Interactive effects of ozone exposure and nitrogen addition on the rhizosphere bacterial community of poplar saplings

Wang, Qi; Li, Zhengzhen; Li, Xuewei; Qin, Peiyong; Yuan, Xiangyang; Agathokleous, Evgenios; Feng, Zhaozhong

doi:10.1016/j.scitotenv.2020.142134

Cited by 32 publications

(16 citation statements)

References 89 publications

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“…Previous studies showed that infection by Melampsora species could induce flavonoid pathway-related genes (83-84) whereas identification of these plant metabolites in shaping the phyllosphere microbiome and immune-related regulatory mechanism remains great challenges. By comparing the effects of elevated O 3 and N addition on rhizosphere soil microbiome community of poplar clone '107', a study has shown that N addition may have a more direct effect on the belowground system than elevated O 3 (77). Our finding suggests that elevated O 3 may have a more direct effect on the aboveground system, as elevated O 3 exert a significant impact on the composition of phyllosphere fungal community than N addition.…”

Section: 3mentioning

confidence: 64%

“…The host plant could directly affect the plant-associate microbial community by modifying the chemical features in surrounding environment (76). For instance, a recent study showed that N addition significantly decreased the rhizosphere soil bacterial α-diversity of the poplar clone '107' and this negative effect could be alleviated by elevated O 3 (77). Compared with rhizosphere, it is rarely known about how the role of abiotic factors in aerial tree surface, which is characterized as extremely poor in nitrogen and carbon sources and prone to rapid fluctuation (66,78).…”

Section: 3mentioning

confidence: 99%

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Elevated ozone concentration and nitrogen addition increase poplar rust severity by shifting the phyllosphere microbial community

Tao

Zhang

Tian

et al. 2021

Preprint

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The tropospheric ozone and nitrogen deposition are two major environmental pollutants. Numerous studies have focused on the negative impacts of elevated O3 and the complementary effect of soil N addition to tree physiological characteristics. However, it was notoriously ignored of how elevated O3 with N addition affect tree immunity in face of pathogen infection, as well as of the important roles of phyllosphere microbiome community in host-pathogen-environment interplay. Here, we examined the effects of elevated O3 and soil N addition on poplar leaf rust (Melampsora larici-populina) severity of two susceptible hybrid poplars (clone ‘107’: Populus euramericana cv. ‘74/76’; clone ‘546’: P. deltoides × P. cathayana) in Free-Air-Controlled-Environment plots, besides, the link between Mlp-susceptibility and changes in microbial community was determined using Miseq amplicon sequencing. Rust severity of clone ‘107’ significantly increased under elevated O3 or N addition only, however, the negative impact of elevated O3 could be significantly alleviated when simultaneously conducting N addition, likewise, this trade-off was also found in its phyllosphere microbial α-diversity responding to elevated O3 and N addition. However, the rust severity of clone ‘546’ did not significantly differ in the cases of elevated O3 and N addition. Mlp-infection altered microbial community composition and increased its sensitivity to elevated O3 assessed by significantly different abundance of taxa. Elevated O3 and N addition reduced the complexity of microbial community, which may explain the increased severity of poplar rust. These findings demonstrated that poplars need shifting phyllosphere microbial associations to optimize plant immunity in response to environmental changes.

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Section: 3mentioning

confidence: 64%

Section: 3mentioning

confidence: 99%

Elevated ozone concentration and nitrogen addition increase poplar rust severity by shifting the phyllosphere microbial community

Tao

Zhang

Tian

et al. 2021

Preprint

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“…A bacteria-based primer set 515F/806R [24] was used to amplify about 300-bp fragments of the V4 hypervariable region of the bacterial 16S rRNA genes. PCR was performed according to the method described by Wang et al [25]. Then, the high-throughput paired-end sequencing was performed with the Illumina MiSeq PE250 platform (Illumina, San Diego, CA).…”

Section: Dna Extraction and Miseq Sequencingmentioning

confidence: 99%

“…The raw sequences of 16S rRNA gene MiSeq sequencing were processed on the i-Sanger platform (http://www.i-sanger.com/) as described by Wang et al [25]. In order to avoid heterogeneity related to different sequencing depths, a randomly-selected subset of 11,000 sequences per sample was performed for down-stream analysis.…”

Section: Statistical Analysesmentioning

confidence: 99%

Bacterial communities in habitats of Dongchuan, China: Their role in slate weathering in triggering and flowing areas of debris flow

Wang¹,

Cheng²,

Agathokleous³

et al. 2021

Preprint

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Debris flow is one of the most severe disasters in China’s mountainous areas. Landslide soil materials are deposited on steep hill terrains or in channels and, once triggered by heavy rainfall, they can evolve into lethal debris flows. Some studies have suggested a prominent role of rock weathering in the formation of large landslides. In the present study, the a-diversity, composition, and weathering potential of bacterial communities inhabiting surfaces of slate rocks from the triggering area (altered less or more) and slate rocks from the flowing area of debris flows were studied. We found that a-diversity indices were positively correlated with the organic matter content, and Firmicutes accounted for about 66% of the total bacterial community. The variation in bacterial community composition was majorly driven by available Ca. The highest relative abundances of genetic families associated with organic acid production, flagellar assembly and carbonic anhydrase were found in surfaces of less-altered slate rocks from the triggering area by using PICRUSt. All the bacterial strains isolated from rock surface have the ability to accelerate the weathering of slate, among which Pantoea and Pseudomonas genera showing the highest ability to enhance the dissolutions of Fe, Si, Al, and Ca from slate. These results indicated an important role of the rock surface-inhabiting bacterial communities in the weathering of slate in both triggering and flowing areas of debris flow. This study also provides new insights into the biological characterizations of rocks in debris flow areas of Dongchuan, China.

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“…The few reports to date exploring O 3 ‐related changes in the composition of soil microbial communities associated with agricultural systems have yielded inconsistent results, with positive and negative effects on wheat and rice rhizosphere microbial communities having been reported (Bao et al, 2015; Changey et al, 2018; Chen et al, 2010; Feng et al, 2015; Li et al, 2012, 2013). In some cases, O 3 was found to suppress microbial diversity (Chen et al, 2019; Wang et al, 2021), whereas in other cases, it altered such diversity (Ueda et al, 2016) or had no effect (Li et al, 2013). The variable findings may be related to the species‐specific effects of O 3 or to differences in the dose and duration of O 3 exposure (Agathokleous et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Soil pH drives poplar rhizosphere soil microbial community responses to ozone pollution and nitrogen addition

Yin

Zhou

et al. 2021

European J Soil Science

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Ground‐level ozone (O3) pollution frequently coincides with the deposition of anthropogenic nitrogen (N), and both factors can influence the structure and functionality of both above‐ and belowground ecosystems. Elevated O3 levels have been shown to adversely impact plants in many prior reports, but the interacting effects of high O3 levels and N addition on exposed plants remain to be clearly defined, and the changes in rhizosphere microbial community composition in this context have yet to be studied. The direct and indirect mechanisms and interactions among plants, microbes, and the soil that shape these O3 and N responses are also poorly understood. Herein, we explored the interactive effects of O3 exposure (five levels) and soil N (four levels) on the composition of rhizosphere soil microbial communities associated with poplar trees (Populus euramericana cv. ‘74/76’). In these analyses, exposure to higher levels of O3 was linked to significant decreases in bacteria, fungi, arbuscular mycorrhizal fungi, and to a reduction in the ratio of fungi‐to‐bacteria, whereas soil N addition had no impact on these parameters. No interactive effects between O3 and N were observed in the context of alterations in soil microbial community composition, and equivalent performance was observed for concentration‐based (AOT40, cumulative exposure to hourly O3 concentrations >40 ppb) and flux‐based [POD1 and POD7, cumulative stomatal uptake of O3 > 1 or 7 nmol O3 m−1 PLA (projected leaf area) s−1] dose–response analyses. Structural equation modelling revealed that changes in the composition of the microbial community were attributable to changes in soil pH but unrelated to plant characteristics. Overall, these findings indicated that increased O3 levels can induce soil alkalinisation and thereby influence soil microbial communities such that soil pH is a reliable predictor of O3 pollution‐related changes in these communities. Highlights Elevated O3 exhibited an overall negative influence on microorganisms. AOT40‐, POD1‐ and POD7‐based dose–responses performed equally well. Soil pH is a reliable predictor of O3 pollution‐related changes in microbial communities. N addition failed to affect O3 effects on soil microbial community.

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Interactive effects of ozone exposure and nitrogen addition on the rhizosphere bacterial community of poplar saplings

Cited by 32 publications

References 89 publications

Elevated ozone concentration and nitrogen addition increase poplar rust severity by shifting the phyllosphere microbial community

Elevated ozone concentration and nitrogen addition increase poplar rust severity by shifting the phyllosphere microbial community

Bacterial communities in habitats of Dongchuan, China: Their role in slate weathering in triggering and flowing areas of debris flow

Soil pH drives poplar rhizosphere soil microbial community responses to ozone pollution and nitrogen addition

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