded cells, showing that the confinement imposed by S. cerevisiae cells inhibited H. uvarum growth. Granchi et al (1998) also observed that growth of K. apiculata only ceased when S. cerevisiae obtained high cell concentrations, and this phenomenon was not caused by temperature or ethanol concentration.…”

Section: Discussionmentioning

confidence: 96%

Exclusion of Saccharomyces kudriavzevii from a wine model system mediated by Saccharomyces cerevisiae

Arroyo‐López

Pérez‐Través

Querol

et al. 2011

Yeast

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This study investigated the competition and potential hybrid generation between the species Saccharomyces cerevisiae and S. kudriavzevii in a wine-model environment. Our main goal was to understand why S. kudriavzevii has not been found in wine fermentations whilst their hybrids are present. Auxotrophic mutants (Ura − and Lys − ) were used to favour the selection of hybrids and to specifically differentiate the two species in mixed fermentations carried out at different temperatures (17• C, 24• C and 31 • C). Both yeasts showed a reduction in their maximum specific growth rates in mixed fermentations, indicating a clear antagonistic effect between the two microorganisms. Temperature played an important role in this competition. In this way, S. kudriavzevii was less affected at 17• C, but S. cerevisiae was clearly the best competitor at 31• C, preventing the growth of S. kudriavzevii. Population levels of S. kudriavzevii always significantly decreased in the presence of S. cerevisiae. Ethanol was measured throughout the fermentations and in all cases S. kudriavzevii growth was arrested when ethanol levels were <5 g/l, indicating that this compound did not influence the competitive exclusion of S. kudriavzevii. Killer factors were also discarded due to the K − R − phenotype of both strains. Finally, no prototrophic interspecific hybrids were isolated in small-scale fermentations at any temperature assayed. Our results show that the lack of competitiveness exhibited by S. kudriavzevii, especially at high temperatures, explains the absence of this species in wine fermentations, suggesting that natural S. cerevisiae × S. kudriavzevii hybrids most likely originated in wild environments rather than in industrial fermentations.

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Section: Discussionmentioning

confidence: 96%

Exclusion of Saccharomyces kudriavzevii from a wine model system mediated by Saccharomyces cerevisiae

Arroyo‐López

Pérez‐Través

Querol

et al. 2011

Yeast

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“…Another investigation found that the internal pH of E.coli and Listeria bacteria declined because of direct trapping [19]. Even without direct exposure to the focused laser beam, yeast cells are found to divide less after 4 optical trapping [20].…”

Section: Introductionmentioning

confidence: 99%

Optical micromanipulation

2008

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“…Intriguing dynamics is observed, which calls for a more elaborate theoretical modeling of such a dynamic light-matter interacting system. This shows that the feedback control technique can adapt to any kind of overall object shape, for example, when trapping colonies of dividing cells [22], which tend to arrange themselves into a variety of unpredictable configurations. Experimental results show the adaptability of our side-view feedback algorithm to such constantly changing trapping environments (see Fig.…”

Section: Experimental Demonstrations and Resultsmentioning

confidence: 99%

Dynamic axial stabilization of counter-propagating beam-traps with feedback control

Tauro¹,

Bañas²,

Palima³

et al. 2010

Opt. Express

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Optical trapping in a counter-propagating (CP) beam-geometry provides unique advantages in terms of working distance, aberration requirements and intensity hotspots. However, its axial performance is governed by the wave propagation of the opposing beams, which can limit the practical geometries. Here we propose a dynamic method for controlling axial forces to overcome this constraint. The technique uses computervision object tracking of the axial position, in conjunction with softwarebased feedback, for dynamically stabilizing the axial forces. We present proof-of-concept experiments showing real-time rapid repositioning coupled with a strongly enhanced axial trapping for a plurality of particles of varying sizes. We also demonstrate the technique's adaptability for real-time reconfigurable feedback-trapping of a dynamically growing structure that mimics a continuously dividing cell colony. Advanced implementation of this feedback-driven approach can help make CP-trapping resistant to a host of perturbations such as laser fluctuations, mechanical vibrations and other distortions emphasizing its experimental versatility.

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Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture

Cited by 77 publications

References 14 publications

Exclusion of Saccharomyces kudriavzevii from a wine model system mediated by Saccharomyces cerevisiae

Exclusion of Saccharomyces kudriavzevii from a wine model system mediated by Saccharomyces cerevisiae

Optical micromanipulation

Dynamic axial stabilization of counter-propagating beam-traps with feedback control

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