Interactive network pharmacology and electrochemical analysis reveals electron transport-mediating characteristics of Chinese medicine formula Jing Guan Fang

Tsai, Po-Wei; Mailem, Ryan Christian; Tayo, Lemmuel L.; Hsueh, Chung-Chuan; Tseng, Chi-Chun; Chen, Bor-Yann

doi:10.1016/j.jtice.2023.104898

Cited by 8 publications

(1 citation statement)

References 59 publications

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“…The total phytochemical contents of PF extracts were assessed according to Tsai et al [ 95 ] with slight modifications. A 1000 μg/mL stock solution of crude PF extracts was prepared for all assays.…”

Section: Methodsmentioning

confidence: 99%

An In Vitro Evaluation and Network Pharmacology Analysis of Prospective Anti-Prostate Cancer Activity from Perilla frutescens

Garcia,

Huang,

De Castro-Cruz

et al. 2023

Plants

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Perilla frutescens (L.) Britt. is extensively cultivated in East Asia as a dietary vegetable, and nutraceuticals are reportedly rich in bioactive compounds, especially with anticancer activities. This study explored the in vitro cytotoxic effects of P. frutescens parts’ (stems, leaves, and seeds) extracts on prostate cancer cells (DU-145) and possible interactions of putative metabolites to related prostate cancer targets in silico. The ethanol extract of P. frutescens leaves was the most cytotoxic for the prostate cancer cells. From high-performance liquid chromatography analysis, rosmarinic acid was identified as the major metabolite in the leaf extracts. Network analysis revealed interactions from multiple affected targets and pathways of the metabolites. From gene ontology enrichment analysis, P. frutescens leaf metabolites could significantly affect 14 molecular functions and 12 biological processes in five cellular components. Four (4) KEGG pathways, including for prostate cancer, and six (6) Reactome pathways were shown to be significantly affected. The molecular simulation confirmed the interactions of relevant protein targets with key metabolites, including rosmarinic acid. This study could potentially lead to further exploration of P. frutescens leaves or their metabolites for prostate cancer treatment and prevention.

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Section: Methodsmentioning

confidence: 99%