Interactive effects of CDKN2B‐AS1 gene polymorphism and habitual risk factors on oral cancer

Abstract

Oral squamous cell carcinoma (OSCC) is a common malignant disease associated with a high mortality rate and heterogeneous disease aetiology. Cyclin dependent kinase inhibitor 2B antisense RNA 1 (CDKN2B‐AS1), is a long noncoding RNA that has been shown to act as a scaffold, sponge, or signal hub to promote carcinogenesis. Here, we attempted to assess the effect of CDKN2B‐AS1 single‐nucleotide polymorphisms (SNPs) on the susceptibility to OSCC. Five CDKN2B‐AS1 SNPs, including rs564398, rs1333048, rs1537373, rs2151280 and rs8181047, were analysed in 1060 OSCC cases and 1183 cancer‐free controls. No significant association of these five SNPs with the risk of developing OSCC was detected between the case and control group. However, while examining the clinical characteristics, patients bearing at least one minor allele of rs1333048 (CA and CC) were more inclined to develop late‐stage (stage III/IV, adjusted OR, 1.480; 95% CI, 1.129–1.940; p = 0.005) and large‐size (greater than 2 cm in the greatest dimension, adjusted OR, 1.347; 95% CI, 1.028–1.765; p = 0.031) tumours, as compared with those homologous for the major allele (AA). Further stratification analyses demonstrated that this genetic correlation with the advanced stage of disease was observed only in habitual betel quid chewers (adjusted OR, 1.480; 95% CI, 1.076–2.035; p = 0.016) or cigarette smokers (adjusted OR, 1.531; 95% CI, 1.136–2.063; p = 0.005) but not in patients who were not exposed to these major habitual risks. These data reveal an interactive effect of CDKN2B‐AS1 rs1333048 with habitual exposure to behavioural risks on the progression of oral cancer.

Impact of CD44 genetic variants on clinicopathological characteristics of uterine cervical cancer patients

“…The allelic determination of 6 CD44 genetic variants, rs1425802 (assay ID: C_7618925_20), rs187115 (assay ID: C_779820_10), rs713330 (assay ID: C_779798_20), rs11821102 (assay ID: C_2143187_10), rs10836347 (assay ID: C_32034604_10), and rs13347 (assay ID: C_7619022_10) were detected by ABI StepOne Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) as well as defined by SDS version 3.0 software (Applied Biosystems) with the TaqMan assay 25 .…”

Impact of LINC00312 gene polymorphism coupled with habitual risks on buccal mucosa cancer

“…The latest breakthroughs in the understanding of long non-coding RNA (lncRNA) functions have revolutionized the scope of molecular genetics 11 . Until now, a considerable number of lncRNAs are found to be linked to a myriad of disorders 12 , 13 , including oral cancer 13 - 16 . Among these cancer-associated lncRNAs, LINC00312, long intergenic non-coding RNA 312, was reported to be significantly downregulated in nasopharyngeal carcinoma (NPC), and its levels were negatively correlated with tumor size but positively correlated with lymph node metastasis in NPC 17 .…”

Evaluation of the clinical significance of long non-coding RNA MALAT1 genetic variants in human lung adenocarcinoma

“…Non-coding RNAs (ncRNAs) are further divided into two categories according to the length, less than 200 bp (Small non-coding RNAs; sncRNAs) and greater than 200 bp (Long non-coding RNAs; lncRNAs), respectively [12]. Accumulating evidence has shown that long noncoding RNAs, which have been identified as oncogenes or tumor suppressors, play crucial roles in biological processes and are involved in tumorigenesis processes, including uncontrolled proliferation, escape cell death and tumor metastasis [13][14][15][16]. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) lncRNA, which was initially identified as an oncogene in a study of NSCLC, is situated at 11q13 [17].…”