Interactive effects of dietary composition and hormonal treatment on reproductive development of cultured female European eel, Anguilla anguilla

Silva, Filipa F.G. da; Støttrup, Josianne; Tveiten, Helge; Tomkiewicz, Jonna

doi:10.1016/j.anireprosci.2016.05.007

Cited by 18 publications

(13 citation statements)

References 42 publications

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“…The earliest life stages of European eel have not been encountered in nature, thus research needs to focus on laboratory studies to overcome current bottlenecks and gain knowledge about the species physiology and ecology. With recent advances in assisted reproduction (Butts et al, 2014;Müller et al, 2016;da Silva et al, 2016) and larval culture of European eel (Politis et al, 2014a;Butts et al, 2016) this is now possible and warrants further investigation. In addition, the European eel genome has recently been sequenced (Henkel et al, 2012), which offers new and emerging perspectives for fundamental molecular studies in eel biology (Rozenfeld et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae

Politis

Servili

Mazurais

et al. 2018

General and Comparative Endocrinology

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Thyroid hormones (THs) are key regulators of growth, development, and metabolism in vertebrates and influence early life development of fish. TH is produced in the thyroid gland (or thyroid follicles) mainly as T4 (thyroxine), which is metabolized to T3 (3,5,3'-triiodothyronine) and T2 (3,5-diiodothyronine) by deiodinase (DIO) enzymes in peripheral tissues. The action of these hormones is mostly exerted by binding to a specific nuclear thyroid hormone receptor (THR). In this study, we i) cloned and characterized thr sequences, ii) investigated the expression pattern of the different subtypes of thrs and dios, and iii) studied how temperature affects the expression of those genes in artificially produced early life history stages of European eel (Anguilla anguilla), reared in different thermal regimes (16, 18, 20 and 22 °C) from hatch until first-feeding. We identified 2 subtypes of thr (thrα and thrβ) with 2 isoforms each (thrαA, thrαB, thrβA, thrβB) and 3 subtypes of deiodinases (dio1, dio2, dio3). All thr genes identified showed high similarity to the closely related Japanese eel (Anguilla japonica). We found that all genes investigated in this study were affected by larval age (in real time or at specific developmental stages), temperature, and/or their interaction. More specifically, the warmer the temperature the earlier the expression response of a specific target gene. In real time, the expression profiles appeared very similar and only shifted with temperature. In developmental time, gene expression of all genes differed across selected developmental stages, such as at hatch, during teeth formation or at first-feeding. Thus, we demonstrate that thrs and dios show sensitivity to temperature and are involved in and during early life development of European eel.

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Section: Introductionmentioning

confidence: 99%

Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae

Politis

Servili

Mazurais

et al. 2018

General and Comparative Endocrinology

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“…This indicated that DHA may be superior to EPA in promoting fish steroid hormone synthesis. Very little information has been available about the relative effectiveness of DHA and EPA in regulating fish steroidogenesis, although several in vivo studies with fish species such as gilthead seabream (Sparus aurata), silver pomfret (Pampus argenteus) and European eel have investigated the effects of total LC-PUFA or n-3 LC-PUFA on sex steroid production (24)(25)(26) . A feeding trial with Siberian sturgeon (Acipenser baeri) showed that serum oestradiol was higher in females fed the diet with a DHA:EPA ratio of 1•9:1 compared with the ratio of 1:1•9 (22) , providing another evidence for the superiority of DHA to EPA in inducing sex steroid production.…”

Section: Discussionmentioning

confidence: 99%

“…In both terrestrial animals and fish species, less information has been available about the modulation of steroidogenesis by DHA and EPA (19)(20)(21)(22) . Moreover, contradictory results have been reported in fish studies (23)(24)(25)(26) . The present study will provide new insight into the regulatory effects of DHA and EPA on animal gonadal steroidogenesis.…”

mentioning

confidence: 98%

Effects of different dietary DHA:EPA ratios on gonadal steroidogenesis in the marine teleost, tongue sole (Cynoglossus semilaevis)

Cao

Wei

et al. 2017

Br J Nutr

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The present study was conducted to investigate the effects of dietary DHA and EPA on gonadal steroidogenesis in mature females and males, with a feeding trial on tongue sole, a typical marine teleost with sexual dimorphism. Three experimental diets differing basically in DHA:EPA ratio, that is, 0·68 (diet D:E-0·68), 1·09 (D:E-1·09) and 2·05 (D:E-2·05), were randomly assigned to nine tanks of 3-year-old tongue sole (ten females and fifteen males in each tank). The feeding trail lasted for 90 d before and during the spawning season. Fish were reared in a flowing seawater system and fed to apparent satiation twice daily. Compared with diet D:E-0·68, diet D:E-1·09 significantly enhanced the oestradiol production in females, whereas diet D:E-2·05 significantly enhanced the testosterone production in males. In ovaries, diet D:E-1·09 induced highest mRNA expression of follicle-stimulating hormone receptor (FSHR), steroidogenic acute regulatory protein, 17α-hydroxylase (P450c17) and 3β-hydroxysteroid dehydrogenase (3β-HSD). In testes, diet 2·05 resulted in highest mRNA expression of FSHR, cholesterol side-chain cleavage enzyme, P450c17 and 3β-HSD. Fatty acid profiles in fish tissues reflected closely those of diets. Female fish had more gonadal EPA content but less DHA content than male fish, whereas there was a reverse observation in liver. In conclusion, the dietary DHA:EPA ratio, possibly combined with the dietary EPA:arachidonic acid ratio, differentially regulated sex steroid hormone synthesis in mature female and male tongue soles. Females seemed to require more EPA but less DHA for the gonadal steroidogenesis than males. The results are beneficial to sex-specific nutritive strategies in domestic teleost.

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“…in CPE injected eels kept at 18 °C, 25% of fish only contained lipid droplets in their oocytes after 4 weeks of CPE injection (Mazzeo et al 2014), while in another study oocytes of 20% of CPE injected eels did not contain yolk globules after 4 weeks at 20 °C (Pérez et al 2011). Even after 13 weeks of SPE treatment ~12% of the eel of yet another experiment did not present yolk globules (da Silva et al 2016), although these should be considered as non-responders. Furthermore, at 15 ºC oocytes without yolk globules appears to be the norm after 3 weeks of CPE injection (Nowosad et al 2015).…”

Section: Natural Eel Sexual Maturation Factorsmentioning

confidence: 87%

“…Nutritional status has been observed to influence fertility in animals for more than a century (Darwin 1859) and it is likely a natural cue on which eels bases their individual decision of initiating migration (Durif et al 2005). Therefore, this factor has received some attention in the field of eel reproduction (Støttrup et al 2013;Baeza et al 2014Baeza et al , 2015ada Silva et al 2016;Støttrup et al 2016). In particular, da Silva et al (2016) showed that dietary fatty acids can influence gonadal development and individual eels response to hormonal treatment (Baeza et al 2015a;da Silva et al 2016).…”

Section: Natural Eel Sexual Maturation Factorsmentioning

confidence: 99%

Development of innovative methods for induction of European eel (Anguilla anguilla) spermatogenesis

Rozenfeld¹

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Interactive effects of dietary composition and hormonal treatment on reproductive development of cultured female European eel, Anguilla anguilla

Cited by 18 publications

References 42 publications

Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae

Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae

Effects of different dietary DHA:EPA ratios on gonadal steroidogenesis in the marine teleost, tongue sole (Cynoglossus semilaevis)

Development of innovative methods for induction of European eel (Anguilla anguilla) spermatogenesis

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