Interactive effects of temperature and copper toxicity on photosynthetic efficiency and metabolic plasticity in Scenedesmus quadricauda (Chlorophyceae)

Yong, Wai-Kuan; Sim, Kae-Shin; Poong, Sze-Wan; Dong, Wei; Phang, Siew‐Moi; Lim, Phaik-Eem

doi:10.1007/s10811-018-1574-3

Cited by 20 publications

(12 citation statements)

References 62 publications

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“…Similar to our results, Yong et al. () also observed a reduction in Ek values and increase in NPQ in Scenedesmus quadricauda exposed to copper (total metal concentration reported), indicating the activation of the xanthophyll cycle, which gets into action under oxidative stress. In the present study this was confirmed by the gradual NPQ increase and carotenoid content in the higher end of Cu 2+ concentrations.…”

Section: Discussionsupporting

confidence: 92%

The effects of copper on photosynthesis and biomolecules yield in Chlorolobion braunii

Baracho

Silva

Lombardi

2019

Journal of Phycology

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Our knowledge of the effects of copper on microalgal physiology is largely based on studies conducted with high copper concentrations; much less is known when environmentally relevant copper levels come into question. Here, we evaluated the physiology of Chlorolobion braunii exposed to free copper ion concentrations between 5.7 × 10−9 and 5.0 × 10−6 mol · L−1, thus including environmentally relevant values. Population growth and maximum photosynthetic quantum yield of PSII were determined daily during the 96 h laboratory controlled experiment. Exponentially‐growing cells (48 h) were analyzed for effective quantum yield and rapid light curves (RLC), and total lipids, proteins, carbohydrates, chlorophyll a and carotenoids were determined. The results showed that growth rates and population density decreased gradually as copper increased in experiment, but the photosynthetic parameters (maximum and effective quantum yields) and photochemical quenching (qP) decreased only at the highest free copper concentration tested (5.0 × 10−6 mol · L−1); nonphotochemical quenching (NPQ) increased gradually with copper increase. The RLC parameters Ek and rETRmax were inversely proportional to copper concentration, while α and Im decreased only at 5.0 × 10−6 mol · L−1. The effects of copper in biomolecules yield (mg · L−1) varied depending on the biomolecule. Lipid yield increased at free copper concentration as low as 2.5 × 10−8 mol · L−1, but proteins and carbohydrates were constant throughout.

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Section: Discussionsupporting

confidence: 92%

The effects of copper on photosynthesis and biomolecules yield in Chlorolobion braunii

Baracho

Silva

Lombardi

2019

Journal of Phycology

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“…The nonphotochemical quenching parameters can be applied to estimate a share of energy dissipation within the antenna system, which is a self-protection mechanism of cells under excess light energy (Roach and Krieger-Liszkay, 2014;Xu et al, 2019). The excess light energy is eliminated by non-photochemical quenching to protect the normal progress of the photochemical reaction (Yong et al, 2018). Therefore, non-photochemical quenching parameters always increase to protect photosynthesis under mild stress or in the early stage of severe stress by quenching (e.g., pH-gradient build-up, LHC 2 phosphorylation and zeaxanthin formation) and recovery (such as a stressadapting D1 protein) mechanisms, but decrease with further damage (Cummings et al, 2018;Nowicka, 2019).…”

Section: Discussionmentioning

confidence: 99%

Combined Effects of Allelopathic Polyphenols on Microcystis aeruginosa and Response of Different Chlorophyll Fluorescence Parameters

Huang

Zhu

et al. 2020

Front. Microbiol.

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Polyphenols are allelochemicals secreted by aquatic plants that effectively control cyanobacteria blooms. In this study, sensitive response parameters (including CFPs) of Microcystis aeruginosa were explored under the stress of different polyphenols individually and their combination. The combined effects on M. aeruginosa were investigated based on the most sensitive parameter and cell densities. For pyrogallic acid (PA) and gallic acid (GA), the sensitivity order of parameters based on the EC50 values (from 0.73 to 3.40 mg L–1 for PA and from 1.05 to 2.68 mg L–1 for GA) and the results of the hierarchical cluster analysis showed that non-photochemical quenching parameters [NPQ, qN, qN(rel) and qCN] > photochemical quenching parameters [YII, qP, qP(rel) and qL] or others [Fv/Fm, F’v/F’m, qTQ and UQF(rel)] > cell densities. CFPs were not sensitive to ellagic acid (EA) and (+)-catechin (CA). The sensitivity order of parameters for M. aeruginosa with PA-GA mixture was similar to that under PA and GA stress. The quantitative (Toxicity Index, TI) and qualitative (Isobologram representation) methods were employed to evaluate the combined effects of PA, GA, and CA on M. aeruginosa based on cell densities and NPQ. TI values based on the EC50 cells suggested the additive effects of binary and multiple polyphenols, but synergistic and additive effects according to the EC50 NPQ (varied from 0.16 to 1.94). In terms of NPQ of M. aeruginosa, the binary polyphenols exhibited synergistic effects when the proportion of high toxic polyphenols PA or GA was lower than 40%, and the three polyphenols showed a synergistic effect only at the ratio of 1:1:1. Similar results were also found by isobologram representation. The results showed that increasing the ratio of high toxic polyphenols would not enhance the allelopathic effects, and the property, proportion and concentrations of polyphenols played an important role in the combined effects. Compared with cell densities, NPQ was a more suitable parameter as evaluating indicators in the combined effects of polyphenols on M. aeruginosa. These results could provide a method to screen the allelochemicals of polyphenols inhibiting cyanobacteria and improve the inhibitory effects by different polyphenols combined modes.

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“…11 An increase in amino acid pools under stress conditions has been also reported in sulfur-depleted C. reinhardtii cells 56 and in C. reinhardtii under hyperosmotic stress. 57 However, the present findings for IHg and MeHg are opposite to the decrease of some of the amino acids (lysine, arginine, and glutamine) observed in copper exposure of other green algae Chlorella sp., 58,59 Scenedesmus quadricauda, 60 and diatom Tabellaria flocculosa (Roth) Kuẗzing. 61 Nucleobase/-Tide/-Side Metabolism.…”

Section: ■ Introductionmentioning

confidence: 99%

Metabolomic Responses of Green Alga Chlamydomonas reinhardtii Exposed to Sublethal Concentrations of Inorganic and Methylmercury

Slaveykova

Majumdar

Regier

et al. 2021

Environ. Sci. Technol.

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Metabolomics characterizes low-molecular-weight molecules involved in different biochemical reactions and provides an integrated assessment of the physiological state of an organism. By using liquid chromatography−mass spectrometry targeted metabolomics, we examined the response of green alga Chlamydomonas reinhardtii to sublethal concentrations of inorganic mercury (IHg) and monomethylmercury (MeHg). We quantified the changes in the levels of 93 metabolites preselected based on the disturbed metabolic pathways obtained in a previous transcriptomics study. Metabolites are downstream products of the gene transcription; hence, metabolite quantification provided information about the biochemical status of the algal cells exposed to Hg compounds. The results showed that the alga adjusts its metabolism during 2 h exposure to 5 × 10 −9 and 5 × 10 −8 mol L −1 IHg and MeHg by increasing the level of various metabolites involved in amino acid and nucleotide metabolism, photorespiration, and tricarboxylic acid (TCA) cycle, as well as the metabolism of fatty acids, carbohydrates, and antioxidants. Most of the metabolic perturbations in the alga were common for IHg and MeHg treatments. However, the exposure to IHg resulted in more pronounced perturbations in the fatty acid and TCA metabolism as compared with the exposure to MeHg. The observed metabolic perturbations were generally consistent with our previously published transcriptomics results for C. reinhardtii exposed to the comparable level of IHg and MeHg. The results highlight the potential of metabolomics for toxicity evaluation, especially to detect effects at an early stage of exposure prior to their physiological appearance.

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Interactive effects of temperature and copper toxicity on photosynthetic efficiency and metabolic plasticity in Scenedesmus quadricauda (Chlorophyceae)

Cited by 20 publications

References 62 publications

The effects of copper on photosynthesis and biomolecules yield in Chlorolobion braunii

The effects of copper on photosynthesis and biomolecules yield in Chlorolobion braunii

Combined Effects of Allelopathic Polyphenols on Microcystis aeruginosa and Response of Different Chlorophyll Fluorescence Parameters

Metabolomic Responses of Green Alga Chlamydomonas reinhardtii Exposed to Sublethal Concentrations of Inorganic and Methylmercury

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